Cervid Prion Protein Polymorphisms: Role in Chronic Wasting Disease Pathogenesis

Chronic wasting disease (CWD) is a prion disease found in both free-ranging and farmed cervids. Susceptibility of these animals to CWD is governed by various exogenous and endogenous factors. Past studies have demonstrated that polymorphisms within the prion protein (PrP) sequence itself affect an animal’s susceptibility to CWD. PrP polymorphisms can modulate CWD pathogenesis in two ways: the ability of the endogenous prion protein (PrP^C) to convert into infectious prions (PrP^Sc) or it can give rise to novel prion strains. In vivo studies in susceptible cervids, complemented by studies in transgenic mice expressing the corresponding cervid PrP sequence, show that each polymorphism has distinct effects on both PrP^C and PrP^Sc. It is not entirely clear how these polymorphisms are responsible for these effects, but in vitro studies suggest they play a role in modifying PrP epitopes crucial for PrP^C to PrP^Sc conversion and determining PrP^C stability. PrP polymorphisms are unique to one or two cervid species and most confer a certain degree of reduced susceptibility to CWD. However, to date, there are no reports of polymorphic cervid PrP alleles providing absolute resistance to CWD. Studies on polymorphisms have focused on those found in CWD-endemic areas, with the hope that understanding the role of an animal’s genetics in CWD can help to predict, contain, or prevent transmission of CWD.     

Auto-generation of IEC standard PLC code using t-MPSG

The objective of this paper is to reduce the development time of a PLC (Programmable Logic Controller) by automating the task of code generation. For this purpose, we applied t-MPSG (Timed-Message Based Part State Graph). The t-MPSG is an extended finite state automata used to model and generate an execution module for a real-time shop floor controller system. In our proposed method, t-MPSG is used to model the formal specification of the controller system that can be translated into textual structure. After the verification of the t-MPSG model, it can be used as an input to the plc-builder tool. The plc-builder tool is an extended version of a conventional MPSG simulator. It can be used to translate the textual structure of the t-MPSG into an IEC standard PLC code. Finally, the generated code can be downloaded to a PLC emulator or a PLC device for the purpose of simulation and execution. The similarity in the hierarchical structure of the t-MPSG and the IEC standard PLC program has made it convenient to transform from one form to another. Furthermore, an illustration of the methodology to auto-generate IEC standard PLC code using t-MPSG is explained with a suitable example. Recommended by Editorial Board member Young Soo Suh under the direction of Editor Jae Weon Choi. This work was partially supported by Defense Acquisition Program Administration and Agency for Defense Development under the contract (UD080042AD). Devinder Thapa is a Postdoc Research Fellow in the Department of Industrial & information systems at Ajou University, Korea. He completed his Ph.D. from Ajou University in Industrial and Information Systems Engineering. His area of research is related to manufacturing automation and intelligent decision support systems. Chang Mok Park is a Professor in the Department of Technology & Systems Management at Induk Institute of Technology. He completed his Ph.D. in 2002 from Ajou University in Industrial Engineering. His research interest is related to manufacturing optimization, discrete event system simulation and signal analysis. Sang C. Park is an Associate Professor in the Department of Industrial & Information Systems Engineering at Ajou University. He received his B.S., M.S., and Ph.D. degrees from KAIST in 1994, 1996, and 2000, respectively, all in Industrial Engineering. His research interests include geometric algorithms in CAD/CAM, process planning, engineering knowledge management, and discrete event system simulation. Gi-Nam Wang is the Head and a Professor in the Department of Industrial & Information Systems Engineering at Ajou University, Korea. He completed his Ph.D. in 1992 from Texas A&M University, in Industrial Engineering. He has worked as Visiting Professor at University of Texas at Austin during 2000–2001. His area of research is related to Intelligent Information & manufacturing systems, system integration & automation, e-Business solutions and image processing.     

Foley catheters functionalised with a synergistic combination of antibiotics and silver nanoparticles resist biofilm formation

Foley catheters are inevitable in health care unit. Pathogens colonise and form biofilm on catheter causing catheter‐associated urinary tract infection. Therefore, the authors aimed to functionalise catheter to resist biofilm formation. The authors impregnated urinary catheters with a synergistic combination of antibiotics and silver nanoparticles (SNPs) to evaluate antibiofilm efficacy in vitro and in vivo. SNPs were synthesised using Spirulina platensis. Synergy between the SNPs and antibiotics was determined by the checker‐board method. In vivo efficacy of the functionalised catheters was assessed in mice. Liver and kidney function tests of mice were performed. The in vitro anti‐adherence activity of the functionalised catheters was evaluated after 2 years. Nanoparticle sizes were 42–75 nm. Synergistic activity was observed among SNPs (2 µg/ml), amikacin (6.25 µg/ml), and nitrofurantoin (31.25 µg/ml). In mice, catheters functionalised with combinations of antibiotics and SNPs exhibited no colonisation until Day 14. Blood, liver, and kidney tests were normal. After 2 years, catheters functionalised with antibiotics exhibited 25% inhibition of bacterial adhesion, and catheters functionalised with the nanoparticle‐antibiotic combination exhibited 90% inhibition. Impregnation of urinary catheters with a synergistic combination of antibiotics and SNPs is an efficient and promising method for preventing biofilm formation.Inspec keywords: catheters, drugs, silver, nanoparticles, nanomedicine, liver, kidney, blood, microorganisms, adhesion, biomechanics, cellular biophysicsOther keywords: Foley catheters, synergistic nanoparticle‐antibiotics combination, silver nanoparticles, biofilm formation resitance, health care unit, pathogens, urinary tract infection, SNP, Spirulina platensis, checker‐board method, liver function, kidney function, vitro antiadherence activity, amikacin, nitrofurantoin, blood, bacterial adhesion, size 42 nm to 75 nm, Ag     

Urban growth modeling of Kathmandu metropolitan region, Nepal

The complexity of urban system requires integrated tools and techniques to understand the spatial process of urban development and project the future scenarios. This research aims to simulate urban growth patterns in Kathmandu metropolitan region in Nepal. The region, surrounded by complex mountainous terrain, has very limited land resources for new developments. As similar to many cities of the developing world, it has been facing rapid population growth and daunting environmental problems. Three time series land use maps in a fine-scale (30 m resolution), derived from satellite remote sensing, for the last three decades of the 20th century were used to clarify the spatial process of urbanization. Based on the historical experiences of the land use transitions, we adopted weight of evidence method integrated in cellular automata framework for predicting the future spatial patterns of urban growth. We extrapolated urban development patterns to 2010 and 2020 under the current scenario across the metropolitan region. Depending on local characteristics and land cover transition rates, this model produced noticeable spatial pattern of changes in the region. Based on the extrapolated spatial patterns, the urban development in the Kathmandu valley will continue through both in-filling in existing urban areas and outward rapid expansion toward the east and south directions. Overall development will be greatly affected by the existing urban space, transportation network, and topographic complexity.     

Cross-layer based congestion detection and routing protocol using fuzzy logic for MANET

Mobile Ad hoc Networks (MANET) is made up of mobile devices which form an infrastructure-less network. In MANET, the devices can change its location and configure itself at any time. Hence, there is a possibility for the network to be prone to abnormal network events due to certain factors like error in the links, overflow in the buffers, layers, and so forth. So, in this paper, a cross-layer based congestion detection and routing protocol is proposed using Fuzzy logic. In this protocol, whenever a network event occurs, the kind of event occurring is recognized in order to handle it accordingly. Next, the alternate routes for data transmission are determined by applying the concept of fuzzy logic on some of the critical factors. Based on the fuzzy inference rules, appropriate routes are selected and data messages are transmitted successfully.     

Identification and enzymatic profiles of the predominant lactic acid bacteria isolated from soft‐variety Chhurpi,a traditional cheese typical of the Sikkim Himalayas

Abstract

This paper is the first report on the microbial population and predominant lactic acid bacteria (LAB) of two soft‐varieties (mild and strong flavoured) of chhurpi., a traditional cheese product of the Sikkim Himalayas. The enzymatic profiles and percentage hydrophobicity (as one criterion of potential adhesion capability) of the predominant LAB were also studied. The LAB, yeasts and viable mesophilic microbial numbers ranged from 7.6–7.9, 7.0–7.4 and 7.7–8.0 log₁₀ cfu/g respectively. The predominant LAB were identified as Lactobacillus plantarum, Lb. curvatus, Lb. fermentum, Lb. paracasei subsp. pseudoplcmtarum and Leuconostoc mesenteroides. They produced a wide spectrum of enzymes and also exhibited similar patterns of enzymatic activity between species. In comparison to the peptidases, they showed relatively weak esterase and lipase activities. No proteinase activity was detected. Most strains had a high degree of hydrophobicity. The results are discussed in relation to the possible role of the strains in food processing.     

Lignite aided dewatering of digested sewage sludge

Mechanical dewatering is commonly used to increase the solids content of municipal sludge prior to its disposal. However, if the rate of filtration is slow, mechanical dewatering can be expensive. In this study, the use of lignite to improve the sludge dewatering is investigated. The effectiveness of lignite conditioning of polyelectrolyte-flocculated sludge is examined using mechanical compression tests. Results show that lignite conditioning in conjunction with polyelectrolyte flocculation gives much better dewatering than the polyelectrolyte flocculation alone. Using Darcy's filtration theory, the specific cake resistance and permeability of the compressed cakes are obtained. Both of these parameters are significantly improved after lignite conditioning. Mercury porosimetry tests on compressed cakes show that the porosity of the lignite-conditioned sludge cake is much higher than that of the polyelectrolyte-flocculated sludge and it increases with increasing doses of lignite. The mercury porosimetry results show that the lignite pore volume of pores greater than 0.5 μm are reduced with increasing sludge ratio indicating that sludge is trapped within these pores, whereas smaller pores are unaffected. The yield stress curves for sludge, lignite and sludge-lignite mixtures show that the sludge filter cake is very compressible, but the lignite-conditioned cake has a range of compressibility which although more than lignite indicate that the cake is relatively incompressible at low pressures. Thus, lignite conditioning acts to maintain the permeability of the filter cake during compression dewatering by resisting cake compression. This leads to a trade-off between the rate of dewatering and the solids content of the compressed cake. With lignite conditioning, the dewatering rate can be increased by a factor of five for the same degree of water removal.     

Electrostatic assembly of nanoparticles and biomacromolecules

The controlled assembly of nanoparticles in thin film form on solid supports, both as monolayers and as superlattice structures, is a problem of considerable topical interest. Among the many interactions used to program the assembly of nanoparticles, electrostatic forces are particularly interesting for many reasons. This Account deals with assembling surface-modified nanoparticles in thin film form using electrostatic interactions at the air-water interface and in thermally evaporated lipid films. The generality of the electrostatic assembly protocol is demonstrated in the immobilization of DNA and proteins in lipid films.     

Prediction of semiconducting behavior in minority spin of Co2CrZ（Z=Ga,Ge,As）:LSDA

正Volume optimization was performed to obtain the theoretical lattice constants by using the generalized gradient approximation(GGA).The electronic and magnetic properties of Heusler alloys Co_2CrZ(Z = Ga,Ge, As) were investigated by using local spin density approximation(LSDA).Amongst the systems under investigation, Co_2CrGe and Co_2CrGa give 100%spin polarization at the Fermi level(E_f)- Co_2CrGe and Co_2CrGa are the most stable half-metallic ferromagnets(HMFs);their E_F lie exactly at the gap of 0.24 eV and 0.38 eV,respectively,in the spin-down channel.Even though Co_2CrAs gives a distinct and bigger gap as compared to Co_2CrGa and Co_2CrGe, its E_F is not located at the middle of the gap in the spin-down channel.We have also found that the total magnetic moments increase as the Z goes from Ga to As.The calculated density of states and band structures show the HMF character for Co_2CrGe and Co_2CrGa.