排序方式: 共有5条查询结果,搜索用时 156 毫秒
1
1.
Ximena Zottig Soultan Al‐Halifa Margaryta Babych No Quittot Denis Archambault Steve Bourgault 《Small (Weinheim an der Bergstrasse, Germany)》2019,15(33)
Peptides that self‐assemble into cross‐β‐sheet amyloid structures constitute promising building blocks to construct highly ordered proteinaceous materials and nanoparticles. Nevertheless, the intrinsic polymorphism of amyloids and the difficulty of controlling self‐assembly currently limit their usage. In this study, the effect of electrostatic interactions on the supramolecular organization of peptide assemblies is investigated to gain insights into the structural basis of the morphological diversities of amyloids. Different charged capping units are introduced at the N‐terminus of a potent β‐sheet‐forming sequence derived from the 20–29 segment of islet amyloid polypeptide, known to self‐assemble into polymorphic fibrils. By tuning the charge and the electrostatic strength, different mesoscopic morphologies are obtained, including nanorods, rope‐like fibrils, and twisted ribbons. Particularly, the addition of positive capping units leads to the formation of uniform rod‐like assemblies, with lengths that can be modulated by the charge number. It is proposed that electrostatic repulsions between N‐terminal positive charges hinder β‐sheet tape twisting, leading to a unique control over the size of these cytocompatible nanorods by protofilament growth frustration. This study reveals the high susceptibility of amyloid formation to subtle chemical modifications and opens to promising strategies to control the final architecture of proteinaceous assemblies from the peptide sequence. 相似文献
2.
3.
Margaryta K Shelyakina Oleksandr O Soldatkin Valentyna M Arkhypova Berna O Kasap Burcu Akata Sergei V Dzyadevych 《Nanoscale research letters》2014,9(1):124
A possibility of the creation of potentiometric biosensor by adsorption of enzyme urease on zeolite was investigated. Several variants of zeolites (nano beta, calcinated nano beta, silicalite, and nano L) were chosen for experiments. The surface of pH-sensitive field-effect transistors was modified with particles of zeolites, and then the enzyme was adsorbed. As a control, we used the method of enzyme immobilization in glutaraldehyde vapour (without zeolites). It was shown that all used zeolites can serve as adsorbents (with different effectiveness). The biosensors obtained by urease adsorption on zeolites were characterized by good analytical parameters (signal reproducibility, linear range, detection limit and the minimal drift factor of a baseline). In this work, it was shown that modification of the surface of pH-sensitive field-effect transistors with zeolites can improve some characteristics of biosensors. 相似文献
4.
5.
In this paper we present a tool that uses comparable corpora to find appropriate translation equivalents for expressions that
are considered by translators as difficult. For a phrase in the source language the tool identifies a range of possible expressions
used in similar contexts in target language corpora and presents them to the translator as a list of suggestions. In the paper
we discuss the method and present results of human evaluation of the performance of the tool, which highlight its usefulness
when dictionary solutions are lacking. 相似文献
1