全文获取类型
收费全文 | 155篇 |
免费 | 5篇 |
专业分类
电工技术 | 1篇 |
化学工业 | 28篇 |
金属工艺 | 2篇 |
机械仪表 | 3篇 |
建筑科学 | 13篇 |
能源动力 | 4篇 |
轻工业 | 6篇 |
水利工程 | 2篇 |
无线电 | 9篇 |
一般工业技术 | 39篇 |
冶金工业 | 27篇 |
自动化技术 | 26篇 |
出版年
2023年 | 2篇 |
2021年 | 3篇 |
2020年 | 1篇 |
2019年 | 2篇 |
2018年 | 3篇 |
2017年 | 5篇 |
2016年 | 1篇 |
2015年 | 7篇 |
2014年 | 4篇 |
2013年 | 7篇 |
2012年 | 6篇 |
2011年 | 8篇 |
2010年 | 8篇 |
2009年 | 2篇 |
2008年 | 10篇 |
2007年 | 12篇 |
2006年 | 4篇 |
2005年 | 7篇 |
2004年 | 3篇 |
2003年 | 7篇 |
2002年 | 3篇 |
2001年 | 6篇 |
2000年 | 6篇 |
1999年 | 3篇 |
1998年 | 5篇 |
1997年 | 5篇 |
1996年 | 9篇 |
1995年 | 7篇 |
1994年 | 2篇 |
1993年 | 1篇 |
1992年 | 4篇 |
1991年 | 1篇 |
1989年 | 2篇 |
1983年 | 1篇 |
1982年 | 1篇 |
1981年 | 1篇 |
1974年 | 1篇 |
排序方式: 共有160条查询结果,搜索用时 31 毫秒
1.
2.
Pieter Coenen Margriet Formanoy Marjolein Douwes Tim Bosch Heleen de Kraker 《Applied ergonomics》2014
Exposure to mechanical vibrations at work (e.g., due to handling powered tools) is a potential occupational risk as it may cause upper extremity complaints. However, reliable and valid assessment methods for vibration exposure at work are lacking. Measuring hand-arm vibration objectively is often difficult and expensive, while often used information provided by manufacturers lacks detail. Therefore, a subjective hand-arm vibration assessment method was tested on validity and inter-observer reliability. 相似文献
3.
Sun Ying-Chieh; Veenstra David L.; Kollman Peter A. 《Protein engineering, design & selection : PEDS》1996,9(3):273-281
Free energy calculations were carried out to determine the relativeunfolding free energy of the Ile96 wild type and Ala96 mutantbarnases. The total calculated free energies suggest that substitutionof Ile96 with Ala destabilizes barnase by 3.9 kcal/mol, whichis in good agreement with the independently determined experimentalvalues of 4.0 and 3.3 kcal/mol and a previous simulation. However,a decomposition of the free energy finds the dominant contributionsto this free energy arising from the noncovalent Interactionsbetween the perturbed group and distant residues of barnasein the sequence and water molecules and only a very small contributionfrom covalent interactions. This is in contrast to the previoussimulation, using the dual topology methodology, which produceda decomposition with an {small tilde}60% free energy contributionfrom changes in covalent interactions. The use of the singletopology employed in the present calculations and the dual topologyemployed in the previous study are analyzed in order to understandthe contrast between the present results and the results ofthe previous study. 相似文献
4.
Jurjen Jansen Sander Veenstra Renske Zuurveen Wouter Stol 《Behaviour & Information Technology》2016,35(5):368-379
Information technology is becoming increasingly important for entrepreneurs. Protecting their technical infrastructure and stored data is, therefore, also becoming more vital. Nevertheless, research into the safety and security of entrepreneurs in general, and online threats targeted at entrepreneurs in particular, is still limited. This study investigates to what extent self-employed entrepreneurs protect themselves against online threats, and what motivates them to do so. Based on secondary analyses on data collected from 1622 Dutch entrepreneurs, we observe that the majority implement technical and personal coping measures. By adopting protection motivation theory as a theoretical basis for our study, we found that coping appraisal explains the adoption of protective measures. Entrepreneurs are likely to implement protective measures when they believe a measure is effective, when they are capable of using internet technology, when their attitude towards online protection is positive and when they believe they are responsible for their own online security. Although the secondary analysis provides some limitations, this study offers new insights into the usage of protective measures and the motivation for taking them. These insights can help to improve information security campaigns. 相似文献
5.
While many of the developments made in high-throughput proteomics were originally applied to procaryotic and simple eucaryotic organisms, the analysis of biofluids became increasingly important as the prospect of using proteomics to discover novel biomarkers became realized. Biofluids have represented a unique challenge to proteomics as they are often present only in small amounts and, particularly in the cases of serum and plasma, can have protein concentration ranges that differ by ten orders of magnitude. While the discovery of authentic, clinically useful biomarkers in the proteomics era has been lacking, there have been a number of significant developments in the ability to more comprehensively characterize biofluid proteomes. The rapid pace of these developments suggest that the eventual discovery of clinically validate biomarkers using proteomic technologies still has a bright future. 相似文献
6.
7.
The influence of music on mood and performance while driving 总被引:1,自引:0,他引:1
van der Zwaag MD Dijksterhuis C de Waard D Mulder BL Westerink JH Brookhuis KA 《Ergonomics》2012,55(1):12-22
Mood can influence our everyday behaviour and people often seek to reinforce, or to alter their mood, for example by turning on music. Music listening while driving is a popular activity. However, little is known about the impact of music listening while driving on physiological state and driving performance. In the present experiment, it was investigated whether individually selected music can induce mood and maintain moods during a simulated drive. In addition, effects of positive, negative, and no music on driving behaviour and physiological measures were assessed for normal and high cognitive demanding rides. Subjective mood ratings indicated that music successfully maintained mood while driving. Narrow lane width drives increased task demand as shown in effort ratings and increased swerving. Furthermore, respiration rate was lower during music listening compared to rides without music, while no effects of music were found on heart rate. Overall, the current study demonstrates that music listening in car influences the experienced mood while driving, which in turn can impact driving behaviour. PRACTITIONERS SUMMARY: Even though it is a popular activity, little is known about the impact of music while driving on physiological state and performance. We examined whether music can induce moods during high and low simulated drives. The current study demonstrates that in car music listening influences mood which in turn can impact driving behaviour. The current study shows that listening to music can positively impact mood while driving, which can be used to affect state and safe behaviour. Additionally, driving performance in high demand situations is not negatively affected by music. 相似文献
8.
Arjan Oudshoorn Marjolein C. F. M. Peters Luuk A. M. van der Wielen Adrie J. J. Straathof 《Journal of chemical technology and biotechnology (Oxford, Oxfordshire : 1986)》2011,86(5):714-718
BACKGROUND: Fermentative production of 1‐butanol yields dilute aqueous solutions. Recovery of the butanol from these solutions is most commonly performed by energy‐intensive distillation. This work investigated the liquid‐liquid (L‐L) phase behavior of mixtures of butanol and water to explore the potential of using L‐L phase separation as a recovery possibility for 1‐butanol. The phase behavior is preferably influenced by compounds already present in the fermentation, such as carbohydrates and salts. RESULTS: The L‐L phase equilibria of butanol and water were determined in the presence of glucose, fructose, sucrose, NaCl, LiCl and CaCl2. The aqueous and organic phase split is more pronounced in the presence of salts than in the presence of carbohydrates. Demixing is achieved with about 0.3 kg salt kg?1 aqueous phase containing 40 g of butanol. CONCLUSION: Operation of L‐L based recovery using salts or carbohydrates requires extreme concentrations of those compounds. For feed material containing 40 g kg?1 butanol, the tested carbohydrates do not influence the phase equilibria sufficiently to allow butanol separation. Fermentative butanol concentrations up to 70 g kg?1 are required to create an effective L‐L phase split. The remaining residual aqueous carbohydrate solution might be used as feed for a following fermentation. Copyright © 2011 Society of Chemical Industry 相似文献
9.
Stepwise mobilization of focused proteins in capillary isoelectric focusing mass spectrometry 总被引:2,自引:0,他引:2
Zhang CX Xiang F Pasa-Tolić L Anderson GA Veenstra TD Smith RD 《Analytical chemistry》2000,72(7):1462-1468
A stepwise mobilization strategy has been developed for the elution of complex protein mixtures, separated by capillary isoelectric focusing (CIEF) for detection using on-line electrospray ionization mass spectrometry (ESI-MS). Carrier polyampholytes are used to establish a pH gradient as well as to control the electroosmotic flow arising from the use of uncoated fused-silica capillaries. Elution of focused protein zones is achieved by controlling the mobilization pressure and voltage, leaving the remaining protein zones focused inside the capillary. Protein zones are stepwise eluted from the capillary by changing the mobilization conditions. Stepwise mobilization improves separation resolution and simplifies coupling with multistage MS (i.e., MSn) analysis since it allows more effective temporal control of protein elution from the CIEF capillary. We also describe a modified configuration for coupling CIEF with ESI-MS using a coaxial sheath flow interface that facilitate the automation of on-line CIEF-ESI-MS analyses. The stepwise mobilization strategy is demonstrated for the analysis of standard protein mixtures and soluble E. coli lysate proteins using CIEF-ESI-MS. These results indicate that inlet pressure or voltage programming to control the elution of the protein zones from the capillary (i.e., gradient mobilization) may allow for the optimization of the mobilization conditions and provide higher resolution for CIEF separation of complex mixtures with on-line MS. 相似文献
10.
Shen Y Zhao R Belov ME Conrads TP Anderson GA Tang K Pasa-Tolić L Veenstra TD Lipton MS Udseth HR Smith RD 《Analytical chemistry》2001,73(8):1766-1775
In this study, high-efficiency packed capillary reversed-phase liquid chromatography (RPLC) coupled on-line with high-performance Fourier transform ion cyclotron resonance (FTICR) mass spectrometry has been investigated for the characterization of complex cellular proteolytic digests. Long capillary columns (80-cm) packed with small (3-micron) C18 bonded particles provided a total peak capacity of approximately 1000 for cellular proteolytic polypeptides when interfaced with an ESI-FTICR mass spectrometer under composition gradient conditions at a pressure of 10,000 psi. Large quantities of cellular proteolytic digests (e.g., 500 micrograms) could be loaded onto packed capillaries of 150-micron inner diameter without a significant loss of separation efficiency. Precolumns with suitable inner diameters were found useful for improving the elution reproducibility without a significant loss of separation quality. Porous particle packed capillaries were found to provide better results than those containing nonporous particles because of their higher sample capacity. Two-dimensional analyses from the combination of packed capillary RPLC with high-resolution FTICR yield a combined capacity for separations of > 1 million polypeptide components and simultaneously provided information for the identification of the separated components based upon the accurate mass tag concept previously described. 相似文献