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1.
ESR spin trapping was used to investigate the reaction of rabbit cytochrome P450 (P450) 1A2 with cumene hydroperoxide. Cumene hydroperoxide-derived peroxyl, alkoxyl, and carbon-centered radicals were formed and trapped during the reaction. The relative contributions of each radical adduct to the composite ESR spectrum were influenced by the concentration of the spin trap. Computer simulation of the experimental data obtained at various 5,5-dimethyl-1-pyrroline N-oxide (DMPO) concentrations was used to quantitate the contributions of each radical adduct to the composite ESR spectrum. The alkoxyl radical was the initial radical produced during the reaction. Experiments with 2-methyl-2-nitrosopropane identified the carbon-centered adducts as those of the methyl radical, hydroxymethyl radical, and a secondary carbon-centered radical. The reaction did not require NADPH-cytochrome P450 reductase or NADPH. It is concluded that the reaction involves the initial homolytic scission of the peroxide O-O bond to produce the cumoxyl radical. Methyl radicals were produced from the beta-scission of the cumoxyl radical. The peroxyl adduct was not observed in the absence of molecular oxygen. We conclude that the DMPO peroxyl radical adduct detected in the presence of oxygen was due to the methylperoxyl radical formed by the reaction of the methyl radical with oxygen. At a higher P450 concentration, a protein-derived radical adduct was also detected.  相似文献   
2.
By nature, cryptographers tend to be extremely cautious and conservative. From their perspective, spending time getting people to use a new cryptographic algorithm is roughly on par with getting involved in a land war in Asia. In some cases, however, the difficulty involved in developing a new algorithm is worth the effort. This is the case with identity-based encryption (IBE), a new type of public-key algorithm  相似文献   
3.
The cost of maintaining a software system over a long period of time far exceeds its initial development cost. Much of the maintenance cost is attributed to the time required by new developers to understand legacy systems. High-level structural information helps maintainers navigate through the numerous low-level components and relations present in the source code. Modularization tools can be used to produce subsystem decompositions from the source code but do not typically produce high-level architectural relations between the newly found subsystems. Controlling subsystem interactions is one important way in which the overall complexity of software maintenance can be reduced.We have developed a tool, called ARIS (Architecture Relation Inference System), that enables software engineers to define rules and relations for regulating subsystem interactions. These rules and relations are called Interconnection Styles and are definedusing a visual notation. The style definition is used by our tool to infer subsystem-level relations in designs being reverse engineered from source code.In this paper we describe our tool and its underlying techniques and algorithms. Using a case study, we describe how ARIS is used to reverse engineer high-level structural information from a real application.  相似文献   
4.
Zusammenfassung Aufnahmen von Lichtenbergschen Figuren mit kurzen Spannungsstößen zeigen, daß bisher bekanntgewordene Bilder solcher Entladungsfiguren bereits ein verhältnismäßig weit vorgeschrittenes Stadium darstellen. Es gelingt mittels kurzer Stöße erste lawinenartige Anfänge der Figuren festzuhalten und einiges über Vorgänge im Anfangsstadium herauszulesen. Die negative Figur beginnt lawinenförmig an bevorzugten Stellen der Kathode. Bei nebeneinander entstandenen Lawinen ist sehr gut die gegenseitige Abstoßung infolge gleicher Ladung zu beobachten. Die Entladung hat einen geschichteten Aufbau. Die positiven Figuren bilden sich später aus. Es sprechen jedoch bisher keinerlei Anzeichen für ein Hinwachsen der Fäden zur Anode. Die Kanäle verjüngen sich zur Anode hin und selbst bei kurzen Stoßzeiten endet kein einziger vor der Anode. Auch die Art der gegenseitigen Abstoßung der positiven Fäden spricht mehr für ein Vorwachsen von der Anode aus. Aus den Farbaufnahmen ergibt sich für die filmschwärzende Strahlung eine Wellenlänge unter 450 m.  相似文献   
5.
With the burgeoning complexity and capabilities of modern information appliances and services, user modelling is becoming an increasingly important research area. Simple user profiles already personalise many software products and consumer goods such as digital TV recorders and mobile phones. A user model should be easy to initialise, and it must adapt in the light of interaction with the user. In many cases, a large amount of training data is needed to generate a user model, and adaptation is equivalent to retraining the system. This paper briefly outlines the user modelling problem and work done at BTexact on an intelligent personal assistant (IPA) which incorporates a user profile. We go on to describe FILUM, a more flexible method of user modelling, and show its application to the telephone assistant and email assistant components of the IPA, with tests to illustrate its usefulness.  相似文献   
6.
7.
Underflow baffles have gained in popularity over the years as a viable mean to intercept floatables in Combined Sewer Overflows (CSOs). This choice was mainly justified by the extremely low capital cost (CAPEX) and operational cost (OPEX) of this solution, although the efficiency of underflow baffles has never been clearly proven. The only similar application to underflow baffles are scum boards in grit chambers and clarifier. However, the flow conditions at CSOs vary considerably from those in grit chambers and clarifier. For this reason, review of the behavior of floatables in a rapid flow is paramount. Only then can comprehensive design criteria for underflow baffles and overflow chambers be suggested. Pilot scale tests, performed in a 17 metres long basin at various flowrates, had already shown that a critical horizontal velocity for floatables (Vcr) may develop in the overflow chambers. In this follow up study, the fate of intercepted floatables was investigated. It appears from this latest data that permanent capture of floatables decreases rapidly with an increase in the horizontal velocity of the flow, no matter what the baffle depth. Baffle depth increases capture at lower velocities (0.17 m/s) but become irrelevant at higher velocities (0.61 m/s). This data suggests that capture efficiency of existing underflow baffles in overflow chambers can be, at best, very low whenever the horizontal velocity increases above 0.30 m/s or 1 ft/s.  相似文献   
8.
We present the results from a user study looking at the ability of observers to mentally integrate wind direction and magnitude over a vector field. The data set chosen for the study is an MM5 (PSU/NCAR Mesoscale Model) simulation of Hurricane Lili over the Gulf of Mexico as it approaches the southeastern United States. Nine observers participated in the study. This study investigates the effect of layering on the observer's ability to determine the magnitude and direction of a vector field. We found a tendency for observers to underestimate the magnitude of the vectors and a counter‐clockwise bias when determining the average direction of a vector field. We completed an additional study with two observers to try to uncover the source of the counter‐clockwise bias. These results have direct implications to atmospheric scientists, but may also be able to be applied to other fields that use 2D vector fields.  相似文献   
9.
Design science in information systems research   总被引:1,自引:1,他引:0  
  相似文献   
10.
Exchange and oxidation of C16O were investigated at 450°C on 18O-predosed Rh and Pt catalysts supported on A12O3, CeO2 and CeO2-Al2O3. In all cases, a rapid exchange of C16O with the surface can be observed. CO oxidation leads to C16O2, C16O18O and C18O2. Significant formation of C16O2 is due to the relatively high 16O coverage in reaction resulting from the C16O exchange and from an exchange between O surface species and 16O internal atoms. Hydrogen is also formed via a water-gas shift reaction (CO + surface OH) in higher proportion on CeO2-containing catalysts than on A12O3. Chlorine inhibits all the reactions (exchange, oxidation and WGS) and particularly the internal exchange.  相似文献   
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