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Caroline K. Brennan Marya Y. Ornelas Zi W. Yao Prof. Dr. Jennifer A. Prescher 《Chembiochem : a European journal of chemical biology》2021,22(16):2650-2654
Bioluminescent tools have been used for decades to image processes in complex tissues and preclinical models. However, few distinct probes are available to probe multicellular interactions. We and others are addressing this limitation by engineering new luciferases that can selectively process synthetic luciferin analogues. In this work, we explored naphthylamino luciferins as orthogonal bioluminescent probes. Three analogues were prepared using an optimized synthetic route. The luciferins were found to be robust emitters with native luciferase in vitro and in cellulo. We further screened the analogues against libraries of luciferase mutants to identify unique enzyme-substrate pairs. The new probes can be used in conjunction with existing bioluminescent tools for multi-component imaging. 相似文献
3.
Marya Wani Vishnupriya Raghavan Dolphy Abraham Virginia Kleist 《Information Systems Frontiers》2017,19(4):769-785
Travel websites are extensively used by travelers for their travel bookings, yet little is known about the hedonic effectiveness of such experiences for the customer. The end users of these websites are customers who may not always be sophisticated information system (IS) users. Further, traditional utility based measures of customer evaluation are dated with respect to the interactive nature of the technology in use and the hedonic benefits that may result from the use of the technology. The evaluation of IS needs to include hedonic measures facilitated by the interactive technology in addition to the traditional utility based measures. We propose and test an evaluation model for retail travel websites that combines the traditional utility based measures with hedonic measures which collectively create a more comprehensive measure for the IS evaluation of consumer focused websites. Thus rooted in theory, the model extends DeLone and McLean’s model of IS Success by adding the construct of User Experience. The model was tested on a sample of 255 customers of travel websites targeted at the Indian market. The results indicate that both utility based and hedonic measures are important factors for customer IS (travel website in this study) evaluation. The larger implications for theory and practice of IS evaluation are explained. 相似文献
4.
Evaluation of Crop Models for Simulating and Optimizing Deficit Irrigation Systems in Arid and Semi-arid Countries Under Climate Variability 总被引:2,自引:2,他引:0
Sebastian?KlossEmail author Raji?Pushpalatha Kefasi?J.?Kamoyo Niels?Schütze 《Water Resources Management》2012,26(4):997-1014
The variability of fresh water availability in arid and semi-arid countries poses a serious challenge to farmers to cope with
when depending on irrigation for crop growing. This has shifted the focus onto improving irrigation management and water productivity
(WP) through controlled deficit irrigation (DI). DI can be conceived as a strategy to deal with these challenges but more
knowledge on risks and chances of this strategy is urgently needed. The availability of simulation models that can reliably
predict crop yield under the influence of soil, atmosphere, irrigation, and agricultural management practices is a prerequisite
for deriving reliable and effective deficit irrigation strategies. In this context, this article discusses the performance
of the crop models CropWat, PILOTE, Daisy, and APSIM when being part of a stochastic simulation-based approach to improve
WP by focusing primarily on the impact of climate variability. The stochastic framework consists of: (i) a weather generator
for simulating regional impacts of climate variability; (ii) a tailor-made evolutionary optimization algorithm for optimal
irrigation scheduling with limited water supply; and (iii) the above mentioned models for simulating water transport and crop
growth in a sound manner. The results present stochastic crop water production functions (SCWPFs) that can be used as basic
tools for assessing the impact on the risk for the potential yield due to water stress and climate variability. Example simulations
from India, Malawi, France and Oman are presented and the suitability of these crop models to be employed in a framework for
optimizing WP is evaluated. 相似文献
5.
Using photo acoustic technique, the thermal properties of CdS thin films grown by spray pyrolysis are measured. Thermal diffusivity
and conductivity in these films decrease at least two orders compared with bulk. These results are compared with our study
on nano CdS and the other available literature. The comparison is good. The dependence of thermal diffusivity on the thickness
of the layer or the size of the particles on the glass substrate are analysed from the present measurement and discussed.
The dependence of thermal diffusivity on the thickness of the layer on the glass substrate is discussed. 相似文献
6.
A 2D Finite Element Model set up using the Arbitrary Lagrangian Eulerian (A.L.E) formulation proposed in Abaqus/Explicit (v6.4) is employed to predict serrated chip formation during cutting process. No artificial criterion is employed to create the chip or to initiate serrated chip formation. The sensitivity of serrated chip prediction to numerical and process parameters is analyzed in this paper. Experimental tests in orthogonal cutting conditions on machining of AISI-4140 with coated and uncoated cemented-carbide inserts were carried out to validate numerical results. They showed significant influence of cutting speed and rake angle on the serrated chip phenomena. The comparison between numerical and experimental results showed a good qualitative agreement and underlined the outstanding influence of the element dimensions employed in Finite Element Modeling (F.E.M.) tests. 相似文献
7.
Collective action and community ecology theories frame this study of longitudinal interorganizational networks in Croatia during the country's political transition. As time progresses toward political stability, grass-roots organizing activities shift through participation in new networks. Although engaged cross-sector communication was important in early stages of the transformation, homophilous partnering emerged as the system stabilized. System stability left room for organizations to exit the collective action network but with costs associated with centralized organizing. Over time, organizations embodied roles as ideological leaders, collective action network leaders, and within-sector network partners. We offer a unique contribution to community ecology and collective action theories with a communication-centered framework that emphasizes the nature of communication in interorganizational networks over a 4-year period. 相似文献
8.
F. Gratecap M. Girard S. Marya G. Racineux 《International Journal of Material Forming》2012,5(2):99-107
Surface striations with spacing equal to feed rate per rotation and banded structures in the weld nugget are some of the striking
features of friction stir welding. However, their formation is still subject to some debate. This study contributes to comprehend
their formation by evaluating the possible role played by the eccentricity of the tool during the welding of an aluminium
alloy and using a plasticine as its analogue. The eccentric movement is visualized to generate both surface and bulk striations
in plasticine. By voluntarily using non optimized welding conditions on aluminium, the material flow has been deduced and
confronted with direct visual observations through high speed camera on plasticine. In the non closed section of the weld,
two lobes each with thickness equal to feed rate per rotation were observed. First lobe corresponds to flow induced by the
pin and tests on plasticine showed constant volume displacement per rotation for a given tool eccentricity. The second lobe
is generated by material flow from under the shoulder back to the rear of the pin. The assembling or not of these two lobs
behind the pin can explain some of the characteristic patterns observed in the weld nugget such as onion rings, oxide dispersions
and cavities. 相似文献
9.
Igor Jerković Marina Rajić Zvonimir Marijanović Mate Bilić Stela Jokić 《分离科学与技术》2016,51(18):2925-2931
Supercritical (SC) CO2 extraction of dried Immortelle flowers was performed at different process parameters. The optimal extraction conditions related to the yield were determined by response surface methodology (yield 4.09% at 20 MPa and 52°C). The extracts were analyzed by GC-MS and tremetone derivatives dominated: bitalin A, 12-acetoxytremetone, gnaphaliol, 1-[2-(2-methyl-2,3-dihydroxypropyl)-2,3-dihydro-1-benzofuran-5-yl]ethanone, isobutyl bitalin A, and 1-[2-(acetylprop-1-en-2-yl)-3-hydroxy-2,3-dihydro-1-benzofuran-5-yl]ethanone. Striking differences were found among the essential oil and SC-CO2 extracts composition. The major oil compounds were γ-curcumene, α-pinene, β-selinene, α-selinene, and limonene. Mono- and sesquiterpene were found among minor constituents of the extracts. Neryl acetate was present in the extracts and essential oil. 相似文献
10.
Raji Vijayan Loganathan Chitra Sakayanathan Penislusshiyan 《International Journal of Food Properties》2018,21(1):674-684
Bioactive fraction of brown algae Sargassum wightii (SWE) was obtained using silica column chromatography and preparative Thin Layer Chromatography (TLC). FT-IR and LC–mass spectrometry ESI analysis revealed presence of various phlorotannins in the SWE. The IC50 value of SWE was found to be 59.91, 51.04, and 55.21 μg/ml for scavenging of 2,2-diphenyl-1-picrylhydrazyl, 2,2-azino-bis-(3-ethylbenzothiazoline-6-sulfonic acid), and Ferric Reducing Antioxidant Power assay, respectively. SWE inhibited angiotensin-I-converting enzyme (ACE) in mixed-type manner with IC50 value of 56.96 µg/ml and Ki value of 45 µg/ml. The dual function such as antioxidant and ACE inhibition of SWE warrants further study to understand the antihypertensive potential in vivo. 相似文献