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排序方式: 共有1341条查询结果,搜索用时 15 毫秒
1.
BS Kendrick BS Chang T Arakawa B Peterson TW Randolph MC Manning JF Carpenter 《Canadian Metallurgical Quarterly》1997,94(22):11917-11922
Understanding the mechanism for sucrose-induced protein stabilization is important in many diverse fields, ranging from biochemistry and environmental physiology to pharmaceutical science. Timasheff and Lee [Lee, J. C. & Timasheff, S. N. (1981) J. Biol. Chem. 256, 7193-7201] have established that thermodynamic stabilization of proteins by sucrose is due to preferential exclusion of the sugar from the protein's surface, which increases protein chemical potential. The current study measures the preferential exclusion of 1 M sucrose from a protein drug, recombinant interleukin 1 receptor antagonist (rhIL-1ra). It is proposed that the degree of preferential exclusion and increase in chemical potential are directly proportional to the protein surface area and that, hence, the system will favor the protein state with the smallest surface area. This mechanism explains the observed sucrose-induced restriction of rhIL-1ra conformational fluctuations, which were studied by hydrogen-deuterium exchange and cysteine reactivity measurements. Furthermore, infrared spectroscopy of rhlL-1ra suggested that a more ordered native conformation is induced by sucrose. Electron paramagnetic resonance spectroscopy demonstrated that in the presence of sucrose, spin-labeled cysteine 116 becomes more buried in the protein's interior and that the hydrodynamic diameter of the protein is reduced. The preferential exclusion of sucrose from the protein and the resulting shift in the equilibrium between protein states toward the most compact conformation account for sucrose-induced effects on rhIL-1ra. 相似文献
2.
T Noda M Arakawa T Takaya T Nagano K Kagawa H Miwa S Hirakawa 《Canadian Metallurgical Quarterly》1993,14(7):876-884
To evaluate right atrial (RA) contractile performance in patients with myocardial infarction, we validated a cineangiographic method of RA volume measurement, and investigated RA volume change in 'normal' individuals and patients with a previous myocardial infarction. Sixteen silicone rubber RA casts made from human cadavers were filmed in the postero-anterior and left lateral projections. The cast volumes calculated following Simpson's rule were in good agreement with those measured by water replacement (r = 0.992, P < 0.01). At cardiac catheterization, biplane RA cineangiography was performed in 19 'normal' individuals (N group), in 14 patients with a previous antero-septal infarction (AMI group) and in seven patients with a previous inferior infarction (IMI group). The RA volume-time curve was constructed at 20-40 ms intervals for one cardiac cycle. RA volume at the beginning of the atrial contraction (RAVd), which was defined as the 'preload' of the RA, tended to be larger in both the AMI and IMI groups compared with 'normal' individuals. The RA ejection volume was significantly larger in both the AMI (18.4 +/- 2.1 ml.m-2, P < 0.01) and IMI groups (19.4 +/- 2.8, P < 0.01) than in the N group (14.5 +/- 1.9), even for a comparable level of RAVd (range from 26 to 36 ml.m-2) (18.6 +/- 2.1, P < 0.01, 18.2 +/- 2.0, P < 0.01, 14.7 +/- 1.9, respectively). These results suggest that RA contraction increases in patients with myocardial infarction by increasing both the 'preload' and 'contractility' of the RA. 相似文献
3.
The effect of interface-roughness-related disorder on the electronic and optoelectronic properties of a quantum wire structure are studied. It is seen that the disorder causes strong localization in the quasi-one-dimensional system. While the electronic states are seriously perturbed, the density of states is not affected drastically. Optoelectronic properties as reflected in the interband transition related phenomenon are not found to suffer significant deterioration as a result of the disorder. However, the results suggest that intraband relaxation processes may be seriously affected because of electron (hole) states being localized in different regions of the wire 相似文献
4.
The oxidation of Fe(II) with dissolved molecular oxygen was studied in sulfuric acid solutions containing 0.2 mol . dm-3 FeSO4 at temperatures ranging from 343 to 363 K. In solutions of sulfuric acid above 0.4 mol . dm-3, the oxidation of Fe (II) was found to proceed through two parallel paths. In one path the reaction rate was proportional
to both [Fe2+]2 and po2, exhibiting an activation energy of 51.6 . kJ mol-1. In another path the reaction rate was proportional to [Fe2+]2, [SO4-], and po2 with an activation energy of 144.6 kJ . mol-1. A reaction mechanism in which the SO4- ions play an important role was proposed for the oxidation of Fe(II). In dilute solutions of sulfuric acid below 0.4 mol
. dm-3, the rate of the oxidation reaction was found to be proportional to both [Fe(II)]2 and Po2, and was also affected by [H+] and [SO2-
4]. The decrease in [H+] resulted in the increase of reaction rate. The discussion was further extended to the effect of Fe (III) on the oxidation
reaction of Fe (II). 相似文献
5.
A novel signal processing technique based on fuzzy rules is proposed for estimating nonstationary signals, such as image signals, contaminated with additive random noises. In this filter, fuzzy rules concerning the relationship between signal characteristics and filter design are utilized to set the filter parameters, taking the local characteristics of the signal into consideration. The fuzzy rules are found to be quite effective, since the rules to set the filter parameters are usually expressed in an ambiguous style. The high performance of this filter is demonstrated in noise reduction of a 1-D test signal and a natural image with various training signals 相似文献
6.
A Study of Shadowing on Indoor Visible-Light Wireless Communication Utilizing Plural White LED Lightings 总被引:3,自引:1,他引:2
Toshihiko Komine Shinichiro Haruyama Masao Nakagawa 《Wireless Personal Communications》2005,34(1-2):211-225
White LED is considered as a strong candidate for the future lighting technology. We have proposed an optical wireless communication
system that employs white LEDs for indoor wireless networks. In this system, LED is used not only as a lighting device, but
also as a communication device. The transmitter has large optical power and large emission characteristics to function as
lighting device. And the system has specific wireless channel impulse response differing from infrared wireless communication.
In this paper, we discuss about shadowing effect on the system utilizing plural LED lightings including the performance of
ISI based on the impulse response. We consider the downlink transmission based on TDMA and evaluate the shadowing effect caused
by pedestrians with computer simulation. When the shadowing often occurs at 800 Mb/s, the performance of outage call duration
rate and blocking rate are improved by using 3 LED lightings compared with 1 or 2 LED lightings. And, we show that the system
with the optimal number of the LED lighting is robust against shadowing and can accommodate more calls.
Toshihiko Komine was born in Shizuoka, Japan, on November 17, 1978. He received the B.E. and M.E. degrees in Information and Computer Science
from Keio University, Yokohama, Japan, in 2001 and 2003 respectively. He is currently studying for the Ph.D. degree at Department
of Information and Computer Science, Keio University. His current research interests are optical wireless communications and
LED communications.
Shinichiro Haruyama is a professor at Department of Information and Computer Science, Faculty of Science and Technology, Keio University, Yokohama,
Japan. He received an M.S. in engineering science from University of California at Berkeley in 1983 and a Ph.D. in computer
science from the University of Texas at Austin in 1990. He worked for Bell Laboratories of AT{&}T and Lucent Technologies,
U.S.A from 1991 to 1996, and for Sony Computer Science Laboratories, Inc. from 1998 to 2002. His research interests include
reconfigurable system, system design automation, wireless communication, and visible light communication.
Masao Nakagawa was born in Tokyo, Japan in 1946. He received the B.E., M.E. and Ph.D. degrees in electrical engineering from Keio University,
Yokohama, Japan, in 1969, 1971 and 1974 respectively. Since 1973, he has been with the Department of Electrical Engineering,
Keio University, where he is now a Professor. His research interests are in CDMA, consumer Communications, Mobile communications,
ITS (Intelligent Transport Systems), Wireless Home Networks, and Visible light Communication. He received 1989 IEEE Consumer
Electronics Society Paper Award, 1999-Fall Best Paper Award in IEEE VTC, IEICE Achievement Award in 2000, IEICE Fellow Award
in 2001. He was the executive committee chairman on International Symposium on Spread Spectrum Techniques and Applications
in 1992 and the technical program committee chairman of ISITA (International Symposium on Spread Spectrum Techniques and Applications)
in 1994. He is an editor of Wireless Personal Communications and was a guest editor of the special issues on “CDMA Networks
I, II, III and IV” published in IEEE JSAC in 1994 (I and II) and 1996 (III and IV). He chairs the Wireless Home Link sub-committee
in MMAC (Multimedia Mobile Access Communication Promotion Committee). 相似文献
7.
Yasushi Sobajima Kunihiro Mori Masahiro Tsukamoto Norimitsu Yoshida Masao Takahashi Hikaru Kobayashi Shuichi Nonomura 《Solar Energy Materials & Solar Cells》2005,85(2):240-187
Effects of cyanide (CN) treatment with hydrogenated amorphous silicon (a-Si:H) films have been investigated. The decrease of ΔV/V was observed in cyanide treated a-Si:H films and the successive thermal annealing at 200°C after CN treatment induced the further reduction of the ΔV/V. XPS spectra show the indirect evidence that the cyanide species is present within 10 nm from the hydrogenated amorphous silicon surface. The results of CN treatment with a-Si:H solar cells are demonstrated. 相似文献
8.
Recently, progress has been made in the Generalized Multi-Protocol Label Switching (GMPLS) and Automatic Switched Optical
Networks (ASON) standardizations. These technologies realize construction of large-scaled optical networks, interconnections
among single-domain Wavelength Division Multiplexing (WDM) networks, and direct communication over multi-domain WDM networks.
Meanwhile, it is known that the topology of the Internet exhibits the power-law attribute. Since the topology of the Internet,
which is constructed by interconnecting ASs, exhibits the power-law, there is a possibility that large-scale WDM networks,
which are constructed by interconnecting WDM networks, will also exhibit the power-law attribute. One of the structural properties
of a topology that adheres to the power-law is that most nodes have just a few links, although some have a tremendous number
of them. Another property is that the average distance between nodes is smaller than in a mesh-like network. A natural question
is how such a structural property performs in WDM networks. In this paper, we first investigate the property of the power-law
attribute of physical topologies for WDM networks. We compare the performance of WDM networks with mesh-like and power-law
topologies, and show that links connected to high-degree nodes are bottlenecks in power-law topologies. To relax this, we
introduce a concept of virtual fiber, which consists of two or more fibers, and propose its configuration method to utilize
wavelength resources more effectively. We compare performances of power-law networks with and without our method by computer
simulations. The results show that our method reduces the blocking probabilities by more than one order of magnitude. 相似文献
9.
10.