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1.
A method of positive identification of the specificity of polymerase chain reaction (PCR) product using internal oligonucleotide probe is introduced. The hybridization was done on the agarose gel which was dried after electrophoresis. Detection of the expression of T cell receptor a chain variable (TCR V alpha) genes on mRNA level was used as the experimental model. Twenty nine TCR V alpha gene subfamilies could be distinguished clearly in healthy human peripheral blood lymphocytes by this method. Positive identification of PCR product on dried agarose gel by internal oligonucleotide probe is relatively simple and less time consuming. 相似文献
2.
Designing a Production Line to Maximize Profit 总被引:1,自引:0,他引:1
The relationship between profitability and efficiency in a production line is discussed at length for the continuous production model. We prove that a solution which maximizes efficiency will not necessarily maximize profit. Several useful relationships between profitability and efficiency are developed which can greatly enhance computational efficiency. A solution procedure is developed which will determine the optimal number of work stations by maximizing an assumed profit function. An example is provided to illustrate these relationships and the entire solution procedure. 相似文献
3.
4.
Component commonality effects on inventory costs 总被引:5,自引:0,他引:5
In this paper we study the effects of increasing component commonality for a single-period model. A two-product, two-level configuration under a general component cost structure is considered. The economic implications of replacing different products' components by common components are analyzed. We develop optimal solutions for the Commonality and Non-Commonality (Basic) Models and provide bounds on the total savings resulting from using commonality. We demonstrate, under general and specific component cost structures, that some forms of commonality may not always be a preferred strategy. Furthermore, we present conditions under which commonality should not be used. Finally, an extension to the two-product multicomponent model is provided. 相似文献
5.
6.
This work presents experimental results on pickup velocity (critical velocity) measurements for a variety of particulate solids. The present experiments together with previously published experiments of a number of researchers encompass about 100 measurements of 24 materials for a wide range of particle sizes, shapes and densities. Based on the experimental results, three zones are defined by establishing simple relationships between the Reynolds and Archimedes numbers. The empirical relationships were further modified by taking into account the pipe diameter and particle shape (sphericity). The three-zone model was shown to reasonably correlate to Geldart's classification groups. 相似文献
7.
Meir Kalech Sarit Kraus Gal A. Kaminka Claudia V. Goldman 《Autonomous Agents and Multi-Agent Systems》2011,22(1):151-182
Voting is an essential mechanism that allows multiple agents to reach a joint decision. The joint decision, representing a
function over the preferences of all agents, is the winner among all possible (candidate) decisions. To compute the winning
candidate, previous work has typically assumed that voters send their complete set of preferences for computation, and in
fact this has been shown to be required in the worst case. However, in practice, it may be infeasible for all agents to send
a complete set of preferences due to communication limitations and willingness to keep as much information private as possible.
The goal of this paper is to empirically evaluate algorithms to reduce communication on various sets of experiments. Accordingly,
we propose an iterative algorithm that allows the agents to send only part of their preferences, incrementally. Experiments
with simulated and real-world data show that this algorithm results in an average of 35% savings in communications, while
guaranteeing that the actual winning candidate is revealed. A second algorithm applies a greedy heuristic to save up to 90%
of communications. While this heuristic algorithm cannot guarantee that a true winning candidate is found, we show that in
practice, close approximations are obtained. 相似文献
8.
9.
P. Ben-Ishai E. Sader Yu. Feldman I. Felner M. Weger 《Journal of Superconductivity and Novel Magnetism》2005,18(4):455-459
We present dielectric properties of ceramic anhydrous Na0.7CoO2 and the superconducting Na0.3CoO2·1.3H2O materials. The presence of water which induces superconductivity also may increase the dielectric constant (ε) of the hydrated material. This is consistent with the predicted relationship between the highε and the enhancement ofT
c in highT
c superconductors. The anhydrous sample is porous and the transport is due to some percolation via the pores. The porosity
is much higher for the hydrated material and the transport is ionic inside bulk water. 相似文献
10.
A calorimetric method is applied to determine the solar system performance by in-situ measured data. The heat store is interpreted as a calorimeter and the information on charge and discharge is extracted from the shape analysis of the heat store’s temperature profile. The method is applicable to any heating system which includes a heat store. In the present paper, this procedure is presented and applied to determine the solar gain for a large solar system for domestic hot water preparation and a solar combisystem. The uncertainty of this method is in the range of ±10%. The results obtained by the calorimetric method are compared to TRNSYS simulations. 相似文献