全文获取类型
收费全文 | 1378篇 |
免费 | 50篇 |
专业分类
电工技术 | 7篇 |
化学工业 | 156篇 |
金属工艺 | 7篇 |
机械仪表 | 19篇 |
建筑科学 | 12篇 |
能源动力 | 22篇 |
轻工业 | 105篇 |
水利工程 | 9篇 |
石油天然气 | 3篇 |
无线电 | 53篇 |
一般工业技术 | 100篇 |
冶金工业 | 860篇 |
原子能技术 | 5篇 |
自动化技术 | 70篇 |
出版年
2024年 | 4篇 |
2023年 | 7篇 |
2022年 | 18篇 |
2021年 | 28篇 |
2020年 | 14篇 |
2019年 | 15篇 |
2018年 | 25篇 |
2017年 | 26篇 |
2016年 | 27篇 |
2015年 | 26篇 |
2014年 | 16篇 |
2013年 | 44篇 |
2012年 | 35篇 |
2011年 | 51篇 |
2010年 | 39篇 |
2009年 | 38篇 |
2008年 | 25篇 |
2007年 | 19篇 |
2006年 | 19篇 |
2005年 | 20篇 |
2004年 | 10篇 |
2003年 | 9篇 |
2002年 | 7篇 |
2001年 | 9篇 |
2000年 | 5篇 |
1999年 | 35篇 |
1998年 | 265篇 |
1997年 | 159篇 |
1996年 | 106篇 |
1995年 | 62篇 |
1994年 | 39篇 |
1993年 | 47篇 |
1992年 | 7篇 |
1991年 | 11篇 |
1990年 | 9篇 |
1989年 | 11篇 |
1988年 | 13篇 |
1987年 | 7篇 |
1986年 | 4篇 |
1985年 | 6篇 |
1984年 | 2篇 |
1983年 | 6篇 |
1982年 | 2篇 |
1981年 | 9篇 |
1980年 | 11篇 |
1977年 | 20篇 |
1976年 | 53篇 |
1975年 | 2篇 |
1974年 | 2篇 |
1973年 | 2篇 |
排序方式: 共有1428条查询结果,搜索用时 15 毫秒
1.
We recently reported that epidermal immunization using the PowderJet particle delivery device with plasmid vector pcDNA1/EDIM6 encoding rotavirus VP6 of murine strain EDIM induced high levels of serum rotavirus IgG but failed to protect mice against EDIM infection (Choi, A. H., Knowlton, D. R., McNeal, M. M., and Ward, R. L. (1997) Virology 232, 129-138.). This was extended to determine whether pcDNA1/EDIM4 or pcDNA1/EDIM7, which encode either rotavirus VP4 or VP7, the rotavirus neutralization proteins, could also induce rotavirus-specific antibody responses and if these responses resulted in protection. Titers of rotavirus serum IgG increased with the first dose in mice immunized with pcDNA1/EDIM7, but little or no serum rotavirus IgG was detected in mice immunized with pcDNA1/EDIM4. In vitro assays with these plasmids in rabbit reticulocyte lysates showed that VP4 was expressed but the amount was considerably lower than VP6 or VP7. To improve expression of VP4 and induction of rotavirus-specific humoral responses, the coding region of VP4 was cloned into the high-expression plasmid WRG7054 as a fusion protein containing the 22-amino-acid secretory signal peptide of tissue plasminogen activator (tPA) at its N terminus. In vitro expression of tPA::VP4 was significantly higher than unmodified VP4, and mice inoculated with WRG7054/EDIM4 generated high titers of rotavirus IgG. The coding sequence of VP7 without the first 162 nucleotides was also cloned into WRG7054, but no difference was observed between titers of serum rotavirus IgG in mice immunized with this plasmid (WRG7054/EDIM7Delta1-162) and pcDNA1/EDIM7. The rotavirus-specific IgG titers in all immune sera were predominantly IgG1 indicating induction of Th 2-type responses. None of the mice immunized with any of the VP4 or VP7 plasmids developed serum or fecal rotavirus IgA or neutralizing antibody to EDIM. When immunized mice were challenged with EDIM virus, there was no significant reduction in viral shedding relative to unimmunized controls. Therefore epidermal immunization with VP4 or VP7 alone elicited rotavirus IgG responses but did not protect against homologous rotavirus challenge. 相似文献
2.
Two glial cell types surround olfactory axons and glomeruli in the olfactory bulb (OB) and may influence synapse development and regeneration. OB astrocytes resemble type-1 astrocytes, and OB ensheathing cells resemble non-myelinating Schwann cells. We have produced clonal OB astrocyte and ensheathing cell lines from rat neonatal and adult OB cultures by SV40 large T antigen transduction. These cell lines have been characterized by morphology, growth characteristics, immunophenotype, and ability to promote neurite outgrowth in vitro. Neonatal and adult ensheathing cell lines were found to support higher neurite outgrowth than OB astrocyte lines. Neonatal OB astrocyte lines were of two types, high and low outgrowth support. The low support astrocyte lines express J1 and a chondroitin sulfate-containing proteoglycan as do astrocytes encircling the neonatal glomeruli in vivo. The adult OB astrocyte cell lines supported lower levels of outgrowth than adult ensheathing cell lines. These results are consistent with a positive role for ensheathing cells in OB synapse regeneration, in vivo. Further, based on our results, we hypothesize that ensheathing cells and high-outgrowth astrocytes facilitate axon growth in vivo, while low outgrowth astrocytes inhibit axon growth and may facilitate glomerulus formation. 相似文献
3.
We have studied the inhibitory action of long- and short-chain fatty acids on hepatic glucose utilization in hepatocytes isolated from fasted rats. The rates of hepatic glucose phosphorylation and glycolysis were determined from the tritiated products of [2-3H] and [6-3H]glucose metabolism, respectively. The difference between these was taken as an estimate of the 'cycling' between glucose and glucose-6-phosphate. In the presence of 40 mM glucose this cycling was estimated at 0.68 mumol/min/g wet wt. Glucose phosphorylation was unaffected during palmitate and hexanoate oxidation to ketone bodies but glycolysis was inhibited. The rate of glucose cycling was increased during this phase to 1.25 mumol/min/g. Following the complete metabolism of the fatty acids, glycolysis was reinstated and cycling rates returned to control levels. Hepatic glucose cycling appears to be an important component of the glucose/fatty acid cycle. 相似文献
4.
The alarm lists presented to control center operators are usually difficult to interpret. The authors present an expert system that processes the alarm lists in Portuguese control centers. This system makes an intelligent synthesis of the available information and presents it in a flexible and structured way. It uses an original approach to deal with temporal reasoning and real-time constraints. The system incorporates an explanation module that enables its use as a tutor for novice operators 相似文献
5.
Infiltration systems are frequently used as an option to manage urban storm drainage. By reducing flows and volumes in downstream sewers or in surface waters, they decrease the overflows and make it possible to recharge groundwater. They come in various forms with different uses; therefore, their performance is diverse and integrates multiple aspects. Consequently, a multicriteria approach was developed in order to quantify the performance of these systems and to help in decision making problems. For that purpose, a list of performance indicators integrating technical, economical, environmental and social aspects was developed. The performances were defined with the help of a working group composed of engineers from different technical or strategic departments from Greater Lyon and researchers from different fields. The paper presents the last version of the performance indicators tested according to a set of quality requirements: availability of data, relevance, fidelity, precision, sensitivity/robustness. This critical review of the set of indicators has led us to redefine a certain number of indicators, identify numerous biases and allow putting forward general instructions for criterion or indicator construction. The last phase is to propose multicriteria decision aid methods; a procedure using ELECTRE methods should be used. 相似文献
6.
JD Haag LA Shepel BD Kolman DM Monson ME Benton KT Watts JL Waller CC Lopez-Guajardo DJ Samuelson MN Gould 《Canadian Metallurgical Quarterly》2003,63(18):5808-5812
It has previously been shown that the Copenhagen (COP) rat contains several genetic loci that contribute to its mammary tumor-resistant phenotype after 7,12-dimethylbenz(a)anthracene (DMBA) administration. One of these loci, mammary carcinoma susceptibility 1 (Mcs1), is located on the centromeric end of chromosome 2 and appears to act in a semidominant fashion. To confirm the existence and independent action of this locus and also aid in the identification of the physical location of the Mcs1 gene, congenic lines were generated by transferring the Mcs1 COP allele onto a Wistar Furth (WF) genetic background. Male carriers were genotyped using microsatellite markers spanning 20-30 cM of the Mcs1 locus. One of the congenic lines minimally retained the COP allele at D2Mit29 on the centromeric end of chromosome 2 and extended distally to D2Rat201. Heterozygous Mcs1 carrier rats were interbred, and the female offspring were treated with DMBA. The female rats from the Mcs1 congenic line that carried one or two COP alleles of the Mcs1 region had a significantly reduced (65 and 85%, respectively) tumor development (P < 0.001) compared with rats carrying zero COP alleles at this locus. A WF.COP-D2Mit29/D2Rat201 homozygous congenic strain derived at the N10 generation was treated with DMBA, and the COP homozygous rats developed 1.5 +/- 0.3 carcinomas/rat versus 6.3 +/- 0.5 in WF control rats (P < 0.0001). Fine mapping of this congenic interval using several recombinant lines identified three genetic loci within the Mcs1 congenic region that independently supported a tumor resistance phenotype. These genetic loci have been termed Mcs1a, Mcs1b, and Mcs1c. In rats for which each locus was homozygous for the COP allele, tumor development was reduced by approximately 60% compared with littermate controls. The identification of these independent loci within the Mcs1 COP allele provide a model of the genetic complexity of cancer. 相似文献
7.
MN Lowenthal 《Canadian Metallurgical Quarterly》1996,32(5):338-339
8.
9.
10.
Burns G. Leonard; Gomez Rapson; Walsh James A.; de Moura Marcela Alves 《Canadian Metallurgical Quarterly》2003,15(1):118
G. J. DuPaul (see record 2003-02033-012) offered two suggestions for additional research to understand the strong source effects reported by R. Gomez, G. L Burns, J. A. Walsh, and M. A. de Moura (see record 2003-02033-001) in attention-deficit/ hyperactivity disorder (ADHD) rating scales. The first suggestion was to determine whether the source effects represent mostly bias or accuracy. The second suggestion was to minimize source effects through the development of better ADHD rating scales. Because source effects can represent bias or accuracy, it is important to minimize the bias aspect through content validation procedures prior to attempts to determine whether source effects better reflect bias or accuracy. This comment offers various suggestions to reduce the bias in ADHD rating scales. (PsycINFO Database Record (c) 2010 APA, all rights reserved) 相似文献