Structural tuning of wide‐gap chalcopyrite CuGaSe2 thin films and highly efficient solar cells: differences from narrow‐gap Cu(In,Ga)Se2

Simultaneous realization of high values of open circuit voltage (V_oc), fill factor (FF), and energy conversion efficiency (η) in wide‐gap CuGaSe₂ (CGS) solar cells has long been one of the most challenging issues in the realm of chalcopyrite photovoltaics. In this communication, structural tuning of CGS thin films by means of controlling the amount of Se flux used during CGS film growth and improvements in solar cell performance (V_oc > 0.9 V, FF > 0.7, and η > 10%) are demonstrated. Systematic variations in CGS film properties with the Se flux and correlation with device properties are shown. The unique CGS thin‐film growth kinetics, which are different from narrow‐gap Cu(In,Ga)Se₂, are also presented and discussed. This development of double digit efficiency for CGS solar cells opens a new frontier for the broad application of a new class of chalcopyrite‐based devices. Copyright © 2014 John Wiley & Sons, Ltd.     

Towards Designing a Trusted Routing Solution in Mobile Ad Hoc Networks

Designing a trusted and secure routing solution in an untrustworthy scenario is always a challenging problem. Lack of physical security and low trust levels among nodes in an ad hoc network demands a secure end-to-end route free of any malicious entity. This is particularly challenging when malicious nodes collude with one another to disrupt the network operation. In this paper we have designed a secure routing solution to find an end-to-end route free of malicious nodes with collaborative effort from the neighbors. We have also extended the solution to secure the network against colluding malicious nodes, which, to the best of our knowledge, is the first such solution proposed. We have also proposed a framework for computing and distributing trusts that can be used with out trusted routing protocol. Our proposed framework is unique and different from the other schemes in that it tries to analyze the psychology of the attacker and quantifies the behavior in the computational model. Extensive simulation has been carried out to evaluate the design of our protocol. Partially funded by Department of Defense Award No. H98230-04-C-0460, Department of Transportation Project No. FL-26-7102-00 and National Science Foundation Grant Nos. ANI-0123950 and CCR-0196557. Tirthankar Ghosh is a PhD candidate in the Telecommunications and Information Technology Institute at Florida International University. His area of research is routing security and trust computation in wireless ad hoc and sensor networks. He received his Bachelor of Electrical Engineering from Jadavpur University, India and Masters in Computer Engineering from Florida International University. Dr. Niki Pissinou received her Ph.D. in Computer Science from the University of Southern California, her M.S. in Computer Science from the University of California at Riverside, and her B.S.I.S.E. in Industrial and Systems Engineering from The Ohio State University. She is currently a tenured professor and the director of the Telecommunication & Information Technology Institute at FIU. Previously Dr. Pissinou was a tenured faculty at the Center for Advanced Computer Studies at the University of Louisiana at Lafayette where she was also the director of the Telecommunication & Information & Technology Laboratory partially funded by NASA, and the co-director of the NOMAD: A Wireless and Nomadic Laboratory partially funded by NSF, and the Advanced Network Laboratory. Dr. Pissinou is active in the fields computer networks, information technology and distributed systems. Dr. Kami (Sam) Makki has earned his Ph.D. in Computer Science from the University of Queensland in Brisbane Australia, his Masters degree in Computer Science and Engineering from the University of New South Wales in Sydney Australia, and his Bachelor and Masters Degrees in Civil Engineering from the University of Tehran Iran. Before joining the department of Electrical Engineering and Computer Science at the University of Toledo he has held a number of academic positions and research appointments at the Queensland University of Technology in Brisbane, Royal Melbourne Institution of Technology in Melbourne and at The University of Queensland in Brisbane Australia. He is an active researcher in the fields of distributed systems, databases, mobile and wireless communications, and has more than 30 publications in peerreviewed journals and international proceedings. He has served as a chair and technical program committee member and reviewer for a number of IEEE and ACM sponsored technical conferences and has received a number of achievement awards.     

Antioxidant action of a lipophilic nitroxyl radical,cyclohexane-1-spiro-2′-(4′-oxyimidazolidine-1′-oxyl)-5′-spiro-1″-cyclohexane,against lipid peroxidation under hypoxic conditions

Nitroxyl radicals are known to act as radical scavenging antioxidants. In the present study, a lipophilic nitroxyl radical, cyclohexane-1-spiro-2′-(4′-oxyimidazolidine-1′-oxyl)-5′-spiro-1″-cyclohexane (nitroxyl radical I) was synthesized and its antioxidant capacity was assessed in comparison with a hydrophilic nitroxyl radical, 4-hydroxy-2,2,6,6-tetramethylpiperidine-N-oxyl (Tempol). Both nitroxyl radical I and Tempol inhibited methyl linoleate oxidation induced by free radicals, and the efficacy increased with decreasing partial pressure of oxygen, the effect being more pronounced for nitroxyl radical I than Tempol. Their hydroxylamines inhibited lipid peroxidation more effectively than their corresponding parent nitroxyl radicals. In liposomal membranes, a synergistic effect was observed in the combination of nitroxyl radical I with ascorbic acid, whereas only an additive effect was observed between Tempol and ascorbic acid. The present study suggests that nitroxyl radical I and its hydroxylamine may act as potent antioxidants, especially in combination with ascorbic acid under hypoxic conditions.     

Stimulation of THP-1 Macrophages with LPS Increased the Production of Osteopontin-Encapsulating Exosome

Osteopontin (OPN) mediates bone remodeling and tissue debridement. The OPN protein is cleaved, but it is unclear how full-length (FL)-OPN or its cleaved form perform their biological activities in target cells. We, therefore, performed the molecular characterization of OPN in exosomes (Exo). The Exo were isolated from lipopolysaccharide (LPS)-stimulated phorbol 12-myristate 13-acetate (PMA)-differentiated THP-1 macrophages. The Exo were also isolated from PMA-differentiated THP-1 macrophages. The Exo were identified using the qNano multiple analyzer (diameter 59–315 nm) and western blotting with a CD9 antibody. LPS-stimulated cells produced more particles than non-stimulated cells. The presence of the FL or the cleaved form of OPN was confirmed using western blot analysis. A mixture of FL and cleaved OPN was also measured using an ELISA system (Ud-OPN) and their presence in the Exo was confirmed. Ud/FL ratios became low after LPS stimulation, indicating the enhanced encapsulation of FL-OPN in the Exo by LPS. These findings suggest that LPS stimulation of human macrophages facilitates the synthesis of FL-OPN, which is cleaved in cells or the Exo after release. These findings indicate that Exo is a suitable vehicle to transfer OPN to the target cells.     

Determination of Carbohydrate‐Binding Preferences of Human Galectins with Carbohydrate Microarrays

Galectins are a class of carbohydrate‐binding proteins named for their galactose‐binding preference and are involved in a host of processes ranging from homeostasis of organisms to immune responses. As a first step towards correlating the carbohydrate‐binding preferences of the different galectins with their biological functions, we determined carbohydrate recognition fine‐specificities of galectins with the aid of carbohydrate microarrays. A focused set of oligosaccharides considered relevant to galectins was prepared by chemical synthesis. Structure–activity relationships for galectin–sugar interactions were determined, and these helped in the establishment of redundant and specific galectin actions by comparison of binding preferences. Distinct glycosylations on the basic lactosyl motifs proved to be key to galectin binding regulation—and therefore galectin action—as either high‐affinity ligands are produced or binding is blocked. High‐affinity ligands such as the blood group antigens that presumably mediate particular functions were identified.     

High Levels of the Cleaved Form of Galectin-9 and Osteopontin in the Plasma Are Associated with Inflammatory Markers That Reflect the Severity of COVID-19 Pneumonia

Numbers of patients with coronavirus disease 2019 (COVID-19) have increased rapidly worldwide. Plasma levels of full-length galectin-9 (FL-Gal9) and osteopontin (FL-OPN) as well as their truncated forms (Tr-Gal9, Ud-OPN, respectively), are representative inflammatory biomarkers. Here, we measured FL-Gal9, FL-OPN, Tr-Gal9, and Ud-OPN in 94 plasma samples obtained from 23 COVID-19-infected patients with mild clinical symptoms (CV), 25 COVID-19 patients associated with pneumonia (CP), and 14 patients with bacterial infection (ID). The four proteins were significantly elevated in the CP group when compared with healthy individuals. ROC analysis between the CV and CP groups showed that C-reactive protein had the highest ability to differentiate, followed by Tr-Gal9 and ferritin. Spearman’s correlation analysis showed that Tr-Gal9 and Ud-OPN but not FL-Gal9 and FL-OPN, had a significant association with laboratory markers for lung function, inflammation, coagulopathy, and kidney function in CP patients. CP patients treated with tocilizumab had reduced levels of FL-Gal9, Tr-Gal9, and Ud-OPN. It was suggested that OPN is cleaved by interleukin-6-dependent proteases. These findings suggest that the cleaved forms of OPN and galectin-9 can be used to monitor the severity of pathological inflammation and the therapeutic effects of tocilizumab in CP patients.     

Drivers and barriers for implementation of environmental strategies in manufacturing companies

In order for environmental strategies to come into effect in industry practice, they need to be implemented and applied in daily business routines. Based on a dedicated comprehensive international survey in product developing and manufacturing companies, this paper identifies major current drivers for implementing product life cycle oriented environmental strategies but also barriers and obstacles that need to be addressed. On this basis it provides a number of recommendations for manufacturing companies as well as policy makers to consider for a successful implementation of strategic environmental goals in manufacturing industry.     

Corrosion and protection of carbon steel in low enthalpy geothermal fluids. The case of SOUSAKI IN Greece

This experimental work is aimed at evaluating first, the effect of a solution simulating the geothermal fluid of the field in Sousaki, New Korinthia, on the corrosion of bare steel, and, second, the protective performance that certain combinations of organic coatings provide. This evaluation resulted from corrosion potential measurements, mass loss measurements, corrosion rate estimates by the Tafel method, and visual ranking of the degree of rusting and blistering. Six combinations of organic coatings were tested. Four of them consisted of water-based paints as primer, as well as intermediate and topcoat. Of the two remaining combinations, the first comprised a water-based primer, including dispersed tannin and a two-component epoxy resin topcoat, while the second consisted of a two-component acrylic primer, containing chromate pigments and the same epoxy resin topcoat as above. All coating combinations tested exhibited protective performance. The results of the water-based coatings were less satisfactory than those of the two combinations containing the epoxy resin topcoat.     

Assignment of most genes encoding major peroxisomal polypeptides to chromosomal band V of the asporogenic yeast Candida tropicalis

The peroxisomes of the asporogenic yeast Candida tropicalis contain about 20 major polypeptides (PXPs). We have isolated a number of genes encoding them; 11 POX genes encoded independent PXPs and three POY genes were likely to encode three other PXPs. To locate these genes on the chromosomes, chromosomes of C. tropicalis were separated by pulsed-field gel electrophoresis. Eight chromosomal bands were observed over the range of 1.0 Mbp (band 1) to 2.8 Mbp (band VIII); the genome size was estimated to be about 20 Mbp. Southern blot analysis showed that ten genes were on band V, three genes were on band IV, and the other gene was on band VI. Three genes gave hybridization signals of nearly equal intensity on two different chromosomal bands: POX6A and POX8B, on bands V and VII; and POX8A, on bands IV and VI. Ribosomal RNA genes also hybridized to two bands, VI and VII. Most genes assigned to only one band hybridized to two restriction fragments produced by either NotI or SfiI endonuclease. The results suggested that C. tropicalis was diploid and that restriction sites were conserved little between homologues. The three POX genes that were found on two chromosomal bands hybridized to not more than two restriction fragments, implying that the allelic genes were present on different chromosomal bands.