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排序方式: 共有223条查询结果,搜索用时 15 毫秒
1.
Jasmin Kuhn Philipp M. Klein Nader Al Danaf Joel Z. Nordin Sren Reinhard Dominik M. Loy Miriam Hhn Samir El Andaloussi Don C. Lamb Ernst Wagner Yoshitsugu Aoki Taavi Lehto Ulrich Lchelt 《Advanced functional materials》2019,29(48)
Phosphorodiamidate morpholino oligomers (PMOs) are oligonucleotide analogs that can be used for therapeutic modulation of pre‐mRNA splicing. Similar to other classes of nucleic acid‐based therapeutics, PMOs require delivery systems for efficient transport to the intracellular target sites. Here, artificial peptides based on the oligo(ethylenamino) acid succinyl‐tetraethylenpentamine (Stp), hydrophobic modifications, and an azide group are presented, which are used for strain‐promoted azide‐alkyne cycloaddition conjugation with splice‐switching PMOs. By systematically varying the lead structure and formulation, it is determined that the type of contained fatty acid and supramolecular assembly have a critical impact on the delivery efficacy. A compound containing linolenic acid with three cis double bonds exhibits the highest splice‐switching activity and significantly increases functional protein expression in pLuc/705 reporter cells in vitro and after local administration in vivo. Structural and mechanistic studies reveal that the lipopeptide PMO conjugates form nanoparticles, which accelerate cellular uptake and that the content of unsaturated fatty acids enhances endosomal escape. In an in vitro Duchenne muscular dystrophy exon skipping model using H2K‐mdx52 dystrophic skeletal myotubes, the highly potent PMO conjugates mediate significant splice‐switching at very low nanomolar concentrations. The presented aminoethylene‐lipopeptides are thus a promising platform for the generation of PMO‐therapeutics with a favorable activity/toxicity profile. 相似文献
2.
3.
F Balagué P Damidot M Nordin M Parnianpour M Waldburger 《Canadian Metallurgical Quarterly》1993,18(9):1199-1205
Our surveys have shown lifetime prevalence of L.BP. over 30% among schoolchildren. The purpose of this study was to evaluate the relationship between back and isokinetic trunk strength, anthropometric parameters, and sports activities. One hundred and seventeen healthy children aged 10-16 years were included. All these volunteers had semi-structured interview, anthropometric and dynamic strength measurements. Lifetime prevalence of back pain was 44.5% and point prevalence was 13%. In this cross-sectional study, anthropometric and strength profiles were significantly related to age and gender. Non specific low back pain was not correlated to trunk muscle strength and/or sports activities. 相似文献
4.
Mohajeri S Aziz HA Zahed MA Mohajeri L Bashir MJ Aziz SQ Adlan MN Isa MH 《Water science and technology》2011,64(8):1652-1660
Landfill leachate is one of the most recalcitrant wastes for biotreatment and can be considered a potential source of contamination to surface and groundwater ecosystems. In the present study, Fenton oxidation was employed for degradation of stabilized landfill leachate. Response surface methodology was applied to analyze, model and optimize the process parameters, i.e. pH and reaction time as well as the initial concentrations of hydrogen peroxide and ferrous ion. Analysis of variance showed that good coefficients of determination were obtained (R2 > 0.99), thus ensuring satisfactory agreement of the second-order regression model with the experimental data. The results indicated that, pH and its quadratic effects were the main factors influencing Fenton oxidation. Furthermore, antagonistic effects between pH and other variables were observed. The optimum H2O2 concentration, Fe(II) concentration, pH and reaction time were 0.033 mol/L, 0.011 mol/L, 3 and 145 min, respectively, with 58.3% COD, 79.0% color and 82.1% iron removals. 相似文献
5.
Liquid crystal-on-silicon implementation of the partial pixel three-dimensional display architecture
Nordin GP Kulick JH Lindquist RG Nasiatka PJ Jones MW Friends M Kowel ST 《Applied optics》1995,34(19):3756-3763
We report the implementation of a liquid crystal-on-silicon, three-dimensional (3-D) diffractive display based on the partial pixel architecture. The display generates multiple stereoscopic images that are perceived as a static 3-D scene with one-dimensional motion parallax in a manner that is functionally equivalent to a holographic stereogram. The images are created with diffraction gratings formed in a thin liquid crystal layer by fringing electric fields from transparent indium tin oxide interdigitated electrodes. The electrodes are controlled by an external drive signal that permits the 3-D scene to be turned on and off. The display has a contrast ratio of 5.8, which is limited principally by optical scatter caused by extraneous fringing fields. These scatter sources can be readily eliminated. The display reported herein is the first step toward a real-time partial pixel architecture display in which large numbers of dynamic gratings are independently controlled by underlying silicon drive circuitry. 相似文献
6.
Noraini Nordin Samikannu Kanagesan Nur Rizi Zamberi Swee Keong Yeap Nadiah Abu Subramani Tamilselvan Mansor Hashim Noorjahan Banu Alitheen 《IET nanobiotechnology / IET》2017,11(3):343
In this study, nanocrystalline magnesium zinc ferrite nanoparticles were successfully prepared by a simple sol–gel method using copper nitrate and ferric nitrate as raw materials. The calcined samples were characterised by differential thermal analysis/thermogravimetric analysis, Fourier transform infrared spectroscopy and X‐ray diffraction. Transmission electron microscopy revealed that the average particle size of the calcined sample was in a range of 17–41 nm with an average of 29 nm and has spherical size. A cytotoxicity test was performed on human breast cancer cells (MDA MB‐231) and (MCF‐7) at various concentrations starting from (0 µg/ml) to (800 µg/ml). The sample possessed a mild toxic effect toward MDA MB‐231 and MCF‐7 after being examined with MTT (3‐[4, 5‐dimethylthiazol‐2‐yl]‐2, 5 diphenyltetrazolium bromide) assay for up to 72 h of incubation. Higher reduction of cells viability was observed as the concentration of sample was increased in MDA MB‐231 cell line than in MCF‐7. Therefore, further cytotoxicity tests were performed on MDA MB‐231 cell line.Inspec keywords: sol‐gel processing, nanoparticles, nanofabrication, magnesium compounds, zinc compounds, toxicology, biological organs, cancer, cellular biophysics, nanomedicine, calcination, differential thermal analysis, Fourier transform infrared spectra, X‐ray diffraction, transmission electron microscopy, particle size, organic compoundsOther keywords: sol‐gel method, cytotoxic effects, breast cancer cell line, MDA MB‐231 in vitro, nanocrystalline magnesium zinc ferrite nanoparticles, copper nitrate, ferric nitrate, raw materials, calcined samples, differential thermal analysis, thermogravimetric analysis, Fourier transform infrared spectroscopy, X‐ray diffraction, transmission electron microscopy, average particle size, cytotoxicity testing, human breast cancer cells, mild toxic effect, 3‐[4,5‐dimethylthiazol‐2‐yl]‐2,5 diphenyltetrazolium bromide) assay, cell viability, MCF‐7, MDA MB‐231 cell line, size 17 nm to 41 nm 相似文献
7.
Moslem Yousefi Rasul Enayatifar Amer Nordin Darus Abdul Hanan Abdullah 《International Communications in Heat and Mass Transfer》2012
This paper presents a robust, efficient and parameter-setting-free evolutionary approach for the optimal design of compact heat exchangers. A learning automata based particle swarm optimization (LAPSO) is developed for optimization task. Seven design parameters, including discreet and continuous ones, are considered as optimization variables. To make the constraint handling straightforward, a self-adaptive penalty function method is employed. The efficiency and the accuracy of the proposed method are demonstrated through two illustrative examples that include three objectives, namely minimum total annual cost, minimum weight and minimum number of entropy generation units. Numerical results indicate that the presented approach generates the optimum configuration with higher accuracy and a higher success rate when compared with genetic algorithms (GAs) and particle swarm optimization (PSO). 相似文献
8.
A comprehensive review on theoretical framework‐based electric vehicle consumer adoption research
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Nadia Adnan Shahrina Md Nordin Imran Rahman Pandian M. Vasant Amir Noor 《国际能源研究杂志》2017,41(3):317-335
Green vehicles, such as electric vehicles (EVs), are getting noteworthy popularity among consumers worldwide. The purpose of this paper is to establish EVs as a feasible long‐term solution for the future of technology in the vehicle industry, which can decrease the current dependency on fossil fuels and also decrease greenhouse gas (GHG) emissions. As a part of long‐term benefits, the adoption of EVs gives environmentally friendly innovation to society. Despite positive environmental implications, the total number of EVs in usage is still inadequate. One of the major causes of this insubstantial adoption of EVs is largely dependent on the perceptions of consumers regarding EVs. However, this particular research study offers an inclusive outline on the existing hurdles for consumer adoption of EVs as well as a framework of the theoretical standpoints that were developed for the adoption behaviour, in addition to considering consumer intentions in the direction of EVs. In this particular study, the researcher found that the literature regarding EV adoption tried to address only the diffusion method of EVs. Whereas this study highlights consumer innovations, which provides a wide insight on consumer emotions to overlook the major aspect in consumer EVs' adoption research. The theme of this particular literature can be implemented in order to better understand the consumers' emotions and behaviour towards the adoption of EVs. The scholars further stated that there is a possible cause for more recent developments within the technological adoption part that can assist to be a standard for upcoming developments. For the last few years, knowledge regarding the problems surrounding the adoption and diffusion of EVs has gained less attention. This study expands this line of research by focusing on making a chance for developing the theoretical frameworks in terms of adding emotions in a psychological perspective where consumer behaviour and ethics are considered. Copyright © 2016 John Wiley & Sons, Ltd. 相似文献
9.
The corrosion resistance of different stainless steel grades have been studied as regards localized attack under service conditions. Test racks with welded samples with artificially applied crevices were inserted in three different flash chambers. Materials used for splash plates, brine piping and. condenser tubes were also tested.Beinge a pilot plant, various process conditions were tested. Hence, the materials have been exposed to different corrosive conditions, including stagnant seawater during shut-down periods.The results show that stainless steels of type 316L and higher alloyed exhibit good corrosion resistance, even in brine at high temperature viz pipes from the brine heater. 相似文献
10.
Rinku Saran Muhammad N. Nordin Richard J. Curry 《Advanced functional materials》2013,23(33):4149-4155
Hybrid PbS nanocrystal/C60 fullerite photodetectors are fabricated using a simple one‐step drop casting procedure onto pre‐patterned interdigitated electrodes. The devices exhibit a broad spectral response from the near UV through to the near infrared yielding a detectivity, D*, of above 1010 Jones from 400 nm to ≈1050 nm. The ability to further extend the spectral response to wavelengths ≈1350 nm in the near infrared via tuning of the PbS nanocrystal diameter is also demonstrated. The dynamic responses of the devices are presented, exhibiting a fast photocurrent rise time (<40 ns) followed by a long bi‐exponential decay with characteristic lifetimes of τ1 = 5.3 μs ± 0.1 μs and τ2 = 37.8 μs ± 0.7 μs. These devices, which have a detectivity approaching that of commercial detectors, a broader spectral response, and a fast rise time, offer an attractive low‐cost solution for large‐area broadband photodetectors. 相似文献