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1.
Wijaya A Hermann A Abriouel H Specht I Yousif NM Holzapfel WH Franz CM 《Journal of food protection》2004,67(12):2772-2778
Enterococcus faecium strain FAIR-E 345 isolated from food was shown to possess bile salt hydrolase (Bsh) activity in a plate screening assay and by high-performance liquid chromatography analysis. The bsh gene was cloned and sequenced. DNA sequence analysis revealed that it encoded a protein of 324 amino acids, with pI 4.877. A bsh gene probe was prepared from the cloned bsh gene and was used for probing plasmid and total genomic DNA of Bsh-positive enterococci isolated from food to determine the genomic location of their bsh genes. This probe was able to detect the bsh gene among total genomic DNA preparations but not from plasmid preparations of 10 plasmid-bearing Enterococcus strains. However, the probe could detect the bsh gene from total genomic DNA preparations of 12 Enterococcus strains that did not contain detectable plasmid DNA. In no cases did the probe hybridize with plasmid DNA preparations, suggesting that the bsh gene among enterococci is probably generally chromosomally encoded. This presumptive chromosomal location of bsh genes among food enterococci suggests that transfer of this trait by conjugative plasmids is unlikely. 相似文献
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AbstractData mining techniques have been successfully utilized in different applications of significant fields, including medical research. With the wealth of data available within the health-care systems, there is a lack of practical analysis tools to discover hidden relationships and trends in data. The complexity of medical data that is unfavorable for most models is a considerable challenge in prediction. The ability of a model to perform accurately and efficiently in disease diagnosis is extremely significant. Thus, the model must be selected to fit the data better, such that the learning from previous data is most efficient, and the diagnosis of the disease is highly accurate. This work is motivated by the limited number of regression analysis tools for multivariate counts in the literature. We propose two regression models for count data based on flexible distributions, namely, the multinomial Beta-Liouville and multinomial scaled Dirichlet, and evaluated the proposed models in the problem of disease diagnosis. The performance is evaluated based on the accuracy of the prediction which depends on the nature and complexity of the dataset. Our results show the efficiency of the two proposed regression models where the prediction performance of both models is competitive to other previously used regression models for count data and to the best results in the literature. 相似文献
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Nuha M.K. Yousif Melanie Huch Tobias Schuster Gyu-Sung Cho Hamid A. Dirar Wilhelm H. Holzapfel Charles M.A.P. Franz 《Food microbiology》2010
The diversity of lactic acid bacteria associated with Hussuwa fermentation, a Sudanese fermented sorghum food, was studied using a polyphasic taxonomical approach. Predominant strains could be well characterised based on a combination of phenotypic tests and genotypic methods such as ARDRA, rep-PCR and RAPD-PCR, as well as 16S rRNA gene sequencing of representative strains. Thus, the majority (128 of 220, 58.3%) of strains exhibited phenotypic properties typical of heterofermentative lactobacilli and of these, 100 strains were characterised more closely using the genotyping methods. The majority (97/100) strains could be characterised as Lactobacillus fermentum strains. Seventy-two of 220 strains (32.7%) showed phenotypic properties that are characteristic of pediococci. Of 41 selected strains investigated by genotyping techniques, 38 (92.7%) could be characterised as Pediococcus acidilactici strains, while three (7.3%) could be characterised as Pediococcus pentosaceus strains. The Hussuwa fermentation thus appears to be dominated by L. fermentum strains and P. acidilactici strains. For this reason, we selected representative and predominant strains as potential starter cultures for Hussuwa fermentation. These strains, L. fermentum strains BFE 2442 and BFE 2282 and P. acidilactici strain BFE 2300, were shown on the basis of RAPD-PCR fingerprinting to predominate in a model fermentation when used as starter cultures inoculated at 1 × 106 CFU/g and to lower the pH of the fermentation to below pH 4.0 within 48 h. These cultures should be studied for further development as starter preparations in pilot scale studies in actual field fermentations. 相似文献
4.
Nuha Y. Al-Attabi Raju Adhikari Peter Cass Mark Bown François Malherbe 《Materials Science & Technology》2019,35(4):462-468
Composites of silver nanowires (AgNWs) and polyurethane (PU) were prepared and their properties evaluated for potential applications as electrically conducting materials. Polyurethanes were chosen as the base substrate because of their inherent biocompatibility, biostability, excellent processability and good mechanical properties. The composites were prepared with varying content of AgNW by a solvent casting method. The electrical conductivity of the resulting composites was assessed using a 4-probe conductivity method and a maximum conductivity of 352.6?S?cm–1 was achieved with 25% AgNW loading. The composite films were then characterised by various techniques to investigate the structure–property relationships. The above-mentioned analyses showed that the composite films retained the tensile and thermal properties of the parent PU while showing excellent conductivity. 相似文献
5.
Nuha Salem 《Polymer》2005,46(19):8573-8581
Reversible addition-fragmentation chain transfer (RAFT) polymerizations were performed in the presence of organically modified clays and successfully prepared polystyrene-, poly(methyl methacrylate)-, and poly(n-butyl acrylate)-layered silicate nanocomposites. The polymers had well-defined molecular weights and low polydispersities, as expected from RAFT polymerizations. The morphology of polystyrene-, and poly(n-butyl acrylate)-nanocomposites were found to be exfoliated using montmorillonite modified with N,N-dimethyl-n-hexadecyl-(4-vinylbenzyl) ammonium (MMT-VB16). In the case of PMMA nanocomposite, the structure was a mixture of intercalated and exfoliated when MMT-VB16 was used, while the use of montmorillonite modified with 2-methacryloyloxyethyl-hexadecyldimethyl ammonium (MMT-MA16) resulted in exfoliation. 相似文献
6.
Nuha Ahmad Dsouki Maurício Pereira de Lima Roseli Corazzini Thaís Moura Gáscon Ligia Ajaime Azzalis Virgínia Berlanga Campos Junqueira David Feder Fernando Luiz Affonso Fonseca 《Journal of materials science. Materials in medicine》2014,25(5):1301-1305
The use of metal devices in medical application is increasing but it remains incompletely understood the physiological effects of component degradation. Niobium (Nb) alloys have already been investigated in the 1980’s and recent studies demonstrated the potential of Nb as an implant material. The purpose of this study was to determine cytotoxic, hematologic and histologic effects of niobium in Swiss mice. Animals were treated with a single dose of 3 % niobium oxide (Nb2O5) diluted in PBS, i.p. Cytotoxic assay, hematologic and histologic evaluation were done 3, 7 and 12 days after niobium treatment. Data have shown increased number of cells after niobium treatment, but there was no difference in cell viability. Furthermore, it was not observed hematological modification 3, 7 or 12 days after niobium treatment. Despite the fact that animals treated with niobium for 3 and 7 days showed mild degeneration in hepatocytes, mice kept alive for 12 days showed liver cells regeneration. Our results suggested that niobium cytotoxicity was not progressive because 12 days after treatment there was an evident liver regeneration. Data obtained indicated that niobium may be promising alternatives to biomedical applications. 相似文献
7.
Channel‐based radio‐frequency fingerprinting such as a channel impulse response (CIR), channel transfer function (CTF), and frequency coherence function (FCF) have been recently proposed to improve the accuracy at the physical layer; however, their empirical performance, advantages, and limitations have not been well reported. This paper provides a comprehensive empirical performance evaluation of RF location fingerprinting, focusing on a comparison of received‐signal strength, CIR‐, CTF‐, and FCF‐based fingerprinting using the weighted k‐nearest neighbor pattern recognition technique. Frequency domain channel measurements in the IEEE 802.11 band taken on a university campus were used to evaluate the accuracy of the fingerprinting types and their robustness to human‐induced motion perturbations of the channel. The localization performance was analyzed, and the results are described using the spatial and temporal radio propagation characteristics. In particular, we introduce the coherence region to explain the spatial properties and investigate the impact of the Doppler spread in time‐varying channels on the time coherence of RF fingerprint structures. 相似文献
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Nuha Zamzami Rua Alsuroji Oboh Eromonsele Nizar Bouguila 《Computational Intelligence》2020,36(2):459-485
This paper proposes an unsupervised algorithm for learning a finite mixture of scaled Dirichlet distributions. Parameters estimation is based on the maximum likelihood approach, and the minimum message length (MML) criterion is proposed for selecting the optimal number of components. This research work is motivated by the flexibility issues of the Dirichlet distribution, the widely used model for multivariate proportional data, which has prompted a number of scholars to search for generalizations of the Dirichlet. By introducing the extra parameters of the scaled Dirichlet, several useful statistical models could be obtained. Experimental results are presented using both synthetic and real datasets. Moreover, challenging real-world applications are empirically investigated to evaluate the efficiency of our proposed statistical framework. 相似文献
10.
Characterization of the 3Dimension optical,geometrical, and mechanical profiles of iPP fiber with necking deformation 下载免费PDF全文
We present a method for evaluating the 3D refractive indices and 3D true stress and/or 3D true strain profiles of “isotactic polypropylene iPP” fibers during necking deformation. Observing the changes in geometrical shape during the deformation process is necessary to understand the mechanical performance of iPP fibers. 3D geometric shape profile and actual stress and strain profiles were measured for iPP fibers during the propagation of neck deformation. These measurements were performed with the aid of an in‐situ opto‐ mechanical device to dynamically characterize different properties of fibers at different strain rates. A software image analysis program was used to calculate the 3D opto‐mechanical properties of iPP fibers. The obtained results show that the used dynamic stretching device can be easily used to monitor the deformation process with high accuracy. The effective stress and strain can be determined from the filaments profile. For illustration microinterferograms are given. 相似文献