Preparation and evaluation of Baicalin-loaded cationic solid lipid nanoparticles conjugated with OX26 for improved delivery across the BBB

Purpose: A novel brain targeting drug delivery system based on OX26 antibody conjugation on PEGylated cationic solid lipid nanoparticles (OX26-PEG-CSLN) was prepared.

Methods: The Baicalin-loaded PEGylated cationic solid lipid nanoparticles modified by OX26 antibody (OX26-PEG-CSLN) were prepared by emulsion evaporation–solidification at low temperature method. The immune-gold labeled OX26-PEG-CSLN was visualized by transmission electron microscopy. The mean diameter and zeta potential of OX26-PEG-CSLN, PEG-CSLN and CSLN were determined using a Zetasizer. The entrapment efficiency of OX26-PEG-CSLN, PEG-CSLN and CSLN was determined by ultrafiltration centrifugation method. And the solid-state characterization of OX26-PEG-CSLN and CSLN were analyzed by X-ray. Pharmacokinetics studies were conducted by in vivo microdialysis in rat cerebrospinal fluid.

Results: The results showed that the OX26-PEG-CSLN, PEG-CSLN and CSLN had average diameters of 47.68?±?1.65, 27.20?±?1.70 and 33.89?±?5.74?nm, Zeta potentials of ?0.533?±?0.115?mV, 11.200?±?0.500?mV and 11.080?±?1.170?mV and entrapment efficiencies of 83.03?±?0.01%, 92.90?±?3.50% and 97.83?±?0.19%, respectively. In the pharmacokinetics studies, the AUC value of OX26-PEG-CSLN was11.08-fold higher than that of the Baicalin solution (SOL) (p?p?>?0.05); the C_max value of OX26-PEG-CSLN was 7.88-fold higher than that of SOL (p?p?Conclusion: These results demonstrated OX26-PEG-CSLN could be a promising carrier to deliver drugs across the BBB for the treatment of brain diseases.     

Construction of recombinants Pseudomonas putida BO14 and Escherichia coli QEFCA8 for ferulic acid biotransformation to vanillin

Recombinants Pseudomonas putida BO14 and Escherichia coli QEFCA8 capable of ferulic acid biotransformation to vanillin were constructed using homologous recombination and a PCR based cloning strategy, respectively. In the liquid culture of P. putida BO14, 26.81+/-2.30 microg vanillin ml(-1) of culture filtrate was detected. In the case of recombinant E. coli QEFCA8, 19.37+/-1.95 microg vanillin ml(-1) of culture filtrate was detected. Results indicate that the strains could be useful for the biotechnological production of vanillin, a very important flavoring substance.     

Reactive flow in silicon electrodes assisted by the insertion of lithium

In the search for high-energy density materials for Li-ion batteries, silicon has emerged as a promising candidate for anodes due to its ability to absorb a large number of Li atoms. Lithiation of Si leads to large deformation and concurrent changes in its mechanical properties, from a brittle material in its pure form to a material that can sustain large inelastic deformation in the lithiated form. These remarkable changes in behavior pose a challenge to theoretical treatment of the material properties. Here, we provide a detailed picture of the origin of changes in the mechanical properties, based on first-principles calculations of the atomic-scale structural and electronic properties in a model amorphous silicon (a-Si) structure. We regard the reactive flow of lithiated silicon as a nonequilibrium process consisting of concurrent Li insertion driven by unbalanced chemical potential and flow driven by deviatoric stress. The reaction enables the material to flow at a lower level of stress. Our theoretical model is in excellent quantitative agreement with experimental measurements of lithiation-induced stress on a Si thin film.     

Level and degradation of Deepwater Horizon spilled oil in coastal marsh sediments and pore-water

This research investigates the level and degradation of oil at ten selected Gulf saltmarsh sites months after the 2010 BP Macondo-1 well oil spill. Very high levels (10-28%) of organic carbon within the heavily oiled sediments are clearly distinguished from those in pristine sediments (<3%). Dissolved organic carbon in contaminated pore-waters, ranging up to hundreds of mg/kg, are 1 to 2 orders of magnitude higher than those at pristine sites. Heavily oiled sediments are characterized by very high sulfide concentrations (up to 80 mg/kg) and abundance of sulfate reducing bacteria. Geochemical biomarkers and stable carbon isotope analyses fingerprint the presence of oils in sediments. Ratios of selected parameters calculated from the gas chromatograph spectra are in a remarkable narrow range among spilled oils and initial BP crude. At oiled sites dominated by C(4) plants, δ(13)C values of sediments (-20.8 ± 2.0‰) have been shifted significantly lower compared to marsh plants (-14.8 ± 0.6‰) due to the inflow of isotopically lighter oil (-27 ± 0.2‰). Our results show that (1) lighter compounds of oil are quickly degraded by microbes while the heavier fractions of oil still remain and (2) higher inputs of organic matter from the oil spill enhance the key microbial processes associated with sulfate reducing bacteria.     

Effects of Coating Materials on the Machinability of a Nickel Base,C-263, Alloy

PVD coated (TiN/TiCN/TiN, TiAIN and TiZrN) and uncoated carbide tools were used to machine a nickel base, C-263, alloy at high-speed conditions. The test results show that the multiple TiN/TiCN/TiN coated inserts gave the best overall performance in terms of tool life when machining at cutting speeds up to 68 m min and at depths of cut of 0.635 mm, 1.25 mm and 2.54 mm. All the tool grades tested gave fairly uniform surface roughness (Ra) values, below the rejection criterion, at lower speed conditions. The TiZrN coated inserts gave the lowest component forces when machining at lower cutting speed conditions while the TiA/N coated inserts gave the lowest component forces when machining at a higher speed of 68 m min^?1 and depth of cut of 1.25 mm. This tool performance can generally be attributed to the difference in their ability to provide effective lubrication at the cutting zone, thermal conductivity of the coating materials as well as the cutting conditions employed. The uncoated carbide tools generally encountered more severe crater wear, chipping/fracture of the cutting edges as well as pronounced notching during machining. This is due to their inability to provide effective lubrication at the cutting zone, thus impeding the gliding motion of the chips along the rake and flank faces respectively, thus accelerating flank wear. Analysis of the worn tool edges revealed adhesion of a compact “fin-shaped” structure of hardened burrs with saw-tooth like edges. This generally alters the initial geometry of the cutting edge, consequently resulting to poor surface finish with prolonged machining.     

Gaseous fuel production from fungal lignocellulose degradation

Anaerobic biodegradation of lignocellulose from elephant grass by cellulolytic fungi was carried out at optimum operational conditions: reactor volume of 50 cm³, slurry concentration of 2:5 w/v lignocellulose (g): water (cm³), and degradation temperature of 33 °C. Four cellulolytic fungi and a bacterium, which were harnessed from the air, were isolated in pure form and identified. Two species, Curvularia and Penicillium, were responsible for the biodegradation of lignocellulosic material, to yield biogas. The other two, Fusarium species and Aspergillus niger, including the bacterium, gave no gaseous products. Three gaseous products, namely methane, propane and carbon dioxide, were identified and measured. The rates of formation of these products were found to be first order with respect to the concentration of lignocellulose and the concentration of the microorganism, respectively. The distribution of the gaseous products suggests that the mechanism involves reductive decomposition of the cellulose monomeric unit, by the microorganism, to the gaseous products formed. Major heavier hydrocarbons produced in the degradation were also identified as mainly saturated polycyclic hydrocarbons, probably formed from the reductive degradation of the polyaromatic portions of the lignocellulose.     

The quasi-equilibrium response of MOS structures: Quasi-static factor

The dynamic response of a MOS structure driven into a non-equilibrium behaviour by a voltage ramp is presented. In contrast to Khun's quasi-static technique it is shown that any ramp-driven MOS structure has some degree of non-equilibrium. A quasi staticity factor μ_AK which serves as a measure of the degree of quasi-equilibrium, has been introduced for the first time. The mathematical model presented in the paper allows a better explanation of the experimental recordings. It is shown that this model could be used to analyse the various features of the response of the structure and that such physical parameters as the generation-rate, trap activation energy, and the effective capture constants could be obtained.     

SOLVENT DEMETALLIZATION OF ATMOSPHERIC AND VACUUM RESIDUES

The effectiveness of various solvents for the removal of heavy metals, particularly vanadium and nickel via the removal of asphaltene from atmospheric and vacuum petroleum residues are presented The solvent deasphalting was carried out at 29§C The solvents used in this investigation were pentane, hexane, heptane, petroleum ether, ethyl acetate and butyl acetate and the degree of refining was studied by evaluating the properties of the refined residue.

Results obtained show that the removal of the heavy metals decreases with increase in the carbon number of the hydrocarbon solvents, and also decreases with the increase in the molecular weight of the acetate solvents. The percentage removal of vanadium was higher than that of nickel irrespective of the solvents used and ethyl - acetate gave the highest removal of the metals.     

Use of starch and potato peel waste for perchlorate bioreduction in water

The cost of carbon substrates for microbial reduction of perchlorate (ClO(4)(-)) is central to the success and competitiveness of a sustainable bioremediation strategy for ClO(4)(-). This study explored the potential application of starch in combination with an amylolytic bacterial consortia and potato peel waste for ClO(4)(-) bioreduction. We obtained a potent amylolytic bacterial consortium that consisted of a Citrobacter sp. S4, Streptomyces sp. S2, Flavobacterium sp. S6, Pseudoxanthomonas sp. S5, Streptomyces sp. S7, and an Aeromonas sp. S8 identified by 16S rDNA sequencing. ClO(4)(-) concentration substantially decreased in purified starch medium inoculated with the amylolytic bacterial consortium and Dechlorosoma sp. perclace. Potato peel waste supported ClO(4)(-) reduction by perclace with the rate of ClO(4)(-) reduction being dependent on the amount of potato peels. Over 90% ClO(4)(-) removal was achieved in 4 days in a single time point experiment with 2% (w/v) potato peels waste. ClO(4)(-) reduction in a non-sterile 0.5% potato peel media inoculated with perclace occurred with an initial concentration of 10.14+/-0.04 mg L(-1) to 2.87+/-0.4 mg L(-1) (71.7% reduction) within 5 days. ClO(4)(-) was not detected in the cultures in 6 days. In a non-sterile 0.5% potato media without perclace, ClO(4)(-) depletion occurred slowly from an initial value of 9.99+/-0.15 mg L(-1) to 6.33+/-0.43 mg L(-1) (36.63% reduction) in 5 days. Thereafter, ClO(4)(-) was rapidly degraded achieving 77.1% reduction in 7 days and not detected in 9 days. No susbstantial reduction of ClO(4)(-) was observed in the sterile potato peel media without perclace in 7 days. Redox potential of the potato peel cultures was favorable for ClO(4)(-) reduction, decreasing to as low as -294 mV in 24 h. Sugar levels remained very low in cultures effectively reducing ClO(4)(-) and was substantially higher in sterilized controls. Our results indicate that potato peel waste in combination with amylolytic microorganisms and Dechlorosoma sp. perclace can be economically used to achieve complete ClO(4)(-) removal from water.     

Measurement of insolation using CdS photoresistor

Utilizing a cadmium sulphide (CdS) photoresistor, the intensity of solar electromagnetic radiation (insolation) was investigated. Readings were taken daily and hourly at the Nsukka Campus of the University of Nigeria which lies on latitude 6.8° north and longitude 7.35° east. Our investigation spanned a period of 8 months (February–September). This was an extension of work done by E. Klugmann & O. E. Onyeogu who carried out a similar investigation, but for a shorter period, 4 months (October-January) and also their investigations took account of only the dry season and harmattan.

Our investigation took account of a very vital climatic period in Nigeria, namely the rainy season (March–September).

It was observed that with a cadmium sulphide photoresistor which has an average efficiency of 7%², the maximum average hourly insolation is roughly 63 W m⁻² which is equivalent to 900 Wm⁻² if cadmium sulphide were to be 100% efficient. Furthermore, it was consistently observed that the amount of solar intensity falling on our detector is always greater after the rains. Finally, our results are then compared with the modified empirical equations of Rapp & Hoffman and there is some times nice agreement between experimental and theoretical results of hourly insolation in Wm⁻², and sometimes there is sharp contrast between the two.