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1.
Pawlak M. Stadtmuller U. 《IEEE transactions on information theory / Professional Technical Group on Information Theory》1996,42(5):1425-1438
We consider the problem of recovering a band-limited signal f(t) from noisy data yk=f(kτ)+≫epsilon/k, where τ is the sampling rate. Starting from the truncated Whittaker-Shannon cardinal expansion with or without sampling windows (both cases yield inconsistent estimates of f(t)) we propose estimators that are convergent to f(t) in the pointwise and uniform sense. The basic idea is to cut down high frequencies in the data and to use suitable oversampling τ⩽π/Ω, Ω being the bandwidth (maximum frequency) of f(t). The simplest estimator we propose is given by fˆn(t)=τ Σ/|t-kτ|⩽nτ yksin(Ω(t-kτ))/π(t-kτ),|t|⩽nτ. Generalizations of fˆn including sampling windows are also examined. The main aim is to examine the mean squared error (MSE) properties of such estimators in order to determine the optimal choice of the sampling rate τ yielding the fastest possible rate of convergence. The best rate for the MSE we obtain is O(In(n)/n) 相似文献
2.
Lugosi G. Pawlak M. 《IEEE transactions on information theory / Professional Technical Group on Information Theory》1994,40(2):475-481
The posterior-probability estimate of the classification error rate of some nonparametric classification rules is studied. The variance of the estimator is shown to have same remarkable distribution-free properties for the k-nearest neighbor, kernel, and histogram rules. We also investigate the bias of the estimate and establish its consistency and upper bounds. The version of the estimate calculated from an independent set of unclassified patterns is also considered 相似文献
3.
Joanna Czajkowska Adam Junka Jakub Hoppe Monika Toporkiewicz Andrzej Pawlak Pawe Migda Monika Oleksy-Wawrzyniak Karol Fijakowski Marcin
miglak Agata Markowska-Szczupak 《International journal of molecular sciences》2021,22(11)
Staphylococcus aureus is one of the most prevalent pathogens associated with several types of biofilm-based infections, including infections of chronic wounds. Mature staphylococcal biofilm is extremely hard to eradicate from a wound and displays a high tendency to induce recurring infections. Therefore, in the present study, we aimed to investigate in vitro the interaction between S. aureus biofilm and fibroblast cells searching for metabolites that could be considered as potential biomarkers of critical colonization and infection. Utilizing advanced microscopy and microbiological methods to examine biofilm formation and the staphylococcal infection process, we were able to distinguish 4 phases of biofilm development. The analysis of staphylococcal biofilm influence on the viability of fibroblasts allowed us to pinpoint the moment of critical colonization—12 h post contamination. Based on the obtained model we performed a metabolomics analysis by 1H NMR spectroscopy to provide new insights into the pathophysiology of infection. We identified a set of metabolites related to the switch to anaerobic metabolism that was characteristic for staphylococcal biofilm co-cultured with fibroblast cells. The data presented in this study may be thus considered a noteworthy but preliminary step in the direction of developing a new, NMR-based tool for rapid diagnosing of infection in a chronic wound. 相似文献
4.
C. Flötgen M. Pawlak E. Pabo H. J. van de Wiel G. R. Hayes V. Dragoi 《Microsystem Technologies》2014,20(4-5):653-662
Wafer-level Cu–Sn intermetallic bonding is an interesting process for advanced applications in the area of MEMS and 3D interconnects. The existence of two intermetallic phases for Cu–Sn system makes the wafer bonding process challenging. The impact of process parameters on final bonding layer quality have been investigated for transient liquid phase wafer-level bonding based on the Cu–Sn system. Subjects of this investigation were bonding temperature profile, bonding time and contact pressure as well as the choice of metal deposition method and the ratio of deposited metal layer thicknesses. Typical failure modes in intermetallic compound growth for the mentioned process and design parameters have been identified and were subjected to qualitative and quantitative analysis. The possibilities to avoid abovementioned failures are indicated based on experimental results. 相似文献
5.
Effect of Mode of Drying on Microstructure of Potato 总被引:2,自引:0,他引:2
Potato v. Irga was subjected to blanching, and thereafter was dried by convection, puff-drying, and freeze-drying. Microstructure of raw, blanched, and dried tissue was analyzed under the light microscope using computer image analysis. It was found that the tissue of the investigated variety is built up from cells much smaller than those described in literature for other cultivars. Blanching caused starch gelatinization and increase of cross-sectional area of cells. There was no evidence of broken cell walls. Convective drying resulted in cell shrinkage and some breaking of cell walls. It was estimated that some 12% of cells lost integrity during drying. Limited disintegration of the tissue caused by convective drying is attributed to small size of cells, large contact area, and high cohesive forces between cells. Puff-drying damaged the tissue much more than the convective drying. The most devastating to tissue microstructure was freeze-drying, probably freezing per se. 相似文献
6.
Robin R. Murphy Eric Steimle Michael Hall Michael Lindemuth David Trejo Stefan Hurlebaus Zenon Medina-Cetina Daryl Slocum 《Journal of Intelligent and Robotic Systems》2011,64(1):77-95
The Center for Robot-Assisted Search and Rescue (CRASAR®) deployed a customized AEOS man-portable unmanned surface vehicle and two commercially available underwater vehicles (the autonomous YSI EcoMapper and the tethered VideoRay) for inspection of the Rollover Pass bridge in the Bolivar peninsula of Texas in the aftermath of Hurricane Ike. A preliminary domain analysis with the vehicles identified key tasks in subsurface bridge inspection (mapping of the debris field and inspecting the bridge footings for scour), control challenges (navigation under loss of GPS, underwater obstacle avoidance, and stable positioning in high currents without GPS), possible improvements to human-robot interaction (having additional display units so that mission specialists can view and operate on imagery independently of the operator control unit, incorporating 2-way audio to allow operator and field personnel to communicate while launching or recovering the vehicle, and increased state sensing for reliability), and discussed the cooperative use of surface, underwater, and aerial vehicles. The article posits seven milestones in the development of a fully functional UMV for bridge inspection: standardize mission payloads, add health monitoring, improve teleoperation through better human-robot interaction, add 3D obstacle avoidance, improve station-keeping, handle large data sets, and support cooperative sensing. 相似文献
7.
In laser technological processes, which are connected with phase transitions, special changes in material structure occur. These changes of structure include among others phase composition as well as morphology. The changed structure determines the electrical, mechanical and functional properties of the material. The problems become of greater importance in laser microtechnologies when areas with changed structure have dimensions measurable in micrometers, and volume of an order of 10−3 mm3. The favorable and unfavorable effects of the structural changes of materials and functional properties of elements produced by means of laser microtechnology have been analyzed in the paper. 相似文献
8.
9.
Monika Warat Ewelina Szliszka Ilona Korzonek-Szlacheta Wojciech Król Zenon P. Czuba 《International journal of molecular sciences》2014,15(7):11510-11522
Expression level of Tumor Necrosis Factor—related apoptosis—inducing ligand (TRAIL) receptors is one of the most important factors of TRAIL-mediated apoptosis in cancer cells. We here report for the first time data concerning TRAIL-R1 and TRAIL-R2 receptor expression on RAW264.7 macrophages. Three substances belonging to flavones: chrysin, apigenin and acacetin which differ from their substituents at the 4'' position in the phenyl ring were used in assays because of the variety of biological activities (e.g., anticancer activity) of the polyphenol compounds. The expression of TRAIL-R1 and TRAIL-R2 death receptors on non-stimulated and LPS (lipopolysaccharide)-stimulated macrophages was determined using flow cytometry. We demonstrate that RAW264.7 macrophages exhibit TRAIL-R1 surface expression and that the tested compounds: chrysin, apigenin and acacetin can inhibit TRAIL-R1 death receptor expression level on macrophages. 相似文献
10.
James A. Birrell Kathrin Wrede Krzysztof Pawlak Patricia Rodriguez-Maciá Olaf Rüdiger Edward J. Reijerse Wolfgang Lubitz 《Israel journal of chemistry》2016,56(9-10):852-863
Hydrogenases catalyze the reduction of protons and oxidation of molecular hydrogen with high turnover frequencies and low overpotentials under ambient conditions. The heterodimeric [FeFe] hydrogenase from Desulfovibrio desulfuricans has an exceptionally high activity, and can be purified aerobically in an oxygen-stable inactive state. Recently, it was demonstrated that monomeric [FeFe] hydrogenases produced recombinantly in Escherichia coli can be artificially maturated by simply incubating the inactive “apo” enzymes with the synthetic [2Fe] cofactor mimic [Fe2(adt)(CO)4(CN)2]2−. Here, we use the same technique to produce the heterodimeric “apo” hydrogenase from D. desulfuricans in E. coli with a high yield and purity, and maturate the “apo” enzyme with [Fe2(adt)(CO)4(CN)2]2− to generate fully active “holo” enzyme. Interestingly, the rate of the artificial maturation process with D. desulfuricans is significantly slower than that for all other hydrogenases tested so far. The artificially maturated enzyme is spectroscopically and electrochemically identical to the native enzyme and shows high rates of hydrogen production (3700 s−1) and hydrogen oxidation (63,000 s−1). We expect that our highly efficient production method will facilitate future studies of this enzyme and other related [FeFe] hydrogenases from Desulfovibrio species. 相似文献