Detection of anti-HIV-1 IgG antibodies in whole saliva by GACELISA and Western blot assays

The present study, based on 158 HIV seropositives and 167 HIV seronegatives, demonstrates that saliva collected with the Omni-SAL device and tested with GACELISA (an IgG antibody capture ELISA) is an effective non-invasive alternative to serum for anti-HIV IgG antibody screening. The study also shows that a conventional serum Western blot kit can be used, with slight modifications, for confirmatory testing of saliva specimens. Collecting saliva with the Omni-SAL device had a very good acceptance rate among Tanzanian subjects, and although this diagnostic method is not yet known by the general public, 65% of the study participants preferred to give saliva instead of blood for HIV testing.     

Modulation resonance enhancement in SCH quantum-well lasers with anexternal Bragg reflector

The modulation response of a semiconductor laser can be enhanced by coupling it to an external cavity with frequency-selective feedback. This creates a comb of transmission bands where the modulation response is high, at the cavity round-trip frequency and its harmonics. In a previous publication, we related the bandwidths of these bands to the material and structural parameters of a bulk laser. We showed that a nonzero linewidth enhancement factor together with a nonzero intermediate facet reflectivity lead to deep nulls close to the peaks of these transmission bands. This suggests that quantum-well (QW) lasers, which have a low linewidth enhancement factor, may give a better performance than bulk lasers. To test this hypothesis, we have extended our analysis to model QW lasers coupled to a fiber grating. Carrier transport, carrier heating, intraband carrier fluctuations, and nonparabolic band structures are considered. We show that electron carrier transport and amplitude-phase coupling in the separate-confinment-heterostructure (SCH) layer contribute to the nulls in the modulation response. Therefore, the apparent advantage of having a reduced linewidth enhancement factor that we found in our previous analysis cannot be fully realized by using QW lasers     

Predicting Missing Items in Shopping Carts

Existing research in association mining has focused mainly on how to expedite the search for frequently co-occurring groups of items in “shopping cart” type of transactions; less attention has been paid to methods that exploit these “frequent itemsets” for prediction purposes. This paper contributes to the latter task by proposing a technique that uses partial information about the contents of a shopping cart for the prediction of what else the customer is likely to buy. Using the recently proposed data structure of itemset trees (IT-trees), we obtain, in a computationally efficient manner, all rules whose antecedents contain at least one item from the incomplete shopping cart. Then, we combine these rules by uncertainty processing techniques, including the classical Bayesian decision theory and a new algorithm based on the Dempster-Shafer (DS) theory of evidence combination.     

Degradation kinetics of ascorbic acid during ohmic heating with stainless steel electrodes

Ascorbic acid degradation experiments were performed in buffer solution at pH 3.5 using a batch ohmic heater with uncoated stainless steel electrodes. The electrical conductivity of the buffer solution was adjusted using sodium chloride. The concentration of ascorbic acid was found using an HPLC technique. Kinetics of degradation can be described adequately by a first order model for both conventional and ohmic treatments, but unlike conventional heating, the temperature dependence of degradation for some ohmic treatments cannot be represented by the Arrhenius relation. During ohmic heating, power, temperature and NaCl content affect the degradation rate. A number of reactions, including electrode reactions, electrolysis of the solution, as well as reactions between electrode materials and electrolysis products may influence the reaction mechanism as well as kinetic parameters. At the highest power and salt content, citrate complexation and a significant loss of buffering capacity were noted, resulting in an increase in pH. The results underline the importance of inert electrode coatings, or the use of high frequency power for control of electrochemical reactions.     

The effect of limited exposure to antimycotics on the relative cell-surface hydrophobicity and the adhesion of oral Candida albicans to buccal epithelial cells

Candida albicans is the major aetiological agent of oral candidosis. Adhesion to oral mucosal surfaces is considered a prerequisite for its successful colonization and subsequent infection, and its relative cell-surface hydrophobicity (CSH) is a contributory physical force. Thus, the main aim here was to determine the CSH of 10 isolates of oral C. albicans after a short exposure to sublethal concentrations of four antifungal agents and to correlate these findings with their adhesion to human buccal epithelial cells (BEC). The yeasts were exposed to sublethal concentrations of nystatin [x 6 minimal inhibitory concentration (MIC)], 5-fluorocytosine (x 8 MIC), ketoconazole (x 4 MIC) and fluconazole (x 4 MIC) for 1 h. The drug was then removed, and the CSH and BEC adhesion assessed by a biphasic aqueous-hydrocarbon assay and a microscopic method, respectively. The mean percentage reductions of CSH after exposure to nystatin, 5-fluorocytosine, ketoconazole and fluconazole were 27.14% (p = 0.01), 9.46% (p = 0.43), 19.47% (p = 0.04) and 6.16% (p = 0.59). Similarly, exposure to all the drugs except 5-fluorocytosine resulted in a significant inhibition of yeast adhesion to BEC, with nystatin eliciting the highest and fluconazole the least inhibition. Further, on regression analysis a strong positive correlation was observed between CSH and adhesion to BEC after limited exposure to 5-fluorocytosine (r = 0.48, p < 0.0001), ketoconazole (r = 0.48, p < 0.0001), fluconazole (r = 0.55, p < 0.0001) as well as in the unexposed controls (r = 0.41, p = 0.001), although nystatin was an exception (r = 0.09, p = 0.44). Taken together, these data elucidate further mechanisms by which antimycotics may operate in vivo to suppress candidal pathogenicity.     

Clinical predictors of HIV-1 infection among preschool children in Dar es Salaam, Tanzania

Seroprevalence of HIV-1 infection was determined in children aged between eighteen months and five years, attending maternal and child health (MCH) clinics in Dar es Salaam, Tanzania. A total of 889 children were eligible for the study, however seven children could not be enrolled because their mothers/guardians absconded and blood could not be drawn from 21 children due to refusal of mothers/guardians and from another 12 children due to technical reasons. Therefore, the participation rate was 95.5%. Of the 849 children screened, 14 (1.65%) were found to have IgG anti HIV-1 antibodies in their sera. The main clinical features found in children with symptomatic HIV-1 disease were weight loss, generalized lymphadenopathy, recurrent fevers, and prolonged diarrhoea. The utility of clinical features suggestive of HIV-1 infection (according to CDC classification) in identifying HIV-1 infection in children was evaluated and found to have high sensitivity (100%), specificity (96.9%) and negative predictive value (100%), but a low positive predictive value (35%). Marked variations in progression to symptomatic phase were noted, whereby some relatively young children had progressed to symptomatic phase (CDC class P-2A), while some older children were still in the asymptomatic stage (CDC class P-1 C). None of the symptomatic HIV-1 infected children presented with neurological disease, severe opportunistic infections, or malignancies. Although reduced mid-upper arm circumference and weight-for-age were associated with HIV seropositivity, these clinical parameters had low positive predictive values compared to the CDC classification.     

Oral endotoxin in healthy adults

A case is presented which is thought to be the first described example of rib fracture occurring as a result of airbag inflation. It would appear that the propellant cartridge came loose during deployment to form a missile, striking the patient on his chest and fracturing a rib.     

THE ASYMPTOTIC PROPERTIES OF THE SAMPLE AUTOCORRELATIONS FOR A MULTIPLE AUTOREGRESSIVE PROCESS WITH ONE UNIT ROOT

Abstract. In this paper the large sample behaviour of the sample autocorrelation matrix R _n( h ), ( h being the lag, n the sample size), of a multivariate autoregressive time series with one of its characteristic roots equal to unity and the rest of the roots lying inside the unit circle is studied. It is shown that R _n( h ) converges almost surely to a constant matrix. Further, the asymptotic distribution of R _n( h ) is characterized as that of a random matrix which is a function of jointly normal random variables.