A Long Noncoding RNA ZEB1-AS1 Promotes Tumorigenesis and Predicts Poor Prognosis in Glioma

Emerging studies show that long noncoding RNAs (lncRNAs) have important roles in carcinogenesis. lncRNA ZEB1 antisense 1 (ZEB1-AS1) is a novel lncRNA, whose clinical significance, biological function, and underlying mechanism remains unclear in glioma. Here, we found that ZEB1-AS1 was highly expressed in glioma tissues, being closely related to clinical stage of glioma. Moreover, patients with high ZEB1-AS1 levels had poor prognoses, with the evidence provided by multivariate Cox regression analysis indicating that ZEB1-AS1 expression could serve as an independent prognostic factor in glioma patients. Functionally, silencing of ZEB1-AS1 could significantly inhibit cell proliferation, migration, and invasion, as well as promote apoptosis. Knockdown of ZEB1-AS1 significantly induced the G0/G1 phase arrest and correspondingly decreased the percentage of S phase cells. Further analysis indicated that ZEB1-AS1 could regulate the cell cycle by inhibiting the expression of G1/S transition key regulators, such as Cyclin D1 and CDK2. Furthermore, ZEB1-AS1 functioned as an important regulator of migration and invasion via activating epithelial to mesenchymal transition (EMT) through up-regulating the expression of ZEB1, MMP2, MMP9, N-cadherin, and Integrin-β1 as well as decreasing E-cadherin levels in the metastatic progression of glioma. Additionally, forced down-regulation of ZEB1-AS1 could dramatically promote apoptosis by increasing the expression level of Bax and reducing Bcl-2 expression in glioma. Taken together, our data suggest that ZEB1-AS1 may serve as a new prognostic biomarker and therapeutic target of glioma.     

云南木姜子提取物对蜡样芽孢杆菌抑菌作用的研究

目的 研究云南木姜子(Yunnan Litsea cubeba)的抑菌活性及抑菌稳定性。方法 用乙醇对云南木姜子果实进行提取并分别用石油醚、氯仿、乙酸乙酯、正丁醇进行萃取。比较不同萃取相对蜡样芽孢杆菌的抑制作用,并通过热处理、酸碱处理、紫外照射、金属离子处理、糖和盐处理对其稳定性进行考察。结果 云南木姜子提取物对蜡样芽孢杆菌具有良好的抑菌作用,其活性成分主要分布于乙酸乙酯相,其对蜡样芽孢杆菌的最小抑菌浓度为0.08 mg/mL;云南木姜子乙酸乙酯萃取相经紫外线照射、酸碱处理和不同温度处理仍表现出良好的稳定性,盐和糖处理条件使其对蜡样芽孢杆菌的抑菌活性增强,对于金属离子处理,Mg²⁺使其对蜡样芽孢杆菌的抑菌活性提高,Fe³⁺、Fe²⁺、Al³⁺和Ca²⁺等使其抑菌活性降低。结论 云南木姜子提取物具有良好的抑菌活性,温度、紫外和pH对其抑菌成分的稳定性有较小的影响,金属离子、糖和盐浓度对其抑菌活性影响较大。因此可考虑将云南木姜子作为一种天然植物源防腐剂,它在保鲜方面具有良好的开发和...     

HACCP体系在黑木耳主产区质控中的应用

目的：为响应乡村振兴战略,建立产业发展中的质控体系,确保黑木耳初级产品的质量安全管理问题。方法：应用HACCP体系的基本原理,对黑木耳生产过程进行危害分析,通过关键控制点判断树确定关键控制点,并确立关键限值及纠偏措施。结果：将HACCP体系应用于黑木耳主产区生产过程中,确定该过程的原材料的选择、菌种的选择、营养生长阶段、生殖生长阶段、黑木耳采收加工和菌糠的处理6个关键控制点。结论：预防或降低黑木耳生产过程中的危害,确保黑木耳质量安全,进而促进黑木耳产业可持续的发展。     

Silencing the SpMPK1, SpMPK2, and SpMPK3 Genes in Tomato Reduces Abscisic Acid—Mediated Drought Tolerance

Drought is a major threat to agriculture production worldwide. Mitogen-activated protein kinases (MAPKs) play a pivotal role in sensing and converting stress signals into appropriate responses so that plants can adapt and survive. To examine the function of MAPKs in the drought tolerance of tomato plants, we silenced the SpMPK1, SpMPK2, and SpMPK3 genes in wild-type plants using the virus-induced gene silencing (VIGS) method. The results indicate that silencing the individual genes or co-silencing SpMPK1, SpMPK2, and SpMPK3 reduced the drought tolerance of tomato plants by varying degrees. Co-silencing SpMPK1 and SpMPK2 impaired abscisic acid (ABA)-induced and hydrogen peroxide (H₂O₂)-induced stomatal closure and enhanced ABA-induced H₂O₂ production. Similar results were observed when silencing SpMPK3 alone, but not when SpMPK1 and SpMPK2 were individually silenced. These data suggest that the functions of SpMPK1 and SpMPK2 are redundant, and they overlap with that of SpMPK3 in drought stress signaling pathways. In addition, we found that SpMPK3 may regulate H₂O₂ levels by mediating the expression of CAT1. Hence, SpMPK1, SpMPK2, and SpMPK3 may play crucial roles in enhancing tomato plants’ drought tolerance by influencing stomatal activity and H₂O₂ production via the ABA-H₂O₂ pathway.     

米索前列醇在宫腔镜检查中的作用及其评价

目的:观察米索前列醇在宫腔镜检查时软化宫颈及镇痛的效果。方法:将宫腔镜检查的107 例病人随机分为2 组:米索前列醇组63 例, 检查前1 h口服米索前列醇200 μg, 对照组44 例不施任何药物。结果:宫腔镜检查米索前列醇组宫口扩张直径、术中出血、人工流产综合症(PAAS) 发生率及腹痛程度均明显低于对照组(P <0.05) 。结论:宫腔镜检查前1 h 口服米索前列醇200 μg 为方便、有效软化宫颈的理想方法, 可缓解有创检查性疼痛, 减少手术操作, 减少术中出血, 降低PAAS 的发生, 值得临床推广应用。     

New strong convergence method for the sum of two maximal monotone operators

Optimization and Engineering - This paper aims to obtain a strong convergence result for a Douglas–Rachford splitting method with inertial extrapolation step for finding a zero of the sum of...     

基于移动互联网的食品生产许可现场核查系统的应用效果

目的分析评价基于移动互联网的核查系统移动应用软件(APP)在食品生产许可现场核查工作中的应用效果。方法选择48家企业开展核查系统测试,将审查员随机分为实验组和对照组,实验组采用有核查系统APP进行核查工作,对照组则采用传统纸质评分表格进行核查工作。比较2组的工作用时、资料完整性和差错概率方面的差异。结果与对照组比较,实验组在现场核查工作用时、资料完整性和差错性的差异均有统计学意义。实验组和对照组在实用性和公平性的评价差异有统计学意义,在规范性的评价差异无统计学意义。结论食品生产许可现场核查系统APP实用性强,在保证现场核查工作程序规范性的前提下,提高了工作效率、质量和公平性,值得推广应用。     

基于基址重定位的快速域名压缩算法

为了提高DNS服务器的性能,提出了基于M/M/c排队系统的分析模型.利用该模型分析了DNS服务器的响应时间百分比,确定了其性能瓶颈在于域名压缩速率.目前传统压缩算法由于规则的限制只能在查询应答时进行实时的域名压缩,在高访问量场景存在性能问题.为了提高压缩速率,从DNS数据特征方面对域名压缩的原理进行了剖析,并在此基础上结合重定位技术,提出一种新的域名压缩算法.新的设计改变了传统的DNS数据处理流程,通过压缩前置,降低了应答的实时消耗.实验结果表明,该算法在压缩比损失很小的条件下提升了系统资源利用率,达到了优化响应时间百分比的目的.     

有门限的动态双队列缓存管理策略性能分析

对共享缓存分组交换机提出了按需分配缓存的具有门限的动态双队列缓存管理策略.具有门限的动态双队列特性,在提高不同优先级业务服务公平性以提供服务质量保证的同时,也提高了缓存的整体有效利用率.据此对该系统建立了M1 M2/M/1/K (K)的排队模型,给出了相应的Q矩阵,利用矩阵几何解方法对模型求解得到了稳态概率分布.最后做了相应的性能分析,定量给出了不同优先级分组的队长分布、丢失概率等的计算公式.