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We have developed an easy-to-use computer-based system for recording, displaying, storing and analyzing signals generated by Clark-type oxygen electrodes. A user-friendly interface of Windows-based program BioMed significantly increases the productivity of investigations. It allows to process, control, present and archive the experimental data in real time. A 12-bit analog-to-digital-converter, analog and digital filters, a possibility to zoom the obtained respiratory curves and calculation of the respiration rates by a linear regression method increase the resolution of the estimated oxygen consumption rates. The new system enables to register even small changes, such as 3-5 ngatoms O/min, in respiration rates of biological objects -- enzymes, mitochondria and permeabilized muscle fibers. The system has been developed and is regularly used for the respirometric investigations at the Laboratory of Biochemistry, Institute for Biomedical Research, Kaunas University of Medicine.  相似文献   
2.
In this work we present an optical refractive index (RI) sensor based on the spectral analysis of anomalies in the optical response from the diffraction grating employing polarized polychromatic light. The sensor consists of holographic diffraction grating (period 423.5 nm) coated with a thin (110 nm) SiOx doped diamond like carbon film (DLC) that defines sensitivity of the sensor as well as the range of the spectral analysis. The deposition of the DLC film (synthesized by the direct ion beam deposition from the hydrocarbon source) has influence on the shape but not on the position of the anomalies observed in the specular reflection spectrum. From the reflection spectra the RI dispersion curve of liquid analyte-water was obtained.  相似文献   
3.
An analytical model is presented to predict the nonlinear response of a double-wall sandwich cylindrical shell system subjected to random excitation. Nonlinear spring-dashpot models are integrated into the system to characterize the behavior of the soft core. Donnell’s thin shell theory is used to develop the governing nonlinear equations of motion. A Monte Carlo simulation of stationary random processes, multimode Galerkin-type approach, and numerical integration procedures are used to develop linear and nonlinear response solutions of simply supported cylindrical shells. Numerical results include time domain response histories, root-mean-square values and response spectral densities. Parametric studies are performed to investigate the effects of nonlinearity, shell thickness, core stiffness, and thickness.  相似文献   
4.
The surface protection systems of aerospace and aircraft structures are often constructed from discretely stiffened panels. The information that is available indicates that response of surface panels exposed to high intensity surface flow and/or acoustic pressure is nonlinear. A theoretical study on nonlinear response of discretely stiffened panels to random pressures and thermal loads is presented in this paper. The random pressures are simulated in time-space domain and the nonlinear equations of motion are solved numerically by a Monte Carlo type approach. In addition, preliminary estimates of sonic fatigue based on the nonlinear stress distribution of response peaks and fatigue data from constant amplitude tests are given. The numerical results include deflection and stress response time histories, spectral densities, root mean square values, crossing rates, probability distribution, peak distribution and fatigue damage.  相似文献   
5.
The structure-borne noise generation and transmission of stiffened and interconnected structures under random loads is presented. The method is based on the transfer matrix for the structural response and on the modal decomposition for the interior acoustic field. The acoustic enclosure is taken to be rectangular in shape of which portion of the boundaries are elastic while the remaining surface is acoustically rigid. Numerical results are presented for a variety of acousto-structural problems.  相似文献   
6.
We describe an active optical system that both measures and corrects the aberrations introduced when writing three-dimensional bit-oriented optical memory by a two-photon absorption process. The system uses a ferroelectric liquid-crystal spatial light modulator (FLCSLM) configured as an arbitrary wave-front generator that is reconfigurable at speeds as great as 2.5 kHz. A method of aberration measurement by the FLCSLM wave-front generator is described. The same device is also used to correct the induced aberrations by preshaping the wave fronts with the conjugate phase aberration as well as to scan the focal spot in three dimensions. Experimental results show the correction of both on- and off-axis aberrations, allowing the writing of data at depths as great as 1 mm inside a LiNbO3 crystal.  相似文献   
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