Cyclic Nigerosyl-Nigerose as Oxygen Nanocarrier to Protect Cellular Models from Hypoxia/Reoxygenation Injury: Implications from an In Vitro Model

Heart failure (HF) prevalence is increasing among the aging population, and the mortality rate remains unacceptably high despite improvements in therapy. Myocardial ischemia (MI) and, consequently, ischemia/reperfusion injury (IRI), are frequently the basis of HF development. Therefore, cardioprotective strategies to limit IRI are mandatory. Nanocarriers have been proposed as alternative therapy for cardiovascular disease. Controlled reoxygenation may be a promising strategy. Novel nanocarriers, such as cyclic nigerosyl-nigerose (CNN), can be innovative tools for oxygen delivery in a controlled manner. In this study we analyzed new CNN-based formulations as oxygen nanocarriers (O₂-CNN), and compared them with nitrogen CNN (N₂-CNN). These different CNN-based formulations were tested using two cellular models, namely, cardiomyoblasts (H9c2), and endothelial (HMEC) cell lines, at different concentrations. The effects on the growth curve during normoxia (21% O₂, 5% CO₂ and 74% N₂) and their protective effects during hypoxia (1% O₂, 5% CO₂ and 94% N₂) and reoxygenation (21% O₂, 5% CO₂ and 74% N₂) were studied. Neither O₂-CNN nor N₂-CNN has any effect on the growth curve during normoxia. However, O₂-CNN applied before hypoxia induces a 15–30% reduction in cell mortality after hypoxia/re-oxygenation when compared to N₂-CNN. O₂-CNN showed a marked efficacy in controlled oxygenation, which suggests an interesting potential for the future medical application of soluble nanocarrier systems for MI treatment.     

Influence of Gluconate, Lignosulfonate, and Glucose Admixtures on the Hydration of Tetracalcium Aluminoferrite in the Presence of Gypsum with or without Calcium Hydroxide

The influence of 0.3 wt% gluconute, lignosulfonate, or glucose on the hydration of 4CaO·A1₂O₃-Fe₂O₃ in the presence of gypsum with or without Ca(OH)₂, was examined. In the absence of Ca(OH)₂ all the admixtures retard both ettringite production and subsequent conversion of ettringite into the monosulfate in the decreasing order glucose >lignosulfonate >gluconate. In the presence ofCa(OH)₂ all the admixtures accelerate early ettringite production but do not affect subsequent conversion of ettringite into the monosulfate, at least up to 28 d.     

University-owned and university-invented patents: a network analysis on two Italian universities

The paper presents results from social network analysis applied to data on patenting of academics inventors employed in two Italian universities (Trieste University and Udine university, both located in Friuli Venezia Giulia region). The aim is to compare the co-invention networks generated by the academic inventors, tenured by one of the two universities, in their patenting activity with several organisations—firms, public research organisations—and in their activity for patents owned by one of the two universities. Results show that, despite the structural similarity, non-marginal differences emerge in the interaction of the two forms of patenting across the two universities. Empirical evidence suggests new research questions related in particular to the role played by the differing university patenting strategies in shaping local networks.     

Combined effect of lignosulfonate and carbonate on pure portland clinker compounds hydration. III. Hydration of tricalcium silicate alone and in the presence of tricalcium aluminate

The effect of carbonate and/or lignosulfonate on the hydration of C₃S alone and in the presence of C₃A has been examined by DTG and TG curves and by zeta potential measurements. The combined addition of sodium carbonate and lignosulfonate strongly retards C₃A hydration. However by mixing 20 % C₃A with C₃S the retarding effect is significantly lower. On the other hand the early C₃A hydration is completely blocked by sodium carbonate and lignosulfonate simultaneously added. It seems that the fluidifying effect of the combined addition of those admixtures could be ascribed to both the dispersing action and the completely blocking effect on the early C₃A hydration.     

Percutaneous Coronary Intervention (PCI) Reprograms Circulating Extracellular Vesicles from ACS Patients Impairing Their Cardio-Protective Properties

Extracellular vesicles (EVs) are promising therapeutic tools in the treatment of cardiovascular disorders. We have recently shown that EVs from patients with Acute Coronary Syndrome (ACS) undergoing sham pre-conditioning, before percutaneous coronary intervention (PCI) were cardio-protective, while EVs from patients experiencing remote ischemic pre-conditioning (RIPC) failed to induce protection against ischemia/reperfusion Injury (IRI). No data on EVs from ACS patients recovered after PCI are currently available. Therefore, we herein investigated the cardio-protective properties of EVs, collected after PCI from the same patients. EVs recovered from 30 patients randomly assigned (1:1) to RIPC (EV-RIPC) or sham procedures (EV-naive) ({"type":"clinical-trial","attrs":{"text":"NCT02195726","term_id":"NCT02195726"}}NCT02195726) were characterized by TEM, FACS and Western blot analysis and evaluated for their mRNA content. The impact of EVs on hypoxia/reoxygenation damage and IRI, as well as the cardio-protective signaling pathways, were investigated in vitro (HMEC-1 + H9c2 co-culture) and ex vivo (isolated rat heart). Both EV-naive and EV-RIPC failed to drive cardio-protection both in vitro and ex vivo. Consistently, EV treatment failed to activate the canonical cardio-protective pathways. Specifically, PCI reduced the EV-naive Dusp6 mRNA content, found to be crucial for their cardio-protective action, and upregulated some stress- and cell-cycle-related genes in EV-RIPC. We provide the first evidence that in ACS patients, PCI reprograms the EV cargo, impairing EV-naive cardio-protective properties without improving EV-RIPC functional capability.     

Scleroderma-like Impairment in the Network of Telocytes/CD34+ Stromal Cells in the Experimental Mouse Model of Bleomycin-Induced Dermal Fibrosis

Considerable evidence accumulated over the past decade supports that telocytes (TCs)/CD34⁺ stromal cells represent an exclusive type of interstitial cells identifiable by transmission electron microscopy (TEM) or immunohistochemistry in various organs of the human body, including the skin. By means of their characteristic cellular extensions (telopodes), dermal TCs are arranged in networks intermingled with a multitude of neighboring cells and, hence, they are thought to contribute to skin homeostasis through both intercellular contacts and releasing extracellular vesicles. In this context, fibrotic skin lesions from patients with systemic sclerosis (SSc, scleroderma) appear to be characterized by a disruption of the dermal network of TCs, which has been ascribed to either cell degenerative processes or possible transformation into profibrotic myofibroblasts. In the present study, we utilized the well-established mouse model of bleomycin-induced scleroderma to gain further insights into the TC alterations found in cutaneous fibrosis. CD34 immunofluorescence revealed a severe impairment in the dermal network of TCs/CD34⁺ stromal cells in bleomycin-treated mice. CD31/CD34 double immunofluorescence confirmed that CD31⁻/CD34⁺ TC counts were greatly reduced in the skin of bleomycin-treated mice compared with control mice. Ultrastructural signs of TC injury were detected in the skin of bleomycin-treated mice by TEM. The analyses of skin samples from mice treated with bleomycin for different times by either TEM or double immunostaining and immunoblotting for the CD34/α-SMA antigens collectively suggested that, although a few TCs may transition to α-SMA⁺ myofibroblasts in the early disease stage, most of these cells rather undergo degeneration, and then are lost. Taken together, our data demonstrate that TC changes in the skin of bleomycin-treated mice mimic very closely those observed in human SSc skin, which makes this experimental model a suitable tool to (i) unravel the pathological mechanisms underlying TC damage and (ii) clarify the possible contribution of the TC loss to the development/progression of dermal fibrosis. In perspective, these findings may have important implications in the field of skin regenerative medicine.     

Mechanisms of Sensitivity and Resistance of Primary Effusion Lymphoma to Dimethyl Fumarate (DMF)

PEL is a rare B cell lymphoma associated with KSHV that mainly arises in immune-deficient individuals. The search for new drugs to treat this cancer is still ongoing given its aggressiveness and the poor response to chemotherapies. In this study, we found that DMF, a drug known for its anti-inflammatory properties which is registered for the treatment of psoriasis and relapsing–remitting MS, could be a promising therapeutic strategy against PEL. Indeed, although some mechanisms of resistance were induced, DMF activated NRF2, reduced ROS and inhibited the phosphorylation of STAT3 and the release of the pro-inflammatory and immune suppressive cytokines IL-6 and IL-10, which are known to sustain PEL survival. Interestingly, we observed that DMF displayed a stronger cytotoxic effect against fresh PEL cells in comparison to PEL cell lines, due to the activation of ERK1/2 and autophagy in the latter cells. This finding further encourages the possibility of using DMF for the treatment of PEL.     

Combined effect of lignosulfonate and carbonate on pure portland clinker compounds hydration. V. Hydration of dicalcium silicate alone and in the presence of tricalcium aluminate

The effect of sodium carbonate and/or sodium lignosulfonate on the hydration of C₂S alone and in the presence of C₃A has been examinated by DTG and TG curves and by zeta potential measurements. The combined addition of sodium carbonate and lignosulfonate retards the C₂S hydration to a lower extent than that observed for the C₃S hydration. The retarding effect on the C₂S hydration is significantly lower in the presence of 20% C₃A. On the other hand, the early C₃A hydration is completely blocked by admixtures simultaneously added. Addition of 0.9% sodium carbonate without lignosulfonate blocks the early hydration of both C₃A and C₂S. This effect was not found in the C₃SC₃A system.