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The Frizzled genes encode receptors for WNTs, secreted glycoproteins implicated in development as well as in carcinogenesis. In this paper, we report molecular cloning of Hfz6, the human homologue of Mfz6. Nucleotide sequence analysis showed that the Hfz6 gene encodes the 706 amino-acid protein with seven transmembrane domains, a cystein-rich domain in the N-terminal extracellular region, two N-linked glycosylation sites, and two cystein residues in the second and third extracellular loops. Hfz6 mRNA 4.4-kb in size was detected in various normal adult and fetal tissues, and a larger amount of Hfz6 mRNA was detected in both fetal lung and fetal kidney. The Hfz6 gene has been mapped to human chromosome 8q22.3-q23.1. In conclusion, we have cloned Hfz6, which encodes a seven-transmembrane receptor with the cystein-rich domain in the N-terminal extracellular region, but without the Ser/Thr-X-Val motif in the C-terminus. 相似文献
3.
S Maeshima T Terada N Yoshida K Nakai T Itakura N Komai 《Canadian Metallurgical Quarterly》1997,78(6):666-669
A mechanical computerized three-dimensional scanner with a resolution of 1 micron was used to assess loss of enamel caused by orthodontic bonding and debonding. A total of 2646 measurements was performed on six human premolars. The results showed an average loss of enamel of 7.4 microns. The range was between 1 and 52 microns, which may account for discrepancies with earlier studies that measured only a few points per tooth surface. 相似文献
4.
Seshita T. Ikeda Y. Wakimoto H. Ishida K. Terada T. Matsunaga T. Suzuki T. Kitaura Y. Uchitomi N. 《Solid-State Circuits, IEEE Journal of》1994,29(12):1583-1588
An ultrahigh-speed 8 bit multiplexer (MUX) has been developed for future-generation optical-fiber communication systems having a data rate of 20 Gb/s. This IC was fabricated using a 0.5 μm WNx/W-gate GaAs MESFET process based on optical lithography, ion implantation, and furnace annealing for good reproducibility and high throughput. The WNx/W bilayer gate has a low sheet resistance, improving the circuit high frequency performance. To attain 20 GHz operation, advanced circuit techniques for the source-coupled FET logic (SCFL) were introduced. A cross coupled source-follower (CCSF) was developed mainly for the highest speed buffers to enhance the bandwidth. The first-stage T-type flip-flop was designed with optimization techniques and operated up to 21.1 GHz 相似文献
5.
Infectious mononucleosis (IM) syndrome is typically caused by EBV, but also by drugs and other organisms such as CMV and HBV. It demonstrates a wide range of clinical and laboratory characteristics, presumably depending on the age of onset. However, associations of laboratory abnormalities with the clinical features have not been well documented. We evaluated here, the associations among patients with IM syndrome using of multiple regression (MR) and multiple logistic regression (MLR) analyses. We examined 90 (40 males, 50 females) patients, who were admitted to our hospital with IM syndrome. The diagnostic criteria were fever and presence of atypical lymphocytes (> 5% of the WBC or the count including monocytes > 5000/microliter), and at least 3 of 7 clinical features: tonsillitis, lymphadenopathy, skin rash, hepato-, spleno-megaly, hepatic dysfunction. The diagnosis of EBV was serologically confirmed in 41 cases. MR revealed that the higher age group tended to have lower platelet counts, and that lower platelet counts were associated with higher ALT levels. In addition, MLR revealed that patients with skin rash tended not to have splenomegaly. The frequency of splenomegaly was not related to age, contrary to the findings of previous reports. These findings are useful to differentiate IM syndrome based on laboratory data. 相似文献
6.
Masaru Miyayama Hiroya Terada Hiroaki Yanagida 《Journal of the American Ceramic Society》1981,64(1):19-C-
The metastable β form of bismuth sesquioxide was obtained by doping antimony oxide. The solubility of antimony was 4 to 10 at. %in β specimens, where more than 75% of the antimony atoms were valenced at 5+. 相似文献
7.
A high temperature Seebeck coefficient measurement apparatus with various features to minimize typical sources of error is designed and built. Common sources of temperature and voltage measurement error are described and principles to overcome these are proposed. With these guiding principles, a high temperature Seebeck measurement apparatus with a uniaxial 4-point contact geometry is designed to operate from room temperature to over 1200 K. This instrument design is simple to operate, and suitable for bulk samples with a broad range of physical types and shapes. 相似文献
8.
Allinson M Kageyama S Nakajima D Kamata R Shiraishi F Goto S Salzman SA Allinson G 《Water science and technology》2012,66(4):768-774
In 2007, samples of treated effluent were collected at point of discharge to the environment from 39 wastewater treatment plants (WWTPs) located across Victoria, Australia grouped by treatment type. Sample genotoxicity was assessed with a high-throughput luminescent umu test method using Salmonella typhimurium TL210 strain, with and without addition of a commercially available metabolic activation system. Samples were also screened using a gas chromatographic-mass spectrometric mass-structure database recognition method. A genotoxic response was observed in half of the samples tested without metabolic activation system (相似文献
9.
Kenjiro Terada Mao Kurumatani 《International journal for numerical methods in engineering》2005,63(15):2102-2123
In this paper an integrated procedure for three‐dimensional (3D) structural analyses with the finite cover method (FCM) is introduced. In the pre‐process of this procedure, the geometry of a structure is modelled by 3D‐CAD, followed by digitization to have the corresponding voxel model, and then the structure is covered by a union of mathematical covers, namely a mathematical mesh independently generated for approximation purposes. Since the mesh topology in the FCM does not need to conform to the physical boundaries of the structure, the mesh can be regular and structured. Thus, the numerical analysis procedure is free from the difficulties mesh generation typically poses and, in this sense, enables us to realize the mesh‐free analysis. After formulating the FCM with interface elements for the static equilibrium state of a structure, we detail the procedure of the finite cover modelling, including the geometry modelling with 3D‐CAD and the identification of the geometry covered by a regular mesh for numerical integration. Prior to full 3D modelling and analysis, we present a simple numerical example to confirm the equivalence of the performance of the FCM and that of the standard finite element method (FEM). Finally, representative numerical examples are presented to demonstrate the capabilities of the proposed analysis procedure. Copyright © 2005 John Wiley & Sons, Ltd. 相似文献
10.
Ryoka Uchiyama Eriko Toyoda Miki Maehara Shiho Wasai Haruka Omura Masahiko Watanabe Masato Sato 《International journal of molecular sciences》2021,22(5)
Osteoarthritis of the knee (OAK) is a chronic degenerative disease and progresses with an imbalance of cytokines and macrophages in the joint. Studies regarding the use of platelet-rich plasma (PRP) as a point-of-care treatment for OAK have reported on its effect on tissue repair and suppression of inflammation but few have reported on its effect on macrophages and macrophage polarization. Based on our clinical experience with two types of PRP kits Cellaid Serum Collection Set P type kit (leukocyte-poor-PRP) and an Autologous Protein Solution kit (APS leukocyte-rich-PRP), we investigated the concentrations of humoral factors in PRPs prepared from the two kits and the effect of humoral factors on macrophage phenotypes. We found that the concentrations of cell components and humoral factors differed between PRPs purified using the two kits; APS had a higher concentration of M1 and M2 macrophage related factors. The addition of PRP supernatants to the culture media of monocyte-derived macrophages and M1 polarized macrophages revealed that PRPs suppressed M1 macrophage polarization and promoted M2 macrophage polarization. This research is the first to report the effect of PRPs purified using commercial kits on macrophage polarization. 相似文献