Crystal structure of tRNA-guanine transglycosylase: RNA modification by base exchange

tRNA-guanine transglycosylases (TGT) are enzymes involved in the modification of the anticodon of tRNAs specific for Asn, Asp, His and Tyr, leading to the replacement of guanine-34 at the wobble position by the hypermodified base queuine. In prokaryotes TGT catalyzes the exchange of guanine-34 with the queuine (.)precursor 7-aminomethyl-7-deazaguanine (preQ1). The crystal structure of TGT from Zymomonas mobilis was solved by multiple isomorphous replacement and refined to a crystallographic R-factor of 19% at 1.85 angstrom resolution. The structure consists of an irregular (beta/alpha)8-barrel with a tightly attached C-terminal zinc-containing subdomain. The packing of the subdomain against the barrel is mediated by an alpha-helix, located close to the C-terminus, which displaces the eighth helix of the barrel. The structure of TGT in complex with preQ1 suggests a binding mode for tRNA where the phosphate backbone interacts with the zinc subdomain and the U33G34U35 sequence is recognized by the barrel. This model for tRNA binding is consistent with a base exchange mechanism involving a covalent tRNA-enzyme intermediate. This structure is the first example of a (beta/alpha)-barrel protein interacting specifically with a nucleic acid.     

Adaptive synthesis of distance fields

We address the computational resource requirements of 3D example-based synthesis with an adaptive synthesis technique that uses a tree-based synthesis map. A signed-distance field (SDF) is determined for the 3D exemplars, and then new models can be synthesized as SDFs by neighborhood matching. Unlike voxel synthesis approach, our input is posed in the real domain to preserve maximum detail. In comparison to straightforward extensions to the existing volume texture synthesis approach, we made several improvements in terms of memory requirements, computation times, and synthesis quality. The inherent parallelism in this method makes it suitable for a multicore CPU. Results show that computation times and memory requirements are very much reduced, and large synthesized scenes exhibit fine details which mimic the exemplars.     

Effect of water pretreatment on CO<Subscript>2</Subscript> capture using a potassium-based solid sorbent in a bubbling fluidized bed reactor

A bubbling fluidized bed reactor was used to study CO₂ capture from flue gas by using a potassium-based solid sorbent, sorbKX35 which was manufactured by the Korea Electric Power Research Institute. A dry sorbent, sorbKX35, consists of K₂CO₃ for absorption and supporters for mechanical strength. To increase initial CO₂ removal, some amount of H₂O was absorbed in the sorbent before injecting simulated flue gas. It was possible to achieve 100% CO₂ removal for more than 10 minutes at 60°C and a residence time of 2 s with H₂O pretreatment. When H₂O pretreatment time was long enough to convert K₂CO₃ of sorbKX35 into K₂CO₃ · 1.5H₂O, CO₂ removal was excellent. The results obtained in this study can be used as basic data for designing and operating a large scale CO₂ capture process with two fluidized bed reactors. This work was presented at the 6 ^th Korea-China Workshop on Clean Energy Technology held at Busan, Korea, July 4–7, 2006.     

Analysis of microbial adaptation at enzyme level for enhancing biodegradation rate of BTX

Catechol was found to be a common intermediate in the degradation of benzene and toluene byAlcaligenes xyhsoxidans Y234, and the ring cleavage of the catechol mediated by catechol 1,2-dioxygenase was a rate-determining step. Since benzene induced higher level of catechol 1,2-dioxygenase than toluene, the cells pre-adapted to benzene showed higher degradation rate of benzene and toluene. The degradation rate ofm-xylene was also increased significantly when benzene-adapted cells were inoculated.m-Xylene was metabolized via 3-methyl catechol which was effectively cleaved by catechol 1,2-dioxygenase.     

Investigation of point-to-point performance test of touch trigger probes on coordinate-measuring machines

A major factor contributing to the total measuring error of coordinate measuring machines (CMMs) is the performance of the probing sub-system. Probing test methods are typically used to detect errors due to the probing sub-system. The probe performance evaluation method specified in the ANSI B89 standard is investigated in this paper. The sampling plan associated in the probe performance evaluation was tested by using experimental probing data from a CMM. Research findings indicate that the performance of touch trigger probes is overestimated due to a systematic bias in the vertical direction of the best-fit reference ball center in the probe performance test. A two-latitude sampling plan synthesis method based on a pretravel model for touch trigger probes is proposed in this paper. The proposed method can be used to accurately identify the reference ball center in the performance test of touch trigger probes.     

Enhanced carrier confinement in AlInGaN-InGaN quantum wells in near ultraviolet light-emitting diodes

To increase carrier confinement, the GaN barrier layer was substituted with an AlInGaN quaternary barrier layer which was lattice-matched to GaN in the GaN-InGaN multiple quantum wells (MQWs). Photoluminescence (PL) and high-resolution X-ray diffraction measurements showed that the AlInGaN barrier layer has a higher bandgap energy than the originally used GaN barrier layer. The PL intensity of the five periods of AlInGaN-InGaN MQWs was increased by three times compared to that of InGaN-GaN MQWs. The electroluminescence (EL) emission peak of AlInGaN-InGaN MQWs ultraviolet light-emitting diode (UV LED) was blue-shifted, compared to a GaN-InGaN MQWs UV LED and the integrated EL intensity of the AlInGaN-InGaN MQWs UV LED increased linearly up to 100 mA. These results indicated that the AlInGaN-InGaN MQWs UV LED has a stronger carrier confinement than a GaN-InGaN MQWs UV LED due to the larger barrier height of the AlInGaN barrier layer compared to a GaN barrier layer.     

A study on a generalized parametric interpolator with real-time jerk-limited acceleration

The impossibility of exact arc length computation for ‘standard’ parametric curves such as Bézier/B-spline curves makes it difficult to generate a feedrate profile with desired accelerations in real-time. This paper presents a new recursive trajectory generation method that estimates an admissible path increment and determines the initiation of the final deceleration stage according to the distance left to travel estimated at every sampling time, resulting in exact feedrate trajectory generation through jerk-limited acceleration profiles for the parametric curves. The proposed approach allows the feedrate profile to be dynamically adjusted according to geometrical path constraints for the curved path. The simulation result has been also provided to illustrate the generation of smooth feedrate profile encompassing a sequence of mixed NC blocks including traditional linear and circular blocks.     

A New Clearance Design Method for Reciprocating Fuel Pumps of Medium-Speed Diesel Engines

A new clearance design method is proposed for a reciprocating fuel pump. Structural and hydrodynamic lubrication analyses were conducted to design the clearance of the pump, and the former was used to evaluate the clearance reduction due to the deformation that results from applying maximum supply pressure. The reduction in the maximum clearance was used as the limit of the design, and the two-dimensional Reynolds equation is used to evaluate the lubrication characteristics of the pump with variations in the viscosity and clearance. The optimal clearance in the pump must be separately designed in both the stem and the head because the lubrication characteristics and the deformation patterns of the two parts are different. The validity of the numerical model was then verified by determining that the position of the wear was also similar to that of the minimum film thickness. Moreover, a rig test system was also used to verify the clearance of the proposed design method.