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1.
This study investigated use and management of blogs in academic health sciences libraries. Quantitative and qualitative data were collected with a questionnaire distributed to bloggers and administrators representing 22 libraries self-identified as using blogs to publish library newsletters. Perceived barriers to use of blogs included lack of patron awareness and technical issues. Blogs were viewed as easy to use by librarians and blogs are used to promote licensed resources. Blogs do not replace other interactions with librarians, but in the future, could be used to develop a community, provide tools for learning, and increase visibility of the library. 相似文献
2.
Valeria Gaspari Camilla Ceccarani Marco Severgnini Gionathan Orioni Tania Camboni Luca Laghi Sara Morselli Claudio Foschi Antonella Marangoni Clarissa Consolandi Bianca Maria Piraccini 《International journal of molecular sciences》2022,23(10)
Background: Chlamydia trachomatis (CT) is the agent of the most common bacterial sexually transmitted infection worldwide. Until now, little information is available about the microbial composition of urine samples during CT urethritis. Therefore, in this study, we characterized the microbiome and metabolome profiles of first-void urines in a cohort of women with CT urethral infection attending an STI clinic. Methods: Based on CT positivity by nucleic acid amplification techniques on urine samples, the enrolled women were divided into two groups, i.e., “CT-negative” (n = 21) and “CT-positive” (n = 11). Urine samples were employed for (i) the microbiome profile analysis by means of 16s rRNA gene sequencing and (ii) the metabolome analysis by 1H-NMR. Results: Irrespective of CT infection, the microbiome of first-void urines was mainly dominated by Lactobacillus, L. iners and L. crispatus being the most represented species. CT-positive samples were characterized by reduced microbial biodiversity compared to the controls. Moreover, a significant reduction of the Mycoplasmataceae family—in particular, of the Ureaplasma parvum species—was observed during CT infection. The Chlamydia genus was positively correlated with urine hippurate and lactulose. Conclusions: These data can help elucidate the pathogenesis of chlamydial urogenital infections, as well as to set up innovative diagnostic and therapeutic approaches. 相似文献
3.
Albena Momchilova Roumen Pankov Alexander Alexandrov Tania Markovska Stefan Pankov Plamen Krastev Galya Staneva Evgenia Vassileva Nikolai Krastev Adriana Pinkas 《International journal of molecular sciences》2022,23(14)
Multiple sclerosis (MS) is an autoimmune, inflammatory, degenerative disease of the central nervous system. Changes in lipid metabolism have been suggested to play important roles in MS pathophysiology and progression. In this work we analyzed the lipid composition and sphingolipid-catabolizing enzymes in erythrocytes and plasma from MS patients and healthy controls. We observed reduction of sphingomyelin (SM) and elevation of its products—ceramide (CER) and shingosine (SPH). These changes were supported by the detected up-regulation of the activity of acid sphingomyelinase (ASM) in MS plasma and alkaline ceramidase (ALCER) in erythrocytes from MS patients. In addition, Western blot analysis showed elevated expression of ASM, but not of ALCER. We also compared the ratios between saturated (SAT), unsaturated (UNSAT) and polyunsaturated fatty acids and suggest, based on the significant differences observed for this ratio, that the UNSAT/SAT values could serve as a marker distinguishing erythrocytes and plasma of MS from controls. In conclusion, the application of lipid analysis in the medical practice would contribute to definition of more precise diagnosis, analysis of disease progression, and evaluation of therapeutic strategies. Based on the molecular changes of blood lipids in neurodegenerative pathologies, including MS, clinical lipidomic analytical approaches could become a promising contemporary tool for personalized medicine. 相似文献
4.
Capillary Network‐Like Organization of Endothelial Cells in PEGDA Scaffolds Encoded with Angiogenic Signals via Triple Helical Hybridization 下载免费PDF全文
Patrick J. Stahl Tania R. Chan Yu‐I Shen Guoming Sun Sharon Gerecht S. M. Yu 《Advanced functional materials》2014,24(21):3213-3225
Survival of tissue engineered constructs after implantation depends on proper vascularization. The differentiation of endothelial cells into mature microvasculature requires dynamic interactions between cells, scaffold, and growth factors, which are difficult to recapitulate in artificial systems. Previously, photocrosslinked poly(ethylene glycol) diacrylate (PEGDA) hydrogels displaying collagen mimetic peptides (CMPs), dubbed PEGDA‐CMP, that can be further conjugated with bioactive molecules via CMP‐CMP triple helix hybridization were reported. Here, it is shown that a bifunctional peptide featuring pro‐angiogenic domain mimicking vascular endothelial growth factor (VEGF) and a collagen mimetic domain that can fold into a triple helix conformation can hybridize with CMP side chains of the PEGDA‐CMP hydrogel, which results in presentation of insoluble VEGF‐like signals to endothelial cells. Presentation of VEGF‐like signals on the surface of micropatterned scaffolds in this way transforms cells from a quiescent state to elongated and aligned phenotype suggesting that this system could be used to engineer organized microvasculature. It is also shown that the pro‐angiogenic peptide, when applied topically in combination with modified dextran/PEGDA hydrogels, can enhance neovascularization of burn wounds in mice demonstrating the potential clinical use of CMP‐mediated matrix‐bound bioactive molecules for dermal injuries. 相似文献
5.
Metal Organic Framework Crystals in Mixed‐Matrix Membranes: Impact of the Filler Morphology on the Gas Separation Performance 下载免费PDF全文
Anahid Sabetghadam Beatriz Seoane Damla Keskin Nicole Duim Tania Rodenas Salman Shahid Sara Sorribas Clément Le Guillouzer Guillaume Clet Carlos Tellez Marco Daturi Joaquin Coronas Freek Kapteijn Jorge Gascon 《Advanced functional materials》2016,26(18):3154-3163
Mixed‐matrix membranes comprising NH2‐MIL‐53(Al) and Matrimid or 6FDA‐DAM have been investigated. The metal organic framework (MOF) loading has been varied between 5 and 20 wt%, while NH2‐MIL‐53(Al) with three different morphologies, nanoparticles, nanorods, and microneedles has been dispersed in Matrimid. The synthesized membranes have been tested in the separation of CO2 from CH4 in an equimolar mixture. At 3 bar and 298 K for 8 wt% MOF loading, incorporation of NH2‐MIL‐53(Al) nanoparticles leads to the largest improvement compared to nanorods and microneedles. The incorporation of the best performing filler, i.e., NH2‐MIL‐53(Al) nanoparticles, into the highly permeable 6FDA‐DAM has a larger effect, and the CO2 permeability increases up to 85% with slightly lower selectivities for 20 wt% MOF loading. Specifically, these membranes have a permeability of 660 Barrer with a CO2/CH4 separation factor of 28, leading to a performance very close to the Robeson limit of 2008. Furthermore, a new non‐destructive technique based on Raman spectroscopy mapping is introduced to assess the homogeneity of the filler dispersion in the polymer matrix. The MOF contribution can be calculated by modeling the spectra. The determined homogeneity of the MOF filler distribution in the polymer is confirmed by focused ion beam scanning electron microscopy analysis. 相似文献
6.
Hassan Artail Abdelkarim Ajami Tania Saouma Malak Charaf 《Wireless Communications and Mobile Computing》2016,16(14):1956-1971
This paper presents a faulty node detection approach for wireless sensor networks that aggregate measurement data on their way toward the sink (base station). The approach is based on the idea of commanding sensor nodes on the aggregation paths to temporarily stop including their readings in the received aggregated readings from their upstream neighbors. The scheme is dependent on the ability of the sink to detect faulty nodes through changes in the received aggregated readings at the sink using a Markov Chain Controller (MCC). The algorithm that is run in the sink uses the MCC to assign a state to each sensor node based on transitions that are triggered by receiving aggregated path readings, and accordingly deduces the nodes that may be faulty. The experimental results show at least 98% detection rate at the cost of reasonable detection delays and generated wireless network traffic. Copyright © 2016 John Wiley & Sons, Ltd. 相似文献
7.
Tania Bakshi David Pham Raminderjeet Kaur Bingyun Sun 《International journal of molecular sciences》2022,23(7)
N-Glycosylation (NG) and disulfide bonds (DBs) are two prevalent co/post-translational modifications (PTMs) that are often conserved and coexist in membrane and secreted proteins involved in a large number of diseases. Both in the past and in recent times, the enzymes and chaperones regulating these PTMs have been constantly discovered to directly interact with each other or colocalize in the ER. However, beyond a few model proteins, how such cooperation affects N-glycan modification and disulfide bonding at selective sites in individual proteins is largely unknown. Here, we reviewed the literature to discover the current status in understanding the relationships between NG and DBs in individual proteins. Our results showed that more than 2700 human proteins carry both PTMs, and fewer than 2% of them have been investigated in the associations between NG and DBs. We summarized both these proteins with the reported relationships in the two PTMs and the tools used to discover the relationships. We hope that, by exposing this largely understudied field, more investigations can be encouraged to unveil the hidden relationships of NG and DBs in the majority of membranes and secreted proteins for pathophysiological understanding and biotherapeutic development. 相似文献
8.
Drug repositioning, the approach of discovering different uses for existing drugs, has gained enormous popularity in recent years in the anticancer drug discovery field due to the increasing demand for anticancer drugs. Additionally, the repurposing of veterinary antiparasitic drugs for the treatment of cancer is gaining traction, as supported by existing literature. A prominent example is the proposal to implement the use of veterinary antiparasitics such as benzimidazole carbamates and halogenated salicylanilides as novel anticancer drugs. These agents have revealed pronounced anti-tumor activities and gained special attention for “double repositioning”, as they are repurposed for different species and diseases simultaneously, acting via different mechanisms depending on their target. As anticancer agents, these compounds employ several mechanisms, including the inhibition of oncogenic signal transduction pathways of mitochondrial respiration and the inhibition of cellular stress responses. In this review, we summarize and provide valuable information about the experimental, preclinical, and clinical trials of veterinary antiparasitic drugs available for the treatment of various cancers in humans. This review suggests the possibility of new treatment options that could improve the quality of life and outcomes for cancer patients in comparison to the currently used treatments. 相似文献
9.
Karthik Sathiyanadan Florian Alonso Sonia Domingos-Pereira Tania Santoro Lauriane Hamard Valrie Cesson Paolo Meda Denise Nardelli-Haefliger Jacques-Antoine Haefliger 《International journal of molecular sciences》2022,23(6)
Connexin37 (Cx37) and Cx40 form intercellular channels between endothelial cells (EC), which contribute to the regulation of the functions of vessels. We previously documented the participation of both Cx in developmental angiogenesis and have further shown that loss of Cx40 decreases the growth of different tumors. Here, we report that loss of Cx37 reduces (1) the in vitro proliferation of primary human EC; (2) the vascularization of subcutaneously implanted matrigel plugs in Cx37−/− mice or in WT using matrigel plugs supplemented with a peptide targeting Cx37 channels; (3) tumor angiogenesis; and (4) the growth of TC-1 and B16 tumors, resulting in a longer mice survival. We further document that Cx37 and Cx40 function in a collaborative manner to promote tumor growth, inasmuch as the injection of a peptide targeting Cx40 into Cx37−/− mice decreased the growth of TC-1 tumors to a larger extent than after loss of Cx37. This loss did not alter vessel perfusion, mural cells coverage and tumor hypoxia compared to tumors grown in WT mice. The data show that Cx37 is relevant for the control of EC proliferation and growth in different tumor models, suggesting that it may be a target, alone or in combination with Cx40, in the development of anti-tumoral treatments. 相似文献
10.
Scientometrics - The theme related to the oil production in the Brazilian territory is not limited to the extraction means, distribution and use of this product, and involves political, cultural... 相似文献