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排序方式: 共有1068条查询结果,搜索用时 15 毫秒
1.
Dr. Yibin Zhang Dr. Shuai Xia Shulin Wan Tessa E. Steenwinkel Tara Vohs Prof. Rudy L. Luck Prof. Thomas Werner Prof. Haiying Liu 《Chembiochem : a European journal of chemical biology》2021,22(13):2282-2291
Abnormal levels of glutathione, a cellular antioxidant, can lead to a variety of diseases. We have constructed a near-infrared ratiometric fluorescent probe to detect glutathione concentrations in biological samples. The probe consists of a coumarin donor, which is connected through a disulfide-tethered linker to a rhodamine acceptor. Under the excitation of the coumarin donor at 405 nm, the probe shows weak visible fluorescence of the coumarin donor at 470 nm and strong near-infrared fluorescence of the rhodamine acceptor at 652 nm due to efficient Forster resonance energy transfer (FRET) from the donor to the acceptor. Glutathione breaks the disulfide bond through reduction, which results in a dramatic increase in coumarin fluorescence and a corresponding decrease in rhodamine fluorescence. The probe possesses excellent cell permeability, biocompatibility, and good ratiometric fluorescence responses to glutathione and cysteine with a self-calibration capability. The probe was utilized to ratiometrically visualize glutathione concentration alterations in HeLa cells and Drosophila melanogaster larvae. 相似文献
2.
ABSTRACT Design and implementation of an effective dissemination programme for decentralised renewable energy system necessitate an accurate estimate of its utilisation potential. Hence, in this study, an attempt has been made to develop frameworks to estimate the utilisation potential of decentralised renewable energy systems in the state of Uttarakhand in India. Estimations imply large resource, technical and economic potentials of the domestic solar water heater, solar home system, solar lantern, family size biogas plant and improved biomass cookstove in Uttarakhand. With higher impact on the purchasing power of households, prevailing soft loan scheme has been found to be more appropriate than a capital subsidy for promoting the usage of decentralised renewable energy systems. 相似文献
3.
4.
A. Phillion H. W. Zurob C. R. Hutchinson H. Guo D. V. Malakhov J. Nakano G. R. Purdy 《Metallurgical and Materials Transactions A》2004,35(4):1237-1242
We have evaluated controlled decarburization as a method for probing the effect of alloying elements on ferrite growth from
austenite. The technique permits the exploration of longer-time ferrite layer growth; it minimizes the effects of interface
structure on ferrite growth; and it permits the isolation of the effects of temperature and alloying element concentration
on ferrite/austenite interface motion. The study of the decarburization of initially homogeneous Fe-C-Ni alloys was complemented
by experiments using specimens with a controlled nickel concentration gradient. Although the decarburization method yields
consistent results at longer times, it is found to be less appropriate for the study of initial ferrite growth. Nucleation
in the gas/solid interface region, coupled with uncertainties about the precise time of decarburization, leads to large relative
errors at the earliest times. For these reasons, the method is considered a valuable complement to studies based on precipitation
boundary conditions.
This article is based on a presentation given in the symposium “The Effects of Alloying Elements on the Gamma to Alpha Transformation
in Steels,” October 6, 2002, at the TMS Fall Meeting in Columbus, Ohio, under the auspices of the McMaster Centre for Steel
Research and the TMS-ASM Phase Transformations Committee. 相似文献
5.
A Notghi R Hutchinson D Kumar NB Smith LK Harding 《Canadian Metallurgical Quarterly》1994,35(7):976-981
Segmental colonic transit has been measured in 101 patients. Two MBq of 111Indium absorbed on resin pellets and encapsulated in an enteric coated capsule was given at 7 00 am. Hourly images during the first day, and three images during each subsequent day were acquired for up to three days. Using all scan and patient data the scans were categorised in one of the five patterns of colonic transit: normal, rapid, right delay, left delay, or generalised delay. The geometric centres and per cent activity at each time point was compared between the five groups of colonic transit patients to find the best time for imaging and so to distinguish the five groups. During the first day, early images did not help in diagnosis of patterns of transit, however, in the later images (six hours onwards after the ingestion of the activity) the rapid transit groups could be identified. Images at 27 and 51 hours were both required to distinguish all five groups of patients from each other. Only in the 'normal' transit patients was there some excretion of the activity during the course of the second day, otherwise there was no difference in the images taken in the course of a day (second or third day). A simplified protocol requires a minimum of three images to distinguish all five patterns of colonic transit. The activity should be ingested in the morning (7 00 am) and the first image taken at the end of the working day (8-10 hours after ingestion), the second image on the morning of the second day, and the third image during the course of the third day. This simple protocol would provide all the clinically relevant information necessary for correct classification of the colonic transit. 相似文献
6.
A 73-year-old HTLV-1 infected male developed overt ATL following 3-years observation, and the clonality of HTLV-1 infected cells changed before overt ATL onset. 10 micrograms of DNA, extracted from mononuclear cells was digested with PstI, and the clonal proliferation of HTLV-1 infected cells was examined by Southern blotting with LTR probe. 4 bands were observed 3 years prior to ATL onset, and 2 different bands were detected 2 years and 6 months later. This suggests that in some ATL cases, the clonality of monoclonally proliferated HTLV-1 infected cells may be changeable before overt ATL onset. 相似文献
7.
C. R. Hutchinson H. S. Zurob Y. Bréchet 《Metallurgical and Materials Transactions A》2006,37(6):1711-1720
The growth of allotriomorphic ferrite from austenite in Fe-C-X alloys is studied. Two systems have been selected: the Fe-C-Ni
system, in which the substitutional alloying element is expected to have a weak interaction with both the C and the moving
interface, and the Fe-C-Mo system, in which these interactions are expected to be non-negligible. The ferrite growth kinetics
was measured using two types of experiments: classical isothermal heat treatments and decarburization experiments. All of
the experimental observations can be quantitatively rationalized using a model that describes an evolution in interfacial
conditions from paraequilibrium (PE) to local equilibrium with negligible partitioning (LENP) during growth.
This article is based on a presentation made in the “Hillert Symposium on Thermodynamics & Kinetics of Migrating Interfaces
in Steels and Other Complex Alloys,” December 2–3, 2004, organized by the The Royal Institute of Technology in Stockholm,
Sweden. 相似文献
8.
Hong-hui YuJohn W Hutchinson 《Thin solid films》2003,423(1):54-63
Delamination of residually stressed thin film strips is analyzed to expose the dependence on strip width and film/substrate elastic mismatch. Isotropic films and substrates are assumed. The residual stress in the film is tensile and assumed to originate from mismatch due to thermal expansion or epitaxial deposition. Full and partial delamination modes are explored. In full delamination, the interface crack extends across the entire width of the strip and releases all the elastic energy stored in the strip as the crack propagates along the interface. The energy release rate available to propagate the interface crack is a strong function of the strip width and the elastic modulus of the film relative to that of the substrate. The energy release rate associated with full delamination is determined as a function of the interface crack length from initiation to steady-state, revealing a progression of behavior depending in an essential way on the three dimensionality of the strip. The dependence of the energy release rate on the remaining ligament as the interface crack converges with the strip end has also been calculated, and the results provide an effective means for inferring interface toughness from crack arrest position. A partial delamination propagates along the strip leaving a narrow width of strip attached to the substrate. In this case, the entire elastic energy stored in the strip is not released because the strain component parallel to the strip is not relaxed. A special application is also considered, in which a residually stressed metal superlayer is deposited onto a polymer strip. The energy release rate for an interface crack propagating along the interface between the polymer and the substrate is determined in closed form. 相似文献
9.
Compared attributions for negative wife behavior offered by 3 groups of husbands: 22 maritally violent and distressed, 17 nonviolent but maritally distressed, and 17 nonviolent and nondistressed. Husbands rated wife behaviors presented in 9 hypothetical problematic marital situation vignettes. On a measure of responsibility attributions, violent husbands were more likely than nondistressed husbands to attribute negative intentions, selfish motivation, and blame to the wife. On a measure of possible negative wife intentions, violent husbands were more likely than either distressed or nondistressed men to attribute negative intentions to the wife. Exploratory analyses suggested that certain types of marital situations (e.g., jealousy and rejection from wife) were particularly likely to elicit attributions of negative intent from violent husbands. (PsycINFO Database Record (c) 2010 APA, all rights reserved) 相似文献
10.
JJ Rankine DG Fortune CE Hutchinson DG Hughes CJ Main 《Canadian Metallurgical Quarterly》1998,23(15):1668-1676
STUDY DESIGN: Prospective comparative study of pain drawings with findings on lumbar spine magnetic resonance imaging. OBJECTIVES: To assess the ability of the pain drawing to predict the presence of nerve root compression. SUMMARY OF BACKGROUND DATA: Most research work has concentrated on the ability of the pain drawing to act as a screening method for psychological distress with less work directed at the influence the anatomic abnormality has on the pain drawing. METHODS: One hundred thirty-four consecutive outpatients attending for lumbar magnetic resonance imaging in the investigation of back and leg pain completed pain drawings and psychological testing immediately before the examination. The pain drawing was analyzed by previously reported criteria, and the magnetic resonance imaging was assessed independently for the presence of nerve compression by three radiologists. Multivariate stepwise discriminant analysis was used to identify patients with nerve compression on the basis of their pain drawing. RESULTS: Nerve compression was predicted by numbness in the anterolateral aspect of the foot. There was considerable overlap in the appearances of the pain drawings between patients with and without nerve compression, and the pain drawing correctly classified only 58% of patients with nerve compression. CONCLUSIONS: The pain drawing is not a good predictor of nerve compression on magnetic resonance imaging in a group of patients investigated for back and leg pain. It should be interpreted with caution and in light of the full clinical picture. 相似文献