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1.
Towards a Better Prediction of Cell Settling on Nanostructure Arrays—Simple Means to Complicated Ends
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Nina Buch‐Månson Sara Bonde Jessica Bolinsson Trine Berthing Jesper Nygård Karen L. Martinez 《Advanced functional materials》2015,25(21):3246-3255
Vertical arrays of nanostructures (NSs) are emerging as promising platforms for probing and manipulating live mammalian cells. The broad range of applications requires different types of interfaces, but cell settling on NS arrays is not yet fully controlled and understood. Cells are both seen to deform completely into NS arrays and to stay suspended like tiny fakirs, which have hitherto been explained with differences in NS spacing or density. Here, a better understanding of this phenomenon is provided by using a model that takes into account the extreme membrane deformation needed for a cell to settle into a NS array. It is shown that, in addition to the NS density, cell settling depends strongly on the dimensions of the single NS, and that the settling can be predicted for a given NS array geometry. The predictive power of the model is confirmed by experiments and good agreement with cases from the literature. Furthermore, the influence of cell‐related parameters is evaluated theoretically and a generic method of tuning cell settling through surface coating is demonstrated experimentally. These findings allow a more rational design of NS arrays for the numerous exciting biological applications where the mode of cell settling is crucial. 相似文献
2.
E Aurelius B Andersson M Forsgren B Sk?ldenberg O Stranneg?rd 《Canadian Metallurgical Quarterly》1994,170(3):678-681
Sequential samples of serum and cerebrospinal fluid (CSF), from 9 patients with herpes simplex encephalitis (HSE), were analyzed for cytokines and soluble cytokine receptors. The response to herpes simplex virus was characterized by a vigorous compartmentalized immune response. The intrathecal response comprised three different phases: an acute stage (first week of illness), characterized by elevated CSF levels of interleukin (IL)-6 and interferon-gamma; an early convalescence stage (weeks 2-6 after onset of disease), associated with peaking levels of tumor necrosis factor-alpha and late markers of the specific T cell-mediated immune response, soluble IL-2 receptor, and soluble CD8 antigen (sCD8); and finally, a late convalescence stage, lasting months to years and associated with persistently increased levels of sCD8 in particular. These findings show the compartmentalization and kinetics of the inflammatory response in HSE and demonstrate persistence of the intrathecal inflammatory process, which may have implications for antiviral and antiinflammatory therapy. 相似文献
3.
4.
The solubility of aluminum in NaF-AlF3-Al2O3 melts with various additives was found to increase with increasing NaF/AlF3 molar ratio (CR) and increasing temperature and to decrease with additions of A12O3, CaF2, MgF2, and LiF to the melts. With the use of literature data for the activities of NaF and A1F3 in cryolitic melts, three dissolution reaction models were found to give a good fit to the experimental solubility data.
According to the most probable of these models the total concentration of dissolved aluminum (aluminum and sodium species)
is given by cAl = cNa(diss) + cAlF2- + cAl2F3- + cAl3F4- + cAl4F5- In NaF rich melts, aluminum will dominantly dissolve as sodium, while at cryolite ratios commonly used in aluminum electrowinning
(CR = 2.25 to 2.7) the AlF
-2
-
-ion is the predominant dissolved metal species. Other species (A12F3
-, A13F4-, A14F5-) were found to be of some significance only in melts with high excess A1F3 (CR < 2). 相似文献
5.
The reversible persistent changes of the fill factor (FF) induced by the illumination and voltage bias along with changes in the electronic properties of the ZnO/CdS/Cu(In,Ga)Se2 photovoltaic devices have been studied. Admittance spectroscopy and capacitance–voltage characterization reveal a correlation between the FF and the space charge distribution within the absorber. Our experiments provide evidence that a major source of FF loss in efficient devices is caused by excess negative charge close to the interface. We explain the persistent changes in the net acceptor concentration in the interface region by the relaxation effects due to compensating donors—the same mechanism, which leads to metastable changes of the doping level in the bulk of the absorber. 相似文献
6.
Lysophosphatidylcholine (LPC) is formed by hydrolysis of PC in low density lipoprotein (LDL) and cell membranes by phospholipase A2 or by oxidation. Oxidized (ox) LDL activates endothelial cells, an effect mimicked by LPC. oxLDL also has the capacity to activate T and B cells, and antibody titers to oxLDL are related to the degree of atherosclerosis. The antigen in oxLDL responsible for its immune-stimulatory capacity is not well characterized, and we hypothesized that LPC was involved. We demonstrate herein the presence of antibodies against LPC, both of the IgG and IgM isotype, in 210 healthy individuals. This antibody reactivity was not specifically related to oxidation of the fatty acid moiety in LPC, since LPC containing only palmitic acid showed antibody titers equivalent to those of LPC containing unsaturated fatty acids. Antibody titers to PC were low compared with LPC, and hydrolysis of PC at the sn-2 position is thus essential for immune reactivity. There was a close correlation between anti-oxLDL and anti-LPC antibodies. Furthermore, LPC competitively inhibited anti-oxLDL reactivity, which indicates that LPC may explain a significant part of the immune-stimulatory properties of oxLDL. LPC, being a lipid, is not likely to be an antigen itself. Instead, LPC could form immunogenic complexes with peptides, which may induce and potentiate immune reactions in the vessel wall. This study adds to the evidence that LPC is an important component of oxLDL and emphasizes the potential role of phospholipase A2 in atherosclerosis. 相似文献
7.
Eva Plsgrd Carina Johansson Gang Li Geoff W. Grime J. T. Triffitt 《Nuclear instruments & methods in physics research. Section B, Beam interactions with materials and atoms》1997,130(1-4):431-438
To respond to varying environmental demands the bone tissue in the body is under continual reconstruction throughout life. It is known that metallic elements are important for maintaining normal bone structure, but their roles are not well understood. More information about the effects of metal excess or deficiency is needed to help in the development of metallic bone implants and to improve the treatment of bone fractures and defects. The Oxford Scanning Proton Microprobe (SPM) is being applied in two studies involving metal ions in bone: (1) bone regrowth and bonding to titanium bone implants may be influenced by diffusion of Ti ions into the bone. We are using microPIXE to determine the metal ion content of bone developing in contact with implants of pure Nb, Ti and Ti alloys. (2) Bone lengthening as a surgical procedure is induced by fracturing the bone and allowing it to heal with a small gap between the fractured ends created by the use of external fixators. The gap can be slowly increased during the healing process to stimulate the production of new bone. The enzymes and other constituents of the developing bone need certain metals for their function. Using experimental animals we have studied the concentrations of the metals and whether a deficiency of trace metals limits the optimum rate of bone lengthening. 相似文献
8.
The solubility of aluminum in NaF-AlF3-Al2O3 melts with various additives was found to increase with increasing NaF/AlF3 molar ratio (CR) and increasing temperature and to decrease with additions of A12O3, CaF2, MgF2, and LiF to the melts. With the use of literature data for the activities of NaF and A1F3 in cryolitic melts, three dissolution reaction models were found to give a good fit to the experimental solubility data. According to the most probable of these models the total concentration of dissolved aluminum (aluminum and sodium species) is given by cAl = cNa(diss) + cAlF2- + cAl2F3- + cAl3F4- + cAl4F5- In NaF rich melts, aluminum will dominantly dissolve as sodium, while at cryolite ratios commonly used in aluminum electrowinning (CR = 2.25 to 2.7) the AlF -2 - -ion is the predominant dissolved metal species. Other species (A12F3 -, A13F4-, A14F5-) were found to be of some significance only in melts with high excess A1F3 (CR < 2). 相似文献
9.
Kähkönen E Nykyri E Ilmarinen R Ketola R Lusa S Nygård CH Suurnäkki T 《Applied ergonomics》1992,23(3):186-190
A study was made of the appraisers' effect on the estimation of metabolic rate with the Edholm scale and a table of the ISO 7243 heat stress standard. The appraisers, five experienced and five inexperienced persons, estimated the metabolic rate of three different work tasks from videotapes. Analysis of variance indicated significant ( [Formula: see text] ) differences in the appraisers' recordings of the activities. The appraisers were grouped according to the similarity of the estimated values they gave. The groups thus contained both experienced and inexperienced appraisers, and it was not possible to classify the appraisers into experienced and inexperienced groups according to their earlier experience. The metabolic rates according to the Edholm scale were higher than according to the ISO 7243 table. The differences in metabolic rates given by the individual observers varied from 38 to 118 W/m(2). The variations in the estimation of metabolic rates were greater when the Edholm scale was used. This variation caused considerable variation also in the predicted mean vote, PMV index. It is recommended that the appraisers be selected carefully, because it is not possible to know whether a randomly selected appraiser is an 'average' or an 'extreme' appraiser without a test. Before conducting extensive field surveys where several appraisers estimate the metabolic rates, it would be useful to arrange training in order to calibrate the levels of the Edholm scale as well as ISO method among the appraisers because training clearly unified the estimation. 相似文献
10.
The poisoning effect of various halogen compounds on a nickel-based hydrogenation catalyst has been investigated. While alkyl
monochlorides did not affect the catalyst activity, alkyl monobromide and-iodide had a strong poisonous effect. Vicinal dichlorides,
1,1-dichlorides, and HC1 also poisoned the catalyst. It is shown that with chlorine containing compounds, the poisoning mechanism
involves a fission of the carbon-chlorine bond with the formation of HC1, while bromine and iodine compounds adsorb to the
catalyst surface as such in a simple equilibrium reaction. A mackerel oil previously shown to exhibit three kinetically distinguishable
poisoning effects has been further examined, and it is shown that its content of halogens can probably account for the observed
poisoning pattern. 相似文献