NMR-study of the stereochemistry and dynamic behaviour of thioacrylamides

Several model thioacrylamides, Me₂N CH = C(R) C(S) NMe₂ (R = H, Ph — 2a, b ) and (p)MeO C₆H₄ NH CHC(R) C(S) NMe₂ (R = H, Ph — 3a, b ) have been synthesized. The ¹H-n.m.r. spectra have been taken for discussing the E/Z-isomerism of the thioacrylamides and the assignment further strengthened by an added LIS-study. The 2-unsubstituted compounds have been found to exist exclusively in preferred configurations/conformations: 2a — E(s-cis); 3a — Z(s-cis); the 2-phenylsubstituted analogs are of divergent behaviour: in 2b the C(S) NMe₂ moiety is found to be twisted out of the common plane, and 3b prefers the flat s-trans conformations (E(s-trans) ⇌ Z(s-trans)). The compounds show restricted rotations about C¹, N- and C³, N-partial double bonds. The corresponding rotational barriers, determined as ΔG-values by dynamic n.m.r. spectroscopy, are discussed with respect to (i) the resonance branch at C¹, (ii) the influence of substituents, and (iii) the effect of steric hindrance in the thioacrylamide moiety generally.     

Studies on the mashing conditions of teff (Eragrostis tef) malt as a raw material for lactic acid‐fermented gluten‐free beverage

Formation of extracts and fermentable sugars during mashing can be limited by incomplete starch gelatinisation. The aim of this research was to develop mashing programme for 100% teff malt as a potential raw material for gluten‐free lactic acid‐fermented beverage. Isothermal mashing at temperatures ranging between 60 and 84 °C was conducted, and the highest extract (85%) was observed for the wort samples produced at temperatures higher than 76 °C. Sixty‐minute rest at 71 °C resulted in higher fermentable sugars than other tested conversion rest temperatures. Inclusion of lower mashing‐in temperature in the mashing programme also substantially improved the concentrations of free amino nitrogen (128 mg L^?1) and fermentable sugar (58 g L^?1) in the final wort. Therefore, 30‐min rest at 40 °C followed by 60‐min rest at 71 °C and 10‐min rest at 78 °C was found to be a suitable mashing programme for teff malt.     

Prevalence and antimicrobial susceptibility of Salmonella isolated from a variety of raw meat sausages in Gaborone (Botswana) retail stores

The objective of the study was to provide baseline data on the prevalence and antimicrobial susceptibility of Salmonella in different types of raw meat sausages directly accessible to the consumers in Gaborone, Botswana. A total of 300 raw sausages comprising 79 beef, 78 pork, 72 chicken, and 71 mutton samples were concurrently analyzed for the presence of Salmonella using a conventional culture method and a validated PCR method. The PCR assay results were in full concordance with those of the conventional culture method for the detection of Salmonella. Sixty-five (21.7%) of 300 samples were positive for Salmonella by both the conventional culture method and PCR assay. Even though more chicken samples contained Salmonella than did any other sausage type, the difference in the presence of Salmonella among the four sausages types was not significant. Eleven serotypes were identified, and Salmonella enterica subsp. salamae II was most prevalent in all the sausage types. Beef sausages generally had higher mesophilic bacterial counts than did the other three sausage types. However, higher microbial counts were not reflective of the presence of salmonellae. Susceptibility of the Salmonella enterica serotypes to 20 antimicrobial agents was determined, and Salmonella Muenchen was resistant to the widest array of agents and was mostly isolated from chicken sausages. Regardless of the meat of origin, all 65 Salmonella isolates were resistant to at least four antimicrobial agents: amikacin, gentamicin, cefuroxime, and tombramycin. This resistance profile group was the most common in all four sausage types, comprising 90% of all Salmonella isolates from beef, 71% from pork, 63% from mutton, and 35% from chicken. These results suggest that raw sausages pose a risk of transmitting multidrug-resistant Salmonella isolates to consumers.     

Decadal Analysis of River Flow Extremes Using Quantile-Based Approaches

Next to the traditional analysis of trends in time series of hydro-climatological variables, analysis of decadal oscillations in these variables is of particular importance for the risk assessment of hydro-climatological disasters and risk-based decision-making. Conventional parametric and nonparametric tests, however, need implementing a set of background assumptions related to serial structure and statistical distribution of data. They neither focus on the extreme events and their probability of occurrence. In order to get rid of these limitations, we suggest a modified version of the Sen Method (SM), combined with the Quantile Perturbation Method (QPM) for examining temporal variation of extreme hydrological events. The developed method is tested for decadal analysis of monthly and annual river flows at 10 hydrometric stations in the Qazvin plain in Iran. The results show oscillatory patterns in extreme river flow quantiles, with a positive anomaly during the 1990s and a negative one during the 2000s. It is also shown that the concurrent use of the two methods allows to set a complete picture on the temporal changes in high and low extremes in historical river flow observations in different seasons.     

Farmers’ perception of soil fertility change and their preferences for soil fertility management techniques for different land use types in Arsamma Watershed,Southwestern Ethiopian Highlands

This study aimed to assess farmers’ perception of soil fertility change and their priorities for soil fertility management techniques for different land use types in Arsamma Watershed, Southwestern Ethiopian Highlands. A three-stage random sampling technique was employed to select the sampled households. A smaller proportion of farmers perceived soil fertility decline in perennial cropland than in annual cropland as the farmers appeared to be less aware of below-ground processes of soil degradation. The farmers used physical, chemical and biological indicators to evaluate soil fertility changes. The rate of soil fertility decline and the occurrence of the contributory factors were land use specific. Increasing fertilizer requirement of croplands and decreasing yield per unit of land were the main indicators of soil fertility decline in annual and perennial cropland, respectively. Farmers’ preference for soil fertility management techniques was also found to depend on the type of land use. Integrated soil fertility management (ISFM) and agroforestry were the top priorities of the farmers, respectively, for annual and perennial cropland management. Farmers lack knowledge of below-ground processes of soil degradation by leaching, nutrient mining and soil acidity; and agricultural extension services should respond to this need. Further, ISFM for annual cropland and agroforestry for perennial cropland need to be encouraged in the study catchment.     

Effects of excessive speeding and falling asleep while driving on crash injury severity in Ethiopia: A generalized ordered logit model analysis

The severity of injury from vehicle crash is a result of a complex interaction of factors related to drivers’ behavior, vehicle characteristics, road geometric and environmental conditions. Knowing to what extent each factor contributes to the severity of an injury is very important. The objective of the study was to assess factors that contribute to crash injury severity in Ethiopia. Data was collected from June 2012 to July 2013 on one of the main and busiest highway of Ethiopia, which extends from the capital Addis Ababa to Hawassa. During the study period a total of 819 road crashes was recorded and investigated by trained crash detectors. A generalized ordered logit/partial proportional odds model was used to examine factors that might influence the severity of crash injury. Model estimation result suggested that, alcohol use (Coef. = 0.5565; p-value = 0.017), falling asleep while driving (Coef. = 1.3102; p-value = 0.000), driving at night time in the absence of street light (Coef. = 0.3920; p-value = 0.033), rainfall (Coef. = 0.9164; p-value = 0.000) and being a minibus or vans (Coef. = 0.5065; p-value = 0.013) were found to be increased crash injury severity. On the other hand, speeding was identified to have varying coefficients for different injury levels, its highest effects on sever and fatal crashes. In this study risky driving behaviors (speeding, alcohol use and sleep/fatigue) were a powerful predictor of crash injury severity. Therefore, better driver licensing and road safety awareness campaign complimented with strict police enforcement can play a pivotal role to improve road safety. Further effort needed as well to monitor speed control strategies like; using the radar control and physical speed restraint measures (i.e., rumble strips).     

Estimating sediment yield risk of reservoirs in northern Ethiopia using expert knowledge and semi‐quantitative approaches

Reservoir siltation is a serious problem that threatens the productivity and sustainability of water‐harvesting schemes. Quantification of sediment deposition in reservoirs and understanding of its major drivers are needed to apply targeted management interventions. Most of the techniques used to estimate sediment deposition in reservoirs require extensive measurements on a frequent time basis, as well as being costly and time‐consuming. Thus, a rapid and relatively economical means of assessing the erosion susceptibility of catchments and predicting their sediment yield potential is necessary. In this study, expert‐based rankings and semi‐quantitative factorial scoring approaches were applied to assess the siltation severity of 25 reservoirs in the Tigray region of northern Ethiopia. The results were then correlated with quantitative sediment yield estimates acquired for representative sites, and a sediment yield predication model was developed for the region. The calibrated model has an efficiency and relative root mean square error (RRMSE) of 79 and 36%, respectively, which is considered adequate to assess erosion susceptibility and siltation risk of reservoirs in similar environments. The study demonstrates that expert knowledge and rapid characterization of catchments, in terms of susceptibility to erosion, are viable options for assessing siltation risks and for analysing controlling factors at a larger number of sites, with minimum costs and acceptable accuracy.