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利用半导体激光器(LD)端面泵浦Nd:YVO_4和用KTP内腔倍频的绿激光器获得了120mW连续、稳定的532nm绿激光输出。泵浦阈值P_(th)=160mW,光-光转换效率η_s=8.1%。换用激光腔输出耦合镜,在不加KTP晶体倍频时,可连续输出1.06μm的红外激光,输出功率为450mW,光-光转换效率为31%。 相似文献
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将群桩筏板基础离散成桩和弹性板,桩筏基础与地基的共同作用转化为桩、弹性板与地基之间的力与位移协调分析。对不同的地基模型求解相应的地基柔度系数,同时将桩作为弹性杆件求解其柔度系数,并将筏板视为地基上的四边自由矩形板,求出其在地基反力、桩顶反力和外荷载以及简支边广义位移共同作用下的位移,最后通过力和位移协调建立桩、筏、地基之间的共同作用方程并求解。算例对比表明半解析半数值方法具有较好的精度,能满足实际工程计算需要。 相似文献
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A systematic approach was developed to derive non-stiff reduced mechanisms for direct numerical simulations (DNS) with explicit integration solvers. The stiffness reduction was achieved through on-the-fly elimination of short time-scales induced by two features of fast chemical reactivity, namely quasi-steady-state (QSS) species and partial-equilibrium (PE) reactions. The sparse algebraic equations resulting from QSS and PE approximations were utilized such that the efficiency of the dynamic stiffness reduction is high compared with general methods of time-scale reduction based on Jacobian decomposition. Using the dimension reduction strategies developed in our previous work, a reduced mechanism with 52 species was first derived from a detailed mechanism with 561 species. The reduced mechanism was validated for ignition and extinction applications over the parameter range of equivalence ratio between 0.5 and 1.5, pressure between 10 and 50 atm, and initial temperature between 700 and 1600 K for ignition, and worst-case errors of approximately 30% were observed. The reduced mechanism with dynamic stiffness removal was then applied in homogeneous and 1-D ignition applications, as well as a 2-D direct numerical simulation of ignition with temperature inhomogeneities at constant volume with integration time-steps of 5-10 ns. The integration was numerically stable and good accuracy was achieved. 相似文献
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Juanjuan Wang Yang Wang Tianfeng Lü Xia Yang Jing Liu Yang Dong Yinzheng Wang 《International journal of molecular sciences》2022,23(7)
The recent advent of single-cell RNA sequencing (scRNA-seq) has enabled access to the developmental landscape of a complex organ by monitoring the differentiation trajectory of every specialized cell type at the single-cell level. A main challenge in this endeavor is dissociating plant cells from the rigid cell walls and some species are recalcitrant to such cellular isolation. Here, we describe the establishment of a simple and efficient protocol for protoplast preparation in Chirita pumila, which includes two consecutive digestion processes with different enzymatic buffers. Using this protocol, we generated viable cell suspensions suitable for an array of expression analyses, including scRNA-seq. The universal application of this protocol was further tested by successfully isolating high-quality protoplasts from multiple organs (petals, fruits, tuberous roots, and gynophores) from representative species on the key branches of the angiosperm lineage. This work provides a robust method in plant science, overcoming barriers to isolating protoplasts in diverse plant species and opens a new avenue to study cell type specification, tissue function, and organ diversification in plants. 相似文献