全文获取类型
收费全文 | 3579篇 |
免费 | 225篇 |
国内免费 | 6篇 |
专业分类
电工技术 | 52篇 |
综合类 | 13篇 |
化学工业 | 714篇 |
金属工艺 | 132篇 |
机械仪表 | 53篇 |
建筑科学 | 180篇 |
矿业工程 | 5篇 |
能源动力 | 71篇 |
轻工业 | 704篇 |
水利工程 | 9篇 |
石油天然气 | 6篇 |
无线电 | 242篇 |
一般工业技术 | 571篇 |
冶金工业 | 416篇 |
原子能技术 | 24篇 |
自动化技术 | 618篇 |
出版年
2023年 | 57篇 |
2022年 | 74篇 |
2021年 | 117篇 |
2020年 | 98篇 |
2019年 | 95篇 |
2018年 | 102篇 |
2017年 | 79篇 |
2016年 | 114篇 |
2015年 | 109篇 |
2014年 | 131篇 |
2013年 | 181篇 |
2012年 | 166篇 |
2011年 | 199篇 |
2010年 | 166篇 |
2009年 | 132篇 |
2008年 | 138篇 |
2007年 | 113篇 |
2006年 | 95篇 |
2005年 | 75篇 |
2004年 | 59篇 |
2003年 | 49篇 |
2002年 | 51篇 |
2001年 | 35篇 |
2000年 | 30篇 |
1999年 | 45篇 |
1998年 | 124篇 |
1997年 | 80篇 |
1996年 | 49篇 |
1995年 | 39篇 |
1994年 | 23篇 |
1993年 | 25篇 |
1991年 | 19篇 |
1989年 | 21篇 |
1987年 | 20篇 |
1986年 | 19篇 |
1985年 | 29篇 |
1977年 | 18篇 |
1976年 | 25篇 |
1921年 | 21篇 |
1920年 | 30篇 |
1919年 | 22篇 |
1918年 | 27篇 |
1917年 | 84篇 |
1916年 | 45篇 |
1915年 | 43篇 |
1914年 | 39篇 |
1913年 | 68篇 |
1912年 | 115篇 |
1911年 | 31篇 |
1910年 | 31篇 |
排序方式: 共有3810条查询结果,搜索用时 15 毫秒
1.
Dr. Sandra Liebscher Dr. Sebastian Mathea Dr. Tobias Aumüller Dr. Andreas Pech Prof. Dr. Frank Bordusa 《Chembiochem : a European journal of chemical biology》2021,22(7):1201-1204
Fluorescent fusion proteins are powerful tools for studying biological processes in living cells, but universal application is limited due to the voluminous size of those tags, which might have an impact on the folding, localization or even the biological function of the target protein. The designed biocatalyst trypsiligase enables site-directed linkage of small-sized fluorescence dyes on the N terminus of integral target proteins located in the outer membrane of living cells through a stable native peptide bond. The function of the approach was tested by using the examples of covalent derivatization of the transmembrane proteins CD147 as well as the EGF receptor, both presented on human HeLa cells. Specific trypsiligase recognition of the site of linkage was mediated by the dipeptide sequence Arg-His added to the proteins’ native N termini, pointing outside the cell membrane. The labeling procedure takes only about 5 minutes, as demonstrated for couplings of the fluorescence dye tetramethyl rhodamine and the affinity label biotin as well. 相似文献
2.
Simon Neumann Pin Hu Felix Bretschneider Holger Schmalz Andreas Greiner 《大分子材料与工程》2021,306(7):2100090
In this study, blends of the bio-based poly(limonene carbonate) (PLimC) with different commodity polymers are investigated in order to explore the potential of PLimC toward generating more sustainable polymer materials by reducing the amount of petro- or food-based polymers. PLimC is employed as minority component in the blends. Next to the morphology and thermal properties of the blends the impact of PLimC on the mechanical properties of the matrix polymers is studied. The interplay of incompatibility and zero-shear melt viscosity contrast determines the blend morphology, leading for all blends to a dispersed droplet morphology for PLimC. Blends with polymers of similar structure to PLimC (i.e., aliphatic/aromatic polyester) show the best performance with respect to mechanical properties, whereas blends with polystyrene or poly(methyl methacrylate) are too brittle and polyamide 12 blends show very low elongations at break. In blends with Ecoflex (poly(butylene adipate-co-terephthalate)) and Arnitel EM400 (copoly(ether ester)) with poly(butylene terephthalate) hard and polytetrahydrofuran soft segments) a threefold increase in E-modulus can be achieved, while keeping the elongation at break at reasonable high values of ≈200%, making these blends highly interesting for applications. 相似文献
3.
Dr. Raysa Khan Tareque Dr. Storm Hassell-Hart Dr. Tobias Krojer Dr. Anthony Bradley Dr. Srikannathasan Velupillai Dr. Romain Talon Dr. Michael Fairhead Dr. Iain J. Day Kamlesh Bala Dr. Robert Felix Dr. Paul D. Kemmitt Prof. Paul Brennan Prof. Frank von Delft Dr. Laura Díaz Sáez Prof. Kilian Huber Prof. John Spencer 《ChemMedChem》2020,15(24):2513-2520
Combined photochemical arylation, “nuisance effect” (SNAr) reaction sequences have been employed in the design of small arrays for immediate deployment in medium-throughput X-ray protein–ligand structure determination. Reactions were deliberately allowed to run “out of control” in terms of selectivity; for example the ortho-arylation of 2-phenylpyridine gave five products resulting from mono- and bisarylations combined with SNAr processes. As a result, a number of crystallographic hits against NUDT7, a key peroxisomal CoA ester hydrolase, have been identified. 相似文献
4.
5.
Evangelos Pavlakis Michelle Neumann Thorsten Stiewe 《International journal of molecular sciences》2020,21(24)
Tumor progression to a metastatic and ultimately lethal stage relies on a tumor-supporting microenvironment that is generated by reciprocal communication between tumor and stromal host cells. The tumor–stroma crosstalk is instructed by the genetic alterations of the tumor cells—the most frequent being mutations in the gene Tumor protein p53 (TP53) that are clinically correlated with metastasis, drug resistance and poor patient survival. The crucial mediators of tumor–stroma communication are tumor-derived extracellular vesicles (EVs), in particular exosomes, which operate both locally within the primary tumor and in distant organs, at pre-metastatic niches as the future sites of metastasis. Here, we review how wild-type and mutant p53 proteins control the secretion, size, and especially the RNA and protein cargo of tumor-derived EVs. We highlight how EVs extend the cell-autonomous tumor suppressive activity of wild-type p53 into the tumor microenvironment (TME), and how mutant p53 proteins switch EVs into oncogenic messengers that reprogram tumor–host communication within the entire organism so as to promote metastatic tumor cell dissemination. 相似文献
6.
As a result of shrinking fossil fuels, biomass as a regenerative energy source gains importance. To realize biomass projects it is essential to investigate in convenient thermal procedures. On this evidence an analysis and evaluation of diverse gasification technologies with different boundary conditions and diverse biomasses are indispensable. Form and kind of the biomass as well as the type of the gasification plant cause different compositions of the product gas. The gasifiers show advantages and disadvantages concerning the biomass and the produced gas quality, depending on reactor type, kind of heat supply, gasification medium, and the pressure ratio in the reactor. As the ideal gasifier for different biomass is presently not available, it will be shown, which biomass is suitable for fixed bed or fluidized bed gasifiers. 相似文献
7.
8.
G Berding H Schliephake G Neumann R Schmelzeisen FW Neukam GJ Meyer KF Gratz H Hundeshagen 《Canadian Metallurgical Quarterly》1996,35(5):156-163
AIM: The aim of this study was early differentiation between uncomplicated and complicated processes of healing in the jaw using bone SPECT. METHODS: Investigations were performed in 40 mandibular fractures and 26 jaws after onlay osteoplasty as well as secondary insertion of implants. Bone SPECT was carried out within 1-2 months and after approximately 4-5 months. The uptake in the jaw was assessed semi-quantitatively using ROI analysis. RESULTS: Fractures with uncomplicated healing showed a decrease of uptake in follow-up, whereas fractures with an infection in the later course showed an increase, resulting in a significantly higher uptake at the follow-up investigation for the latter group. 1-2 months after onlay osteoplasty significantly lower uptake was found in regions with later occurrence of sequestration. In regions with implants in which osseointegration failed, there was significant reduction of uptake initially and significant elevation at the follow-up investigation. CONCLUSION: These results indicate a prognostic relevance of bone SPECT in the evaluation of processes of healing in the jaw. 相似文献
9.
用多种快重离子辐照高定向石墨(HOPG),借助扫描隧道显微镜(STM)系统地研究了表面及体内缺陷,结果表明,离子在表面及解理面上都形成了小丘状的缺陷,且在表面较容易形成,可以用非连续损伤径迹结构来对其进行解释。 相似文献
10.
Alpidem, an imidazopyridine that acts at the gamma-aminobutyric acid/benzodiazepine receptor complex, has been reported to be an effective anxiolytic with a more favorable side effect profile than benzodiazepines. The effect of alpidem was investigated in an 8-week, open, clinical trial in 13 patients with panic disorder, with or without agoraphobia. Three patients were responders (much improved or very much improved), five patients were nonresponders, and five patients dropped out after less than 6 weeks of treatment. Significant improvement was seen in the sample as a whole for spontaneous panic attacks, phobic avoidance, and anticipatory anxiety. Most improvement occurred during the first 4 weeks of treatment, and responders had milder panic disorder at baseline. Adverse effects were generally mild. After 8 weeks of treatment, taper of medication over 2 weeks occurred without significant worsening of panic disorder symptoms. The efficacy of alpidem in the treatment of panic disorder remains uncertain and requires assessment in a controlled trial. 相似文献