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1.
Antibody microarrays are gaining popularity as a high-throughput technology to investigate the proteome. However, protein extracts from most body fluid or biopsy samples are available in very small volumes and are often unsuitable for large-scale antibody microarray studies. To demonstrate the potential for protein analysis with as little as a few nanoliters of sample, we have developed a new technology called NanoProbeArrays based on piezoelectric liquid dispensing for non-contact printing and probing of antibody arrays. Instead of flooding the protein sample on the antibody microarray surface, as in conventional microarray screening, a piezoelectric inkjet printer is used to dispense nanoliters of fluorescently labeled proteins over the antibody spots on the array. The ability of NanoProbeArrays to precisely identify and reliably distinguish between test proteins from different sources, without any loss of sensitivity and specificity as compared with conventional antibody microarrays, is illustrated here. The utility of NanoProbeArrays for biomarker identification in a complex biological sample was tested by detecting the cytokine interleukin-4 in serum. The significant reduction in volume of sample during NanoProbeArray analysis, as compared with conventional antibody microarrays, offers new opportunities for basic and applied proteomic research. 相似文献
2.
Widely used electron beam welding machines are equipped with heavy power supplies, located in a special oil tank and connected with an electron gun to a high-voltage cable. A special system detects electric discharges in the electron gun space, which may arise during welding and then it tries to switch off the high voltage to interrupt an electric arc. Such disadvantages have been eliminated with the novel power supply described here. The Q of resonance circuit of this supply is stabilized and as a result circulating power appears. During an electric discharge in the gun, power is not sent to the electron gun but circulates between the electronic parts of the resonance circuit without losses and “waits” for the break in the short circuit. The power supply is much smaller and lighter than supplies of similar rating used nowadays. It is connected directly to the electron gun chamber without a high-voltage cable. The first construction of the supply was designed for electron beam welding machine of 5 kW power and 60 kV accelerating voltage. Tests of the new power supply in laboratory and industrial conditions have shown its usefulness for electron beam welding. 相似文献
3.
Katarzyna Matkiewicz Agnieszka Bukowska Wiktor Bukowski 《Journal of Inorganic and Organometallic Polymers and Materials》2012,22(2):332-341
Hydroxyl functionalized gel type resins derived from 2-hydroxyethyl, 2-hydroxypropyl and 2-hydroxy-3-phenoxypropyl methacrylates
were applied to covalent immobilization of Mn(salen) complexes via glutarate and carbonate linkers. The obtained catalysts
were characterized by FTIR and DR UV–Vis methods and elemental analysis. It was found that the activity of polymeric catalysts
obtained in this way depended on both the nature of the functional and cross-linking monomers and the degree of the cross-linking
of polymer matrices and the way of complex immobilization. The highest activity was observed for the complexes immobilized
on the HEMA resins cross-linked with 3%-mol of diethylene glycol dimethacrylate. For instance, in the presence of the catalysts
with (S,S)-salen ligand S-epoxystyrene and (1S, 2R)-epoxyindene could be obtained with 46% and 69% ee, respectively. Attempts
have also been made to recycle the selected catalyst. However, a rapid fall off in the activity was observed, although the
selectivity was similar in which three cycles. 相似文献
4.
T. Tsvetkova S. BalabanovE. Skordeva S. KitovaJ. Sielanko D. MaczkaJ. Zuk 《Vacuum》2003,72(2):143-147
The effects of ion implantation and subsequent annealing on the surface morphology of thin amorphous chalcogenide films of the As-Se system have been studied. Ion implantation of nitrogen (N+) with an energy E=100 keV and high doses (typically D=1.1016 cm−2) has been carried out at room temperature. Subsequent thermal annealing treatments near the melting temperature (Tg∼160°C) have been performed leading to changes in the thin-film morphology, which are dependent on the As content and are best expressed for the As3Se2 films. Optical microscope photographs reveal details of the rippled surface exhibiting quasi-regular domain-like structure. The parameter λ, describing it, is dependent on the film thickness and grows with its increase. Thermal annealing near Tg also influences the diffuse optical reflectivity—it increases considerably while the interference features tend to disappear with the appearance of the rippled film surface structure and the effect is again greater for the thicker films. 相似文献
5.
6.
The partial eigenfunction expansion (PEE) method combined with the classical finite difference frequency domain (FDFD) algorithm is proposed to accelerate frequency domain analysis of waveguide components. Examples are shown validating the method both for eigenvalue and deterministic problems. 相似文献
7.
Die Bestimmung des Grenzumformgrades eines Stahles, der während der plastischen Verformung nicht überschritten werden kann, ist von großer Bedeutung. Die vorliegende Arbeit berichtet über Ergebnisse von Warmtorsionsversuchen, aus denen der Grenzumformgrad als Funktion von Temperatur und Umformgeschwindigkeit ermittelt wurde. Die Versuche wurden an den Stählen X 10 CrNiTi 18 9, 10 CrMo 9 10, X 10 CrNiMoTi 18 12, X 20 CrMoV 12 1 und X 2 NiCrNb 25 18 durchgeführt. 相似文献
8.
Laboratoriumsuntersuchungen über die Reduktionskinetik von Eisenerzen in der Wirbelschicht. Identifizierung des geschwindigkeitsbestimmenden Teilschritts. Ableitung der kinetischen Prozeßgleichung. Bestimmung der Modellparameter. Besprechung der präsentierten verallgemeinerten Prozeßgleichung. 相似文献
9.
A fast finite difference frequency domain (FDFD) formulation for obtaining resonant frequencies of structures with perturbed rotational symmetry is described. The formulation combines a three-dimensional (3-D) eigenfunction function expansion algorithm with 3-D FDFD in the cylindrical coordinate system. Numerical tests have showed that the technique gives high accuracy results and significant CPU time reduction. 相似文献
10.