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排序方式: 共有373条查询结果,搜索用时 15 毫秒
1.
Katja F. Hellendahl Felix Kaspar Dr. Xinrui Zhou Zhaoyi Yang Prof. Dr. Zhen Huang Prof. Dr. Peter Neubauer Dr. Anke Kurreck 《Chembiochem : a European journal of chemical biology》2021,22(11):2002-2009
Selenium-modified nucleosides are powerful tools to study the structure and function of nucleic acids and their protein interactions. The widespread application of 2-selenopyrimidine nucleosides is currently limited by low yields in established synthetic routes. Herein, we describe the optimization of the synthesis of 2-Se-uridine and 2-Se-thymidine derivatives by thermostable nucleoside phosphorylases in transglycosylation reactions using natural uridine or thymidine as sugar donors. Reactions were performed at 60 or 80 °C and at pH 9 under hypoxic conditions to improve the solubility and stability of the 2-Se-nucleobases in aqueous media. To optimize the conversion, the reaction equilibria in analytical transglycosylation reactions were studied. The equilibrium constants of phosphorolysis of the 2-Se-pyrimidines were between 5 and 10, and therefore differ by an order of magnitude from the equilibrium constants of any other known case. Hence, the thermodynamic properties of the target nucleosides are inherently unfavorable, and this complicates their synthesis significantly. A tenfold excess of sugar donor was needed to achieve 40−48 % conversion to the target nucleoside. Scale-up of the optimized conditions provided four Se-containing nucleosides in 6–40 % isolated yield, which compares favorably to established chemical routes. 相似文献
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软件定义网络的发展使得其对信息安全的要求越来越高,匿名通信成为软件定义网络的关键研究点.由于软件定义网络特有的管控分离性能,传统网络中的匿名方案难以在其平台应用.提出一种新的软件定义网络匿名通信方案,通过重写数据包实现其匿名性,通过扩展Open Flow协议实现数据包的正常转发,并且采用新的最大值随机路径算法保证通信路径的隐匿性,实现基于软件定义网络的低延迟、高匿名传输. 相似文献
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由恒流二极管串联分压组成的直流辅助电源 总被引:3,自引:3,他引:0
设计了一种由恒流模块和稳压模块串联组成的直流辅助电源。介绍了直流辅助电源电路的工作原理,并设计了由恒流模块和稳压模块串联组成的直流辅助电源样机,测试了样机在电网电压波动及负载变化时的输出电压情况。测试结果表明:接入220 V交流电源时,样机可以稳定输出12 V电压,其性能满足设计要求。 相似文献
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水下光声图像配准是水下设备实现信息融合的关键技术。在简述了水下光声图像配准的概念及实例的基础上,分析了目前水下光声图像重建与复原的相关算法,详细综述了水下异源图像基于区域和特征的配准算法研究进展,重点论述了基于图像域和形状特征相似度的两个准确度较高的研究方向的发展现状,并根据其他领域的异源图像配准的研究热点,从增加成像模型的结构性约束、引入相位一致性和生成对抗网络等算法提高配准精度,展望了水下声光图像配准研究的发展趋势。 相似文献
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The application of high-frame-rate cameras as well as the complex image processing techniques will lead to a series of problems, such as high system cost and long transmission delay. In this paper, we introduce narrow-band filtering technology to reduce the impact of optical noise and reduce the complexity of image processing from the physical level. We also introduce multiple-input multiple-output (MIMO) technology into the optical camera communication (OCC) system to increase system transmission rate, and propose a light emitting diode (LED) array decoding algorithm based on the directional projection method to reduce the system delay. By accumulating the target pixel values in each row and column of the image, the proposed method then determines the position and boundary of the detected target to distinguish the target area from the background. Experimental results indicate that the communication distance can reach up to 5.5 m without error bits detected. When the LED array at the transmitter of this system flashes at a frequency of 12 Hz, the transmission rate can reach 126.32 bit/s. 相似文献
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典型相关分析(CCA)作为一种经典的特征融合方法,广泛用于模式识别领域,其目标是学习相关投影方向使两组变量间的相关性最大,但其没有考虑样本的类标签信息和样本间的信息冗余(MDOCCA),从而影响了融合后特征的监督敏感性和鉴别力。为此,该文提出一种标签敏感的多重集正交相关特征融合方法,该方法在典型相关分析理论基础上,将类标签信息嵌入到特征融合框架,同时加入正交约束确保融合特征最大限度的不相关,减少特征信息冗余,提高鉴别力。在不同图像数据集上的实验结果显示该方法是一种有效的特征融合方法。 相似文献
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Tribological performance of filled PTFE‐based friction material for ultrasonic motor under different temperature and vacuum degrees
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In this work, a glass fiber reinforced polytetrafluoroethylene (PTFE)‐based friction material with good properties for ultrasonic motor was fabricated. The effects of temperature and vacuum degree on the tribological behavior of the PTFE‐based friction material were investigated; the evolutions of friction‐wear modes and mechanisms were also discussed as function of temperature and vacuum degree. The results show that the delamination and fatigue wear are predominant under the effects of repeated shearing and dynamic contact under atmospheric environment. While wear mechanisms change from adhesive to abrasive and fatigue wear as it is cooled from 30 to ?60 °C at vacuum environment. Under high vacuum, adhesive wear was prone to taking place at room temperature for high frictional heat which increased the wear rate and extended the running‐in period. Experiment shows that the highest no‐load speed, output power, and holding torque of ultrasonic motor at room temperature under atmospheric environment are 220 rpm, 9.9 W, and 1.21 N m, respectively. © 2017 Wiley Periodicals, Inc. J. Appl. Polym. Sci. 2017 , 134 , 45358. 相似文献