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基于无荧光的螺环结构与具有荧光的开环酰胺的平衡反应,本文合成了一个能在水基的缓冲溶液中选择性地识别Cu^2+的罗丹明衍生物FD2.当在HEPES缓冲溶液中加入10当量的Cu^2+时,FD2的单光子激发荧光和双光子激发荧光的强度均表现出明显的增强;更为重要的是,运用双光子荧光显微技术可以选择性地对活细胞内Cu^2+进行成像. 相似文献
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The layer-by-layer complexation technique of polymer and metal ion was successfully utilized to fabricate the uhrathin muhilayer film of poly(3-thiophene acetic acid (PTAA) and Tb^3 ion by dipping the substrates alternatively in polymer and Tb^3 ion aqueous solutions. UV - vis measurement revealed that the absorbance has linearity with the bilayer number from layer to layer and the X-ray photoelectron spectrum (XPS) confirmed the existence of Tb^3 ion. The pH of both the polymer and TbCl3 solutions influence the thickness dramatically while the concentration of the solutions is not so sensitive. The luminescent spectrum of the complex film shows the characteristic emission of Tb^3 ion as well as the ligand indicating the formation of the complex. 相似文献
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十三陵蓄能电站使用UEA膨胀混凝土作压力管道钢衬回填混凝土,混凝土具有传力结构的性能并可承担外水压力的作用,效果良好。 相似文献
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