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1.
Mucsi Zoltan; Gaspari Zoltan; Orosz Gyorgy; Perczel Andras 《Protein engineering, design & selection : PEDS》2003,16(9):673-681
Three peptides modelling a highly potent, 35-residue chymotrypsininhibitor (Schistocerca gregaria chymotrypsin inhibitor) weredesigned and synthesized by convergent peptide synthesis. Foreach model peptide, the inhibitory constant (Ki) on chymotrypsinand the solution structure were determined. In addition, moleculardynamics calculations were performed for all of them. Two modelscontaining approximately half of the parent inhibitor (17 of35 residues) were designed and subsequently found to have nosubstantial inhibitory activity (Ki values in the mM range).The third model composed of 24 amino acid residues proved tobe an effective (Ki 107) inhibitor of bovine chymotrypsin.Both the solution structure properties determined by NMR spectroscopyand the dynamic behaviour of the latter model system are comparableto the native inhibitor. In contrast, the structure and dynamicsof the first two related model peptides show characteristicdifferences. We suggest that the conformation and flexibilityof the modelled protease inhibitor are crucial for its biologicalefficiency. Moreover, the structural and dynamic features ofthe binding loop (2833) and those of the rest of themolecule appear to be interdependent. Most importantly, thesestructural characteristics can be rationally modified, at leastpartially, by peptide design. Received March 7, 2003; revised August 25, 2003; accepted August 26, 2003. 相似文献
2.
Benjamin Zeiss Andras Kovacs Nikolay Pakulin Bogdan Stanca-Kaposta 《International Journal on Software Tools for Technology Transfer (STTT)》2014,16(3):285-294
With more than 10 years of maturing through industrial use and standardization, the Testing and Test Control Notation (TTCN-3) has become a widely used technology that many businesses depend upon for ensuring their product quality. With the rising number of supporters and tools, the demand for a means to assess the standards compliance of TTCN-3 tools has increased. In this article, we describe the motivation, approach, methodology, and results of the still ongoing project to develop a standardized conformance test suite for TTCN-3 tools. We discuss the challenges involved in creating such a test suite, the way to deal with imposed resource limitations of the project, and where we think the effort is heading. 相似文献
3.
Andras Ferencz Erik G. Learned-Miller Jitendra Malik 《International Journal of Computer Vision》2008,77(1-3):3-24
Object identification is a specialized type of recognition in which the category (e.g. cars) is known and the goal is to recognize
an object’s exact identity (e.g. Bob’s BMW). Two special challenges characterize object identification. First, inter-object
variation is often small (many cars look alike) and may be dwarfed by illumination or pose changes. Second, there may be many
different instances of the category but few or just one positive “training” examples per object instance. Because variation
among object instances may be small, a solution must locate possibly subtle object-specific salient features, like a door
handle, while avoiding distracting ones such as specular highlights. With just one training example per object instance, however,
standard modeling and feature selection techniques cannot be used. We describe an on-line algorithm that takes one image from
a known category and builds an efficient “same” versus “different” classification cascade by predicting the most discriminative
features for that object instance. Our method not only estimates the saliency and scoring function for each candidate feature,
but also models the dependency between features, building an ordered sequence of discriminative features specific to the given
image. Learned stopping thresholds make the identifier very efficient. To make this possible, category-specific characteristics
are learned automatically in an off-line training procedure from labeled image pairs of the category. Our method, using the
same algorithm for both cars and faces, outperforms a wide variety of other methods. 相似文献
4.
5.
Tiffany D. Andras Troy S. Alexander Asiri Gahlena R. Mitchell Parry Facundo M. Fernandez Julia Kubanek May D. Wang Mark E. Hay 《Journal of chemical ecology》2012,38(10):1203-1214
Coral reefs are in global decline, with seaweeds increasing as corals decrease. Although seaweeds inhibit coral growth, recruitment, and survivorship, the mechanism of these interactions is poorly understood. Here, we used field experiments to show that contact with four common seaweeds induces bleaching on natural colonies of Porites rus. Controls in contact with inert, plastic mimics of seaweeds did not bleach, suggesting seaweed effects resulted from allelopathy rather than shading, abrasion, or physical contact. Bioassay-guided fractionation of the hydrophobic extract from the red alga Phacelocarpus neurymenioides revealed a previously characterized antibacterial metabolite, neurymenolide A, as the main allelopathic agent. For allelopathy of lipid-soluble metabolites to be effective, the compounds would need to be deployed on algal surfaces where they could transfer to corals on contact. We used desorption electrospray ionization mass spectrometry (DESI-MS) to visualize and quantify neurymenolide A on the surface of P. neurymenioides, and we found the molecule on all surfaces analyzed, with highest concentrations on basal portions of blades. 相似文献
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7.
In today's digital electronic integrated circuits device heating is one of the most critical issues. Overheating can cause failures in functionality and device malfunction. In certain circumstances overheating of ICs can cause physical destruction of the device itself. This paper introduces a solution to determine cell and gate heating curves across the standard cell IC's surface. The presented methodology and toolset is tightly integrated into standardized logic simulator engines thus providing digital circuit designers a low-level, cell-resolution temperature distribution map during logic simulations. Actual temperatures of each consisting cell of the design can be monitored throughout the whole logic simulation. By being able to monitor temperatures of digital cells during initial simulations, it allows us to detect hot-spots and overheating caused malfunctions far before manufacture. By using the spatial location and temperature magnitude of hot-spots acquired from the presented methodology, place and route (P&R) tools can be driven to change cell placement and routing in order to avoid heating caused failures. Additionally, cooling solutions can be developed using the simulated temperature maps of the IC's surface. This paper also presents various aspects of power characterization methods which were used throughout the experiments. 相似文献
8.
Ground-based full-sky imaging polarimetry of rapidly changing skies and its use for polarimetric cloud detection 总被引:3,自引:0,他引:3
For elimination of the shortcomings of imaging polarimeters that take the necessary three pictures sequentially through linear-polarization filters, a three-lens, three-camera, full-sky imaging polarimeter was designed that takes the required pictures simultaneously. With this polarimeter, celestial polarization patterns can be measured even if rapid temporal changes occur in the sky: under cloudy sky conditions, or immediately after sunrise or prior to sunset. One of the possible applications of our polarimeter is the ground-based detection of clouds. With use of the additional information of the degree and the angle of polarization patterns of cloudy skies measured in the red (650 nm), green (550 nm), and blue (450 nm) spectral ranges, improved algorithms of radiometric cloud detection can be offered. We present a combined radiometric and polarimetric algorithm that performs the detection of clouds more efficiently and reliably as compared with an exclusively radiometric cloud-detection algorithm. The advantages and the limits of three-lens, three-camera, full-sky imaging polarimeters as well as the possibilities of improving our polarimetric cloud detection method are discussed briefly. 相似文献
9.
Design tool integration is a highly relevant area of software engineering that can greatly improve the efficiency of development processes. Design patterns have been widely recognized as important contributors to the success of software systems. This paper describes and compares two large-grain, architectural design patterns that solve specific design tool integration problems. Both patterns have been implemented and used in real-life engineering processes. 相似文献
10.