A bacteriophage reagent for Salmonella: molecular studies on Felix 01

Felix 01 (F01) is a bacteriophage originally isolated by Felix and Callow which lyses almost all Salmonella strains and has been widely used as a diagnostic test for this genus. Molecular information about this phage is entirely lacking. In the present study, the DNA of the phage was found to be a double-stranded linear molecule of about 80 kb. 11.5 kb has been sequenced and in this region A + T content is 60%. There are relatively few restriction endonuclease cleavage sites in the native genome and clones show this is due to their absence rather than modification. A restriction map of the genome has been constructed. The ends of the molecule cannot be ligated although they contain 5' phosphates. At least 60% of the genome must encode proteins. In the sequenced portion, many open reading frames exist and these are tightly packed together. These have been examined for homology to published proteins but only 1 to 17 shows similarity to known proteins. F01 is therefore the prototype of a new phage family. On the basis of restriction sites, codon usage and the distribution of nonsense codons in the unused reading frames, a strong case can be made for natural selection that reacts to mRNA structure and function.     

Dolichol-mediated enhanced protein N-glycosylation in experimental diabetes--a possible additional deleterious effect of hyperglycemia

The role of estrogen and the estrogen receptor (ER) in the induction and promotion of tumors was investigated by using transgenic MT-mER mice, which overexpress the ER. It was hypothesized that because of this abnormal expression of the ER, the reproductive-tract tissues of the MT-mER mice may be more susceptible to tumors after neonatal exposure to the potent synthetic estrogen diethylstilbestrol (DES). Normally non-estrogen responsive tissues that may have expressed ER as a result of the transgene were also studied for DES-induced tumors. Wild-type and MT-mER littermates were treated with 2 micrograms/pup/d DES 1-5 d after birth and then killed at 4, 8, 12, and 18 mo of age. The DES-treated MT-mER mice demonstrated a significantly higher incidence of uterine adenocarcinoma at 8 mo (73%) than the DES-treated wild-type mice (46%). The tumors of the MT-mER mice were often more aggressive than those in the wild-type animals. These tumors were also preceeded at 4 mo by a significantly higher incidence of the preneoplastic lesion atypical hyperplasia in the MT-mER mice (26% compared with 0% in the wild-type mice). Other DES-induced abnormalities were observed at equal rates in the wild-type and MT-mER mice. Although no tumors were observed in untreated wild-type females, a single untreated MT-mER female had uterine adenocarcinoma at 18 mo. These data indicate that the level of ER present in a tissue may also be a determining factor in development of estrogen-responsive tumors.     

Adult age differences in episodic memory: Further support for an associative-deficit hypothesis.

This study further tested an associative-deficit hypothesis (ADH; M. Naveh-Benjamin, 2000), which attributes a substantial part of older adults' deficient episodic memory performance to their difficulty in merging unrelated attributes-units of an episode into a cohesive unit. First, the results of 2 experiments replicate those observed by M. Naveh-Benjamin (2000) showing that older adults are particularly deficient in memory tests requiring associations. Second, the results extend the type of stimuli (pictures) under which older adults show this associative deficit. Third, the results support an ADH in that older adults show less of an associative deficit when the components of the episodes used are already connected in memory, thereby facilitating their encoding and retrieval. Finally, a group of younger adults who encoded the information under divided-attention conditions did not show this associative deficit. (PsycINFO Database Record (c) 2010 APA, all rights reserved)     

Effective moduli of multi-scale composites

The effective moduli of a multi-scale composite are evaluated by a bottom-up (hierarchical) modeling approach. We focus on a two-scale structure in which the small scale includes a platelet array inside a matrix, and the large scale contains fibers inside a composite matrix. We demonstrate that the principal moduli of the multi-scale composite can be fine-tuned by the platelet arrangement and orientation. As a case study, we consider the phenomenon of fiber micro-buckling within the multi-scale composite. It is found that the compressive micro-buckling strength can be considerably increased for specific platelet orientations. The multi-scale design approach presented here can be used to generate novel families of composite materials with tunable mechanical properties.     

Efficient Slide Attacks

The slide attack, presented by Biryukov and Wagner, has already become a classical tool in cryptanalysis of block ciphers. While it was used to mount practical attacks on a few cryptosystems, its practical applicability is limited, as typically, its time complexity is lower bounded by \(2^n\) (where n is the block size). There are only a few known scenarios in which the slide attack performs better than the \(2^n\) bound. In this paper, we concentrate on efficient slide attacks, whose time complexity is less than \(2^n\). We present a number of new attacks that apply in scenarios in which previously known slide attacks are either inapplicable, or require at least \(2^n\) operations. In particular, we present the first known slide attack on a Feistel construction with a 3-round self-similarity, and an attack with practical time complexity of \(2^{40}\) on a 128-bit key variant of the GOST block cipher with unknown S-boxes. The best previously known attack on the same variant, with known S-boxes (by Courtois), has time complexity of \(2^{91}\).     

A programming approach to mathematics

This article presents an attempt to employ a programming approach to mathematical formalization. Six self-instructional units (120 instructional hours) have been developed for the Open University in Israel. This material was tried on three different types of populations: (a) bright high school students (11th–12th grade, A-level mathematics), (b) mathematics teachers with a programming background, and (c) university students studying mathematics, natural sciences and engineering. The results indicated that the programming approach is complementary to high school and university mathematics. The opportunity to explore, the algorithmic approach to mathematical operations and the visualization of mathematical objects and processes facilitates mathematical understanding and insights. It was most beneficial to the majority of the students who perform on a moderate level of abstraction. The course was less advantageous to those who have a very abstract way of perceiving mathematics or to those who are interested in mathematics only on a technical level. However, the vast majority of students gained new ways of understanding mathematical topics such as functions, curves, probability, game theory, recursive functions, envelopes, tiling, curve fitting, etc. They gained, as well, new strategies of using microcomputers to solve and further explore many mathematical problems which they could not solve before.     

Stress intensity factor calculation for a modified round bend bar by 3-D finite element analysis

Stress intensity factors were calculated for a modified round bend bar (MRBB) using 3-D finite element analysis. The results were compared with published solutions for a rectangular and round bend bars. The stress intensity values for the MRBB are between those for the two other geometries as expected. The stress intensity solutions were non-dimensionalized utilizing limit solution for short and long cracks.     

The biomechanics of the locust ovipositor valves: a unique digging apparatus

The female locust has a unique mechanism for digging in order to deposit its eggs deep in the ground. It uses two pairs of sclerotized valves to displace the granular matter, while extending its abdomen as it propagates underground. This ensures optimal conditions for the eggs to incubate and provides them with protection from predators. Here, the direction-dependent biomechanics of the locust''s major, dorsal digging valves are quantified and analysed under forces in the physiological range and beyond, considering the hydration level as well as the females'' sexual maturation state. Our findings reveal that the responses of the valves to compression forces in the digging and propagation directions change upon sexual maturation to follow their function and depend on environmental conditions. In addition, mature females, which lay eggs, have stiffer valves, up to approximately 19 times the stiffness of the pre-mature locusts. The valves are stiffer in the major working direction, corresponding to soil shuffling and compression, compared with the direction of propagation. Hydration of the valves reduces their stiffness but increases their resilience against failure. These findings provide mechanical and materials guidelines for the design of novel non-drilling burrowing tools, including three-dimensionally printed anisotropic materials based on composites     

A meeting with clay: Individual narratives, self-reflection, and action.

The arts-based qualitative research presented in this article explores the interaction between nonartists and a specific art material, clay, and on reflection, the meaning that they make from this interaction. The aspect of meaning making focused on relates to a typology of thinking and doing strategies that emerged from the research. Thinking and doing are seen as 2 aspects of a creative process here with clay, which are interdependent. The thinking category encompasses 4 groups in which the participants employ different modes of thinking strategies. Different modes of thinking create a different kind of doing with the material. These "dialogues" between creator and material can be seen to exemplify individual ways of "making sense" in the interaction between sensing, feeling, thinking, and doing; and the material, in which structure, process, content, and meaning intertwine. (PsycINFO Database Record (c) 2010 APA, all rights reserved)