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1.
A General Role for Early Onset Cues and Intra-Event Learning; Comment on McDonald and Siegel (2004).
Research on classical conditioning with drug unconditional stimuli has had a profound effect on the understanding of general conditioning processes. The experiment reported by R. V. McDonald and S. Siegel (see record 2004-10475-001) demonstrates that cues coincident with the onset of an event can become associated with the rest of the event. This sort of learning is probably ubiquitous and has been proposed as a mechanism behind the development of panic disorder, in which interoceptive cues coincident with the start of a panic attack can be associated with the rest of the attack and can eventually come to elicit full-blown panic on their own. Evidence that extinction exposure to early onset cues can reduce their power is especially important. Drug conditioning research continues to provide a powerful testing ground for important general principles of learning. (PsycINFO Database Record (c) 2010 APA, all rights reserved) 相似文献
2.
G. Gregoris F. Bouton C. De Keukeleire P. Siliprandi F. Baio L. De Schepper W. De Ceuninck L. Tielemans T. Ahrens M. Krumm 《Quality and Reliability Engineering International》1996,12(4):247-252
We present the results of SHORTEST, a BRITE EURAM II project for the introduction in industry of an advanced approach to the reliability evaluation of electronic assemblies. The achievements so far are the development and validation of seven in-situ test techniques. Five of the test techniques demonstrate the capability of detecting failure mechanisms at 48 hours with moderate stress conditions, and half of the in-situ test results obtained at 48 hours on 10 subtechnologies correlate with conventional tests. All correlation results are presented. The application of the method for quality assurance and building-in reliability is discussed. The final aim of SHORTEST is to look for the exploitation of the developed in-situ techniques. The marketing study that will define the industrial needs of European companies is presented. In parallel, the insertion in the CECC specification of recommendations based on the SHORTEST principle is foreseen. 相似文献
3.
Bouton Y Guyot P Beuvier E Tailliez P Grappin R 《International journal of food microbiology》2002,75(1-2):27-38
In order to obtain a better understanding of the biochemical events taking place in Saccharomyces cerevisiae during the lag phase, the proteins expressed during the first hours after inoculation were investigated by two-dimensional (2-D) gel electrophoresis and compared to those expressed in late respiratory growth phase. The studies were performed on a haploid strain (S288C) grown in defined minimal medium. Some of the abundant proteins, whose expression relative to total protein expression was induced during the lag phase, were identified by MALDI MS, and the expression of the corresponding genes was assessed by Northern blotting. The rate of protein synthesis was found to increase strongly during the lag phase and the number of spots detected on 2-D gels increased from 502 spots just after inoculation to 1533 spots at the end of the lag phase. During the first 20 min, the number of detectable spots was considerably reduced compared to the number of spots detected from the yeast in respiratory growth just prior to harvest and inoculation (747 spots), indicating an immediate pausing or shutdown in synthesis of many proteins just after inoculation. In this period, the cells got rid of most of their buds. The MALDI MS-identified, lag phase-induced proteins were adenosine kinase (Ado1p), whose cellular role is presently uncertain, cytosolic acetaldehyde dehydrogenase (Ald6p) and (DL)-glycerol-3-phosphatase 1, both involved in carbohydrate metabolism, a ribosomal protein (Asc1p), a fragment of the 70-kDa heat shock protein Ssb1, and translationally controlled tumour protein homologue (Yk1056cp), all involved in translation, and S-adenosylmethionine synthetase I involved in biosynthesis reactions. The level of mRNA of the corresponding genes was found to increase strongly after inoculation. By pattern matching using previously published 2-D maps of yeast proteins, several other lag phase-induced proteins were identified. These were also proteins involved in carbohydrate metabolism, translation, and biosynthesis reactions. The identified proteins together with other, yet unidentified, lag phase-induced proteins are expected to be important for yeast growth initiation and could be valuable biological markers for yeast performance. Such markers would be highly beneficial in the control and optimisation of industrial fermentations. 相似文献
4.
D De Vos C Bouton A Sarniguet P De Vos M Vauterin P Cornelis 《Canadian Metallurgical Quarterly》1998,180(24):6551-6556
The sequence of oprI, the gene coding for the major outer membrane lipoprotein I, was determined by PCR sequencing for representatives of 17 species of rRNA group I pseudomonads, with a special emphasis on Pseudomonas aeruginosa and Pseudomonas fluorescens. Within the P. aeruginosa species, oprI sequences for 25 independent isolates were found to be identical, except for one silent substitution at position 96. The oprI sequences diverged more for the other rRNA group I pseudomonads (85 to 91% similarity with P. aeruginosa oprI). An accumulation of silent and also (but to a much lesser extent) nonsilent substitutions in the different sequences was found. A clustering according to the respective presence and/or positions of the HaeIII, PvuII, and SphI sites could also be obtained. A sequence cluster analysis showed a rather widespread distribution of P. fluorescens isolates. All other rRNA group I pseudomonads clustered in a manner that was in agreement with other studies, showing that the oprI gene can be useful as a complementary phylogenetic marker for classification of rRNA group I pseudomonads. 相似文献
5.
Six experiments with rat subjects examined the effect of yohimbine, an alpha-2 adrenergic autoreceptor antagonist, on the extinction of conditioned fear to a tone. Experiments 1 and 2 demonstrated that systemic administration of yohimbine (1.0 mg/kg) facilitated a long-term decrease in freezing after extinction, and this depended on pairing drug administration with extinction training. However, Experiments 3 and 4 demonstrated that yohimbine did not eradicate the original fear learning: Freezing was renewed when the tone was tested outside of the extinction context. Experiments 5 and 6 found that the contextually specific attenuation of fear produced by yohimbine transferred to another extinguished conditional stimulus (CS) and not to a nonextinguished CS. The results suggest that yohimbine, when administered in the presence of a neutral context, creates a form of inhibition in that context that allows that specific context to reduce fear of an extinguished CS. (PsycINFO Database Record (c) 2010 APA, all rights reserved) 相似文献
6.
Four experiments with rats examined partial reinforcement in appetitive conditioning. In Experiment 1, adding nonreinforced trials to a continuous reinforcement schedule slowed acquisition, whereas deleting reinforcers did not. Trial massing suppressed performance and learning. In Experiment 2, conditioning with a short conditioned stimulus (CS) was rapid, and partial reinforcement with a short CS was as effective as continuous reinforcement with equal accumulated time in the CS. In Experiment 3, conditioning was nevertheless influenced by the probability of reinforcement. In Experiments 3 and 4, conditioning was especially disrupted when nonreinforced trials preceded reinforced trials closely in time. The results underscore the importance of temporal variables in conditioning but are more consistent with trial-based accounts than time-accumulation accounts of conditioning. (PsycINFO Database Record (c) 2010 APA, all rights reserved) 相似文献
7.
The protein tyrosine phosphatase PTP-PEST displays remarkable substrate specificity, in vitro and in vivo for p130cas a signalling intermediate implicated in mitogenic signalling, cell-adhesion induced signalling, and in transformation by a variety of oncogenes. We have identified a high affinity interaction between the SH3 domain of p130cas and a proline-rich sequence (P335PPKPPR) within the C-terminal segment of PTP-PEST. Mutation of proline 337 within this sequence to alanine significantly impairs the ability of PTP-PEST to recognise tyrosine phosphorylated p130cas as a substrate, without qualitatively affecting the selectivity of the interaction. Thus the highly specific nature of the interaction between PTP-PEST and p130cas appears to result from a combination of two distinct substrate recognition mechanisms; the catalytic domain of PTP-PEST contributes specificity to the interaction with p130cas, whereas the SH3 domain-mediated association of p130cas and PTP-PEST dramatically increases the efficiency of the interaction. Furthermore, our results indicate that one important function of the p130cas SH3 domain is to associate with PTP-PEST and thereby facilitate the dephosphorylation of p130cas, resulting in the termination of tyrosine phosphorylation-dependent signalling events downstream of p130cas. 相似文献
8.
9.
This paper is a report of methods of balancing wear and traction in passenger tire treads with lithium catalyzed polymers. The results demonstrate the versatility of the alkyllithium system for preparing a wide variety of polymers and copolymers with differing wear and traction properties in passenger tire treads. In addition, the use of this system for preparing polymers with improved processing and green strength is briefly discussed. 相似文献
10.
A pressure-heat treatment, which disrupts the myofibrillar structure of meat but leaves the connective tissues essentially intact, was used to compare the connective tissue component of toughness in the Semimembranosus and Longissimus dorsi muscles from nine Brahman cross and nine buffalo steers, 24 to 29 months of age. For assessment of samples, peak force, initial yield force and peak force minus initial yield force values were determined from Warner-Bratzler shear force-deformation curves.
In the control, non-pressure-heat treated samples, the only breed difference detected was in peak minus initial yield force value, which was significantly lower for the beef Semimembranosus muscles. However, for the pressure-heat treated samples of both muscles, peak force and peak minus initial yield force values were significantly lower for beef than for buffalo. The pressure-heat treatment could thus be used to detect differences in the contribution of connective tissue to toughness which would otherwise be obscured by the differences in the myofibrillar toughness. 相似文献