The effect of penicillin on fatty acid synthesis and excretion inStreptococcus mutans BHT

Treatment of exponentially growing cultures ofStreptococcus mutans BHT with growth-inhibitory concentrations (0.2 μg/ml) of benzylpenicillin stimulates the incorporation of [2-¹⁴C] acetate into lipids excreted by the cells by as much as 69-fold, but does not change the amount of¹⁴C incorporated into intracellular lipids. At this concentration of penicillin cellular lysis does not occur. The radioactive label is incorporated exclusively into the fatty acid moieties of the glycerolipids. The increase in the radioactive content of the extracellular lipids reflects an actual net increase in the total fatty acid content as determined by a chemical assay. During a 4-hr incubation in the presence of penicillin, the extracellular fatty acid ester concentration (per mg cell dry weight) increases 1.5 fold, even though there is no growth or cellular lysis. No change is observed in the intracellular fatty acid ester content. An indication of the relative rate of fatty acid synthesis was most readily obtained by placingS. mutans BHT in a buffer containing¹⁴C-acetate. Under these nongrowing conditions free fatty acids are the only lipids labeled, a factor which simplifies the assay. The addition of glycerol to the buffer causes all of the nonesterified fatty acids to be incorporated into glycerolipid. The cells excrete much of the lipid whether glycerol is present or not. Addition of penicillin to the nongrowth supporting buffer system does not stimulate the incorporation of [¹⁴C]-acetate into fatty acids. However, if cells are exposed to penicillin in a growth-supporting medium and then are transferred to the nongrowing buffer system containing no penicillin, the previously exposed cells retain the ability to incorporate [¹⁴C]-acetate into fatty acid at a higher rate than untreated cells over a prolonged period of time. The stimulation of [¹⁴C]-acetate into fatty acids in this system parallels but is not dependent on the stimulation by penicillin of the incorporation of [¹⁴C]-glycerol into glycerolipid and lipoteichoic acid synthesis previously demonstrated by our laboratory. The material of this paper is part of a thesis to be submitted by J.L.B. in partial fulfillment of the requirements for the Ph.D. degree from the Department of Biochemistry, Temple University.     

Behaviour and Performance of a Water Resource System in Québec (Canada) Under Adapted Operating Policies in a Climate Change Context

The behaviour of the water resource system of the Peribonka River (Quebec, Canada) exploited for hydropower is evaluated under various hydrological regimes, using different climate change scenarios. The hydrological regime of the recent past and the regimes of 30 climate projections are considered. The potential hydrological regimes are simulated for climate projections from five general circulation models (GCM) for two greenhouse gas emission scenarios and three temporal horizons (2020, 2050 and 2080). For each hydrological regime, weekly reservoir operating rules are calculated with a dynamic and stochastic optimization model. Simulations of the water resource system with adapted operating rules in these climate change contexts are compared with the management of the water resource system at the control period (1961–1990). For the majority of climate projections, the analysis of simulations in the context of climate change shows an increase in hydropower and in annual unproductive spills. These increases reach 22% and 300%, respectively, compared to the control period. Also, the reliability of a reservoir is compromised for half of the climate projections, with annual probabilities reaching above the maximum operating levels, up to 0.3%, whereas these probabilities were null for the control period. Despite the rise in production, the annual efficiency of the power plants would fluctuate between −5 to +8%, depending on the power plant, the climate projection and the horizon.     

Optimal Hydropower Generation Under Climate Change Conditions for a Northern Water Resources System

This paper examines climate change impacts on the water resources system of the Manicouagan River (Québec, Canada). The objective is to evaluate the performance of existing infrastructures under future climate projections and the associated uncertainties. The main purpose of the water resources system is hydropower production. A reservoir optimization algorithm, Sampling Stochastic Dynamic Programming (SSDP), was used to derive weekly operating decisions for the existing system subject to reservoir inflows reflecting future climate, for optimum hydropower production. These projections are simulations from the SWAT hydrologic model for climate change scenarios for the period from 2010 to 2099. Results show that the climate change will alter the hydrological regime of the study area: earlier timing of the spring flood, reduced spring peak flow, and increased annual inflows volume in the future compared to the historical climate. The SSDP optimization algorithm adapted the operating policy to the future hydrological regime by adjusting water reservoir levels in the winter and spring, and increasing the release through turbines, which in the end increased power generation. However, there could be more unproductive spills for some power plants, which would decrease the overall efficiency of the existing water resources system.     

Structural and Non-Structural Climate Change Adaptation Strategies for the Péribonka Water Resource System

This paper discusses the possibility for a privately managed hydro-power system to adapt to a projected increase in water flow in their central-Québec watersheds by adding power generation potential. Runoffs simulated by a lumped rainfall-runoff model were fed into a stochastic dynamic programming (SDP) routine to generate reservoir operating rules. These rules were optimized for maximum power generation under maximal and minimal reservoir level constraints. With these optimized rules, a power generation simulator was used to predict the amount of generated hydropower. The same steps, excluding calibration, were performed on 60 climate projections (from 23 general circulation models and 3 greenhouse gas emission scenarios) for future horizons 2036–2065 and 2071–2100. Reservoir operation rules were optimized for every climate change projection for the 3 power plants in the system. From these simulations, it was possible to determine hydropower numbers for both horizons. The same steps were performed under a modified system in which an additional turbine was added to each power plant. Results show that both the non-structural (optimizing reservoir rules) and structural (adding turbines) adaptation measures allow for increased power production, but that adapting operating rules is sufficient to reap the most of the benefits of increased water availability.     

Adaptation to Climate Change in the Management of a Canadian Water-Resources System Exploited for Hydropower

The management adaptation potential of the Peribonka River water resource system (Quebec, Canada) is investigated in the context of the evolution of climate change. The objective of this study is to evaluate the impacts on hydropower, power plant efficiency, unproductive spills and reservoir reliability due to changes in the hydrological regimes. The climate change projections used here are from the Canadian regional climate model (CRCM) nested by the Canadian-coupled global climate model (CGCM3) forced with the SRES A2 greenhouse gas emission scenario. The hydrological regimes were simulated with the distributed hydrological model Hydrotel. They were incorporated into a dynamic and stochastic optimization model in order to adapt the operating rules of the water resource system annually, according to the evolution of the climate. The impacts were analyzed over the years 1961–2099, split into four periods for comparison purposes: control period (1961–1990), horizon 2020 (2010–2039), horizon 2050 (2040–2069) and horizon 2080 (2070–2099). The main results indicate that annual mean hydropower would decrease by 1.8% for the period 2010–2039 and then increase by 9.3% and 18.3% during the periods 2040–2069 and 2070–2099, respectively. The trend to increase is statistically significant starting from 2061 (Mann–Kendall with p = 5%). The change in the mean annual production is statistically significant for the 2040–2069 and 2070–2099 periods (t-test with p = 5%). Also, the change in the variance is significant for the periods 2010–2039, 2040–2069 and 2070–2099 (F-test). Annual mean unproductive spills would increase from 1961–2099, but the trend is not statistically significant. However, the changes in the variance of the annual mean spills are significant in the periods 2010–2039, 2040–2069 and 2070–2099. Overall, the reliability of a reservoir would decrease and the vulnerability increase as the climate changes.     

The role of optimism in social network development, coping, and psychological adjustment during a life transition.

The authors investigated the extent to which social support and coping account for the association between greater optimism and better adjustment to stressful life events. College students of both genders completed measures of perceived stress, depression, friendship network size, and perceived social support at the beginning and end of their 1st semester of college. Coping was assessed at the end of the 1st semester. Greater optimism, assessed at the beginning of the 1st semester of college, was prospectively associated with smaller increases in stress and depression and greater increases in perceived social support (but not in friendship network size) over the course of the 1st semester of college. Mediational analyses were consistent with a model in which increases in social support and greater use of positive reinterpretation and growth contributed to the superior adjustment that optimists experienced. (PsycINFO Database Record (c) 2010 APA, all rights reserved)     

Unusual mechanism of oxygen atom transfer and product rearrangement in the catalytic reaction of 2-methyl-3-hydroxypyridine-5-carboxylic acid oxygenase

The oxygenation reaction of 2-methyl-3-hydroxypyridine-5-carboxylic acid (MHPC) oxygenase with the substrate, MHPC, was investigated. Two oxygenated flavin intermediates C(4a)-hydroperoxy flavin and C(4a)-hydroxy flavin were found, implying that the enzyme functions similarly to flavoprotein hydroxylases. This finding is supported by the results of independent oxygen-18 tracer experiments, which showed that one atom of oxygen from 18O2 and one atom of oxygen from H218O are incorporated in the product. MHPC oxygenase normally catalyzes both the oxygenation and the hydrolytic ring opening of the pyridine ring of MHPC to yield the acyclic compound, alpha-(N-acetylaminomethylene)succinic acid. Using 5-hydroxynicotinic acid (5HN), which has no 2-methyl group, we tested whether the hydrolytic reaction was due to the presence of the 2-methyl group on MHPC (that prevented rearomatization of the initial product) or to the specific properties of MHPC oxygenase. Product analysis of the enzymatic reaction of 5HN and MHPC oxygenase shows that the enzyme catalyzes the hydroxylation and subsequent hydrolysis of the hydroxylated substrate to yield an acyclic product. The investigation of the oxygenation reaction demonstrates that the enzyme uses the same mechanism to catalyze the 5HN reaction as it does in the MHPC reaction.     

MFG-E8 Reduces Aortic Intimal Proliferation in a Murine Model of Transplant Vasculopathy

Transplant vasculopathy is characterized by endothelial apoptosis, which modulates the local microenvironment. Milk fat globule epidermal growth factor 8 (MFG-E8), which is released by apoptotic endothelial cells, limits tissue damage and inflammation by promoting anti-inflammatory macrophages. We aimed to study its role in transplant vasculopathy using the murine aortic allotransplantation model. BALB/c mice were transplanted with fully mismatched aortic transplants from MFG-E8 knockout (KO) or wild type (WT) C57BL/6J mice. Thereafter, mice received MFG-E8 (or vehicle) injections for 9 weeks prior to histopathological analysis of allografts for intimal proliferation (hematoxylin and eosin staining) and leukocyte infiltration assessment (immunofluorescence). Phenotypes of blood leukocytes and humoral responses were also evaluated (flow cytometry and ELISA). Mice receiving MFG-E8 KO aortas without MFG-E8 injections had the most severe intimal proliferation (p < 0.001). Administration of MFG-E8 decreased intimal proliferation, especially in mice receiving MFG-E8 KO aortas. Administration of MFG-E8 also increased the proportion of anti-inflammatory macrophages among graft-infiltrating macrophages (p = 0.003) and decreased systemic CD4⁺ and CD8⁺ T-cell activation (p < 0.001). An increase in regulatory T cells occurred in both groups of mice receiving WT aortas (p < 0.01). Thus, the analarmin MFG-E8 appears to be an important protein for reducing intimal proliferation in this murine model of transplant vasculopathy. MFG-E8 effects are associated with intra-allograft macrophage reprogramming and systemic T-cell activation dampening.