Neocortical ectopias in BXSB mice: effects upon reference and working memory systems

BXSB mice have an approximately 40-60% incidence of neocortical ectopias in layer I of the prefrontal/motor cortex. Prior studies have found major behavioral differences between those with ectopias and their non-ectopic littermates. Some of these findings indicate that the two groups differ with respect to spatial reference and working memory. The purpose of this study was to measure reference and working memory in the same animals to test the hypothesis that the ectopics would have better reference memory but less effective working memory. The Lashley III maze has cul-de-sacs which must be eliminated, and T-choices where the animal has to decide whether to go left or right. Ectopic and non-ectopic mice were equally able to learn the maze and did not differ on cul-entry or T-choice errors. Then the maze was inverted and the animals were retested. Turning the maze upside down did not change the relative status of the blind alleys. Therefore, the reference memory knowledge from the prior week's training could be used to avoid entering the culs. However, inverting the maze caused a left-right mirror image reversal of the T-choices. Therefore, prior reference memory information would interfere with learning the new path through the maze, whereas working memory would enable the mouse to eliminate T-choice errors. Ectopic mice made less cul-entry errors and more T-choice errors than their non-ectopic littermates, as predicted.     

Nipple attachment in rats during the first 24 hours of life.

Investigated nipple attachment in 49 Purdue-Wistar laboratory rat pups during the 1st 24 hrs of life and identified determinants that influenced its expression. In Exp I, nipple attachment was shown to be age dependent: 1-hr-old pups exhibited a low percentage of attachment, and 12- and 24-hr-old pups exhibited high percentages when tested against an anesthetized lactator. Exps II–VI established the time course of nipple attachment as age related. Exps VII–X explored the behavioral mechanism responsible for the increase in attachment from Hr 1 to Hr 12. They showed that nipple experience, independent of milk acquisition, was the responsible factor for the increase. The findings suggest that learning is employed as a behavioral strategy by the newborn, and they reemphasize the value of using species-relevant measures in the assessment of behavioral development. (35 ref) (PsycINFO Database Record (c) 2010 APA, all rights reserved)     

Handling in infancy, taste aversion, and brain laterality in rats

Rats were handled for the first 20 days of life or were not disturbed. When adults, they were trained to approach and drink from a bottle containing sweetened milk and were then given an injection of lithium chloride to induce a taste aversion conditioned emotional response. Others were injected with physiological saline. Rats within each of the treatment groups were then randomly assigned to 4 surgical procedures: removal of the right or left neocortex; sham surgery; or no surgery. Postoperatively, they were tested for retention of taste aversion by presenting the sweetened milk and recording the amount consumed. The initial consummatory behavior was very low (showing retention of the aversion) and increased over time. There were no differences in the reacquisition curves of the non-handled groups which had received lithium chloride. The curves of the handled groups did differ: thos with an intact right hemisphere (left neocortical lesion) had the lowest asymptote, followed by the group with an intact left brain, while those with intact whole brains consumed the greatest amount of milk. In the groups given an injection of physiological saline, those with a left hemisphere lesion consumed less milk than the other groups, regardless of their early handling experience. The data show: (1) that the rat's brain is lateralized, with the right hemisphere being preferentially involved in conditioned emotional behavior; and (2) that handling in infancy makes the left hemisphere less suceptible to conditioned fear.     

Distinguishing Whether Dopamine Regulates Liking, Wanting, and/or Learning About Rewards.

To determine whether dopamine regulates liking, wanting, and/or learning about rewards during goal-directed behavior, the authors tested genetically engineered dopamine-deficient (DD) mice for acquisition of an appetitive T-maze task with and without endogenous dopamine signaling. Experiment 1 established that DD mice treated with L-dihydroxyphenylalanine (L-dopa [LD]) perform similarly to controls on a T-maze task designed to measure liking, wanting, and learning about rewards. Experiment 2, which tested saline-, caffeine-, and LD-treated DD mice on the T maze, separated performance factors from cognitive processes and revealed that dopamine is not necessary for mice to like or learn about rewards but is necessary for mice to seek (want) rewards during goal-directed behavior. (PsycINFO Database Record (c) 2010 APA, all rights reserved)     

The use of the split-litter technique in psychological research.

The assumption that all genetic factors are held constant in the use of the split-litter technique is examined. On the basis of the available research evidence it can be concluded that within any one litter there will probably be some genetic variation. A decision as to the advisability of using the technique must depend upon whether the control gained over some genetic and environmental factors within the litter compensate for the variability introduced by the unknown genetic factors and other variable factors present within the litter environment. (PsycINFO Database Record (c) 2010 APA, all rights reserved)     

Circulating methemoglobin and nitrite/nitrate concentrations as indicators of nitric oxide overproduction in critically ill children with septic shock

OBJECTIVES: To examine the relationship between circulating methemoglobin and nitrite/nitrate concentrations and to compare these markers of nitric oxide overproduction with clinical variables in children diagnosed with septic shock. DESIGN: Prospective, controlled, clinical study. SETTING: Pediatric intensive care unit and outpatient clinic in a children's hospital. PATIENTS: Twenty-two children diagnosed with septic shock and ten age-matched healthy control patients. INTERVENTIONS: Patients diagnosed with septic shock had blood specimens taken on study entry and every 6 hrs for 72 hrs for methemoglobin and nitrite/nitrate determinations. Single blood specimens were obtained from controls. MEASUREMENTS AND MAIN RESULTS: Circulating methemoglobin and nitrite/nitrate concentrations were significantly higher in children diagnosed with septic shock in comparison with healthy control children (p = .01 and .05, respectively). Peak nitrite/nitrate concentrations correlated with serum creatinine (r2 = .19; p = .04) and were inversely correlated with arterial pH (r2 = .28; p = .01) and urine output (r2 = .21; p = .03) when analyzed by log-linear regression. There were no significant relationships between methemoglobin and nitrite/nitrate or between methemoglobin and any other clinical variable. CONCLUSIONS: Circulating methemoglobin and nitrite/nitrate concentrations are increased in children diagnosed with septic shock. Plasma nitrite/nitrate values correlate with selected clinical variables in these children. Circulating methemoglobin measurements are not superior to plasma nitrite/nitrate concentrations as an indicator of endogenous overproduction of nitric oxide in children diagnosed with septic shock. A need remains to develop markers of endogenous nitric oxide activity that have greater accuracy and reliability.     

Effects of subtle mid-frequency auditory dysfunction upon speech discrimination in noise

Earlier studies have indicated mid-frequency auditory dysfunction and depressed ability to discriminate speech in noise among noise-exposed listeners with high-frequency hearing loss. The present study was designed to determine whether mid-frequency dysfunction contributed to the depressed speech discrimination performance. Normal listeners, and noise-exposed and older listeners with high-frequency hearing loss listened to word lists presented in competing 'cocktail party' noise under unfiltered and low-pass filter conditions. In the low-pass filter condition the performance of the noise-exposed listeners was superior to that normal listeners, indicating that mid-frequency auditory dysfunction on the part of noise-exposed listeners does not contribute to their difficulties discriminating unfiltered speech in noise. The performance of the older listeners was below that of the two other groups in both filtered and unfiltered conditions, indicating greater difficulty discriminating speech than would be predicted only on the basis of high-frequency hearing loss.     

Ordered and chaotic electrical solitons: communication perspectives

While the use of sinusoidal electromagnetic waves as information carriers is taken as one of the principal axioms of today's wireless system design, certain nonsinusoidal waves may further enrich the scope and capacity of modern wireless engineering. Two notable nonsinusoid examples are impulses and chaotic signals. The short temporal width of impulses has enabled applications such as ranging radars and ultra wideband (UWB). The complex nature of chaotic signals offers a new means of encrypted communication. Here we review a new circuit paradigm, the electrical soliton oscillator, which can self-generate both impulse and chaotic signals of very large bandwidth by leveraging the singular dynamics of a nonlinear wave known as the electrical soliton. By combining a nonlinear transmission line with a unique amplifier that can "tame" the inherently unruly dynamics of solitons, the oscillator self-generates a stable, periodic train of short impulses. If the taming functions of the amplifier are turned off, the circuit self-generates chaotic signals by positively exploiting solitons' unruly nature. While still in its early stages, this soliton circuit may one day serve as the heartbeat of both impulse and chaotic wireless systems     

The complexity of the satisfiability problem for Krom formulas

We study the computational complexity of the satisfiability problem for Krom (CNF-2) formulas. Upper and lower bounds are obtained for several decidable classes of formulas determined by quantifier prefix and degree of predicate letters. For example, we show that determining satisfiability of Krom formulas with quantifier prefix of the form ……… is complete for deterministic exponential time, but that if the number of universal quantifiers is bounded then polynomial time suffices.