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Abhijit Gadhe Sandeep Mudliar Ramavatar Pandey S Elumalai Dewanand Satpute 《Journal of chemical technology and biotechnology (Oxford, Oxfordshire : 1986)》2011,86(12):1535-1541
BACKGROUND: Vanillic acid is a flavoring agent and also serves as precursor for vanillin production. Culture medium and fermentation condition for the single step production of vanillic acid from Phanerochaete chrysosporium using lignocellulosic waste as a substrate under solid state fermentation (SSF) were optimized using response surface methodology. RESULTS: The process parameters were chosen by borrowing methodology, and L‐asparagine, pH and moisture content of the solid medium during SSF were identified as the most significant variables. The optimum value of selected variable and their mutual interactions were determined by response surface methodology. The result demonstrated that a yield of 73.58 mg vanillic acid g?1 substate was predicted under optimum conditions (L‐asparagine 5.98 mmol L?1 (2.37 mg g?1 groundnut shell), pH of solid medium 4.51 and moisture content 74.83%). The predicted response was experimentally validated and resulted in a maximum vanillic acid yield of 73.69 mg g?1 after 8 days of SSF. CONCLUSION: The optimization of fermentation variables resulted in a maximum 10‐fold increase in vanillic acid yield compared with that observed under sub‐optimal conditions (from 7.2 mg g?1 to 73.69 mg g?1). Copyright © 2011 Society of Chemical Industry 相似文献
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Saumita Banerjee Ramkrishna Sen R.A. Pandey Tapan Chakrabarti Dewanand Satpute Balendu Shekher Giri Sandeep Mudliar 《Biomass & bioenergy》2009,33(12):1680-1686
The pretreatment of rice husk by the wet air oxidation (WAO) technique was investigated by means of a statistically designed set of experiments. Reaction temperature, air pressure, and reaction time were the process parameters considered. WAO pretreatment of rice husk increased the cellulose content of the solid fraction by virtue of lignin removal and hemicellulose solubilization. The cellulose recovery was around 92%, while lignin recovery was in the tune of 8–20%, indicating oxidation of a bulk quantity of lignin. The liquid fraction was found to be rich in hexose and pentose sugars, which could be directly utilized as substrate for ethanol fermentation. The WAO process was optimized by multi-objective numerical optimization with the help of MINITAB 14 suite of statistical software, and an optimum WAO condition of 185 °C, 0.5 MPa, and 15 min was predicted and experimentally validated to give 67% (w/w) cellulose content in the solid fraction, along with 89% lignin removal, and 70% hemicellulose solubilization; 13.1 gl?1 glucose and 3.4 gl?1 xylose were detected in the liquid fraction. The high cellulose content and negligible residual lignin in the solid fraction would greatly facilitate subsequent enzymatic hydrolysis, and result in improved ethanol yields from rice husk. 相似文献
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Anshari Md Anwar Ali Mishra Rajnish Imam Murshid Pandit Dewanand Jha Kishor Kumar Kesharwani Rahul Kar Manoranjan Chinthapenta Viswanath 《Metallurgical and Materials Transactions A》2023,54(3):869-895
Metallurgical and Materials Transactions A - The periodic structural characteristics of the grains in the overlapping region of the WAAM deposited additive bead translate into spatially dependent... 相似文献
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