Activity of two lignin biosynthesis enzymes during development of a maize internode

To ascertain the temporal relationships of phcnylpropanoid and lignin pathway enzymes, phenylalanine ammonia-lyase (PAL) and cinnamyl alcohol- NADPH dehydrogenase(CAD), with lignin concentration, PAL and CAD activities and lignin concentrations were assessed during progressive development of the 10th internode in maize (Zea mays L). Enzyme activities were quantified and lignin concentrations were determined by the detergent system of fiber fractiona- tion from lower, middle, and upper sections of the elongating internode harvested 3, 5, 7, 9, 11, 13, and 15 days after the internode had reached 10 mm in length. Over 15 days, a coordinated, sequential, and basipetal pattern of enzyme activities and lignin accumulation evolved through the internode, spanning the stages of rapid elongation, cellular differentiation, and secondary cell wall formation. PAL activity was initiated first, rising to peak activity in elongating tissue, and falling basipetally in tissues as they matured. CAD activity rose in fully elongated, maturing internode tissue where PAL activity was waning following its peak activity. Lignin accumulated in tissues with high CAD activity. CAD activity and lignin deposition patterns were similar: simultaneously increasing in activity and deposition over time while activity and deposition also shifting basipetally through the internode, keeping pace with secondary cell wall formation. Lignin concentration correlated significantly with CAD, but not with PAL.     

Laser Induced Liquid Phase Reaction Synthesis Assisted Joining of Metal Matrix Composites

A Laser Induced Liquid Phase Reaction Synthesis Assisted Joining technique is employed for SiC-particulate/Al-alloy composite to produce joints. Joints in SiC/Al-alloy composite are produced by synthesis of suitable material product as a result of interaction between composite and Ti (or Ti-alloy) reactive filler material induced by laser energy in the joint region. Such reaction product minimizes or eliminates the formation of deleterious aluminum carbide phase in the joint region depending upon the type and nature of die interfacial reactive filler material and also the laser processing parameters. A laser beam is utilized to both synthesize the interfacial reactant mixture and to heat the base material adjacent to the joint region to minimize the thermal stresses. The technique along with suitable filler material, further can be extended to a variety of metal matrix composite systems including combinations of Gr/Al, B/Al, B₄C/Mg, Steel/Al, W/Al, Al₂ O₃ and Gr/Cu which are excellent for use in various automotive, aerospace and electronic applications. Preliminary observations describing the proof of concept of laser induced reaction joining of metal matrix composites are reported     

Drug treatment evaluation research in the United States.

Describes the Drug Abuse Reporting Program, the 1st national evaluation study of community-based treatment programs, implemented in the early 1970s. It evolved into a 20-yr longitudinal research program that included during-treatment assessments of client performance, evaluations of treatment effectiveness based on outcomes in the years immediately following treatment, and long-term studies of addiction careers. The Treatment Outcome Prospective Study began in the early 1980s and is now being followed by the Drug Abuse Treatment Outcome Study in the 1990s. These multisite and multimodality studies have helped establish broad-based evidence for treatment efficacy and directions for new studies in the US. The AIDS crisis has had the effect of sharply increasing federal research funds, including grants allocated to enhance and evaluate psychosocial intervention programs for drug users. (PsycINFO Database Record (c) 2010 APA, all rights reserved)     

Effectiveness of participatory ergonomic interventions on health outcomes: a systematic review

The objective of this study was to conduct a systematic review of the literature on the effectiveness of participatory ergonomic (PE) interventions for improving workers' health. The search strategy targeted six electronic databases and identified 442 potential articles. Each article was examined by pairs of reviewers for relevance (assessed a participative ergonomic workplace intervention, with at least one health outcome, published in English in peer reviewed literature). Twenty-three articles met relevance criteria and were then appraised for methodological strength. Using a best evidence synthesis approach, 12 studies that were rated as 'medium' or higher provided partial to moderate evidence that PE interventions have a positive impact on: musculoskeletal symptoms, reducing injuries and workers' compensation claims, and a reduction in lost days from work or sickness absence. However, the magnitude of the effect requires more precise definition.     

Comparison of the relative recovery of polyphenolics in two fruit extracts from a model of degradation during digestion and metabolism

To simulate the effects of digestion and metabolism on the survival of different polyphenolic compounds, extracts of blueberry and apple were deglycosylated by acid hydrolysis, followed by enzymic glucuronidation under neutral conditions, yielding approximately 5% overall recovery of polyphenolics. The major polyphenolics before and after the treatment were compared, to estimate which species are likely to be present in the intestinal lumen, undegraded and available for absorption, after consumption of the fruit. Whereas blueberry extract consisted predominantly of anthocyanins, epicatechin and caffeoyl quinate esters, the major components of the treated extract were quercetin glucuronides and (unglucuronidated) caffeoyl quinates, with only traces of anthocyanidin derivatives. In apple extract, compositional changes were less marked, but caffeoyl quinates, procyanidins and quercetin were enriched at the expense of caffeic acid, epicatechin and catechin. Hydrophobic compounds like phloretin and quercetin were extensively glucuronidated, whereas caffeic acid and caffeoyl quinate were not. These results suggest that the major polyphenolic components of a fruit are not necessarily the most important contributors to any health benefits because the polyphenolic composition in the intestinal lumen and consequently, in the circulation, may be considerably different.     

Inhaled PYY(3–36) dry-powder formulation for appetite suppression

Objective: Peptide YY3–36 [PYY(3–36)] has shown efficacy in appetite suppression when dosed by injection modalities (intraperitoneal (IP)/subcutaneous). Transitioning to needle-free delivery, towards inhalation, often utilizes systemic pharmacokinetics as a key endpoint to compare different delivery methods and doses. Systemic pharmacokinetics were evaluated for PYY3–36 when delivered by IP, subcutaneous, and inhalation, the systemic pharmacokinetics were then used to select doses in an appetite suppression pharmacodynamic study.

Methods: Dry-powder formulations were manufactured by spray drying and delivered to mice via nose only inhalation. The systemic plasma, lung tissue, and bronchoalveolar lavage fluid pharmacokinetics of different inhalation doses of PYY(3–36) were compared to IP and subcutaneous efficacious doses. Based on these pharmacokinetic data, inhalation doses of 70:30 PYY(3–36):Dextran T10 were evaluated in a mouse model of appetite suppression and compared to IP and subcutaneous data.

Results: Inhalation pharmacokinetic studies showed that plasma exposure was similar for a 2?×?higher inhalation dose when compared to subcutaneous and IP delivery. Inhalation doses of 0.22 and 0.65?mg/kg were for efficacy studies. The results showed a dose-dependent (not dose proportional) decrease in food consumption over 4?h, which is similar to IP and subcutaneous delivery routes.

Conclusions: The pharmacokinetic and pharmacodynamics results substantiate the ability of pharmacokinetic data to inform pharmacodynamics dose selection for inhalation delivery of the peptide PYY(3–36). Additionally, engineered PYY(3–36):Dextran T10 particles delivered to the respiratory tract show promise as a non-invasive therapeutic for appetite suppression.     

Relation of Spin Susceptibility and a.c. Conductivity in Nearly Antiferromagnetic Fermi Liquid

Coupling of the charge carriers through the magnetic spin susceptibility which is strongly peaked at (, ) in the two dimensional CuO ₂ Brillouin zone leads to strong momentum anisotropies and energy dependencies in the inelastic optical scattering rates. The a.c. conductivity is isotropic and can be used to extract an effective, isotropic spin-charge carrier spectral density which reproduces almost exactly the optical scattering rates. The technique is then applied to data in superconducting, optimally doped YBCO and used to measure the charge carrier coupling to the 41 meV peak observed in spin polarized neutron scattering.     

Laser surface modification of zinc-base composites

The unique, ultrafine microstructure that evolves during laser surface modification of a zinc-base metal-matrix composite (MMC) directly affects surface properties such as corrosion and wear. Preliminary studies indicate that over the experimental range of conditions, the corrosion rate and the wear rate of the laser surface-treated MMC are lower than of that the untreated MMC. Further, x-ray diffractometry analysis of the laser surface-treated sample shows that unconventional, nonequilibrium phases are created in the process.     

An assessment of comorbid psychological problems in a residential criminal justice drug treatment program.

161 probationers remanded to 4 mo of mandatory treatment at a criminal justice substance abuse treatment facility were assessed for evidence of psychopathology by means of the Millon Clinical Multiaxial Inventory-II and other psychosocial functioning scales. Overall, 80% of the sample was classified as having psychological problems, 72% had significant drug abuse problems, and 58% had concurrent psychopathology and drug abuse problems (i.e., comorbidity). Comparisons between psychopathology-only, drug problem-only, and comorbid groups indicated that probationers with both drug use and psychological problems engaged in more preadmission illegal activity, had more social impairments, and indicated higher motivation for treatment. (PsycINFO Database Record (c) 2010 APA, all rights reserved)     

Treatment retention and follow-up outcomes in the Drug Abuse Treatment Outcome Study (DATOS).

Clients in the national Drug Abuse Treatment Outcome Study reported significant overall improvements in drug use and related measures during a 12-month follow-up period. A quasi-experimental design was used to examine the relationship of treatment duration with outcomes in each of the 3 major modalities represented. Client subsamples with longer retention in long-term residential programs and in outpatient methadone treatment had significantly better outcomes than those with shorter lengths of stay (results were inconclusive for outpatient drug-free programs because of sample limitations). This study used several methodological enhancements and showed general continuity of findings on retention effects from previous national evaluations of treatment effectiveness. It supports the need for more careful study of treatment process in relation to outcomes. (PsycINFO Database Record (c) 2010 APA, all rights reserved)