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排序方式: 共有270条查询结果,搜索用时 31 毫秒
1.
MH Hennington AM D'Armini JJ Lemasters TM Egan 《Canadian Metallurgical Quarterly》1996,61(7):1009-1014
In an effort to increase the donor pool for lung transplantation (LTX), we have demonstrated the feasibility of LTX from circulation-arrested cadavers in a canine LTX model. We hypothesized that ventilation of the cadaver lung with alveolar gas (20% O2, 5% CO2, balance N2) (AG) would be superior to ventilation with 100% oxygen (O2) after circulatory arrest of the donor. Twelve mongrel dogs were intubated, heparinized and euthanized by pentothal injection and ventilated with AG (n=6) or O2 (n=6). Four hours later, donor animals underwent sternotomy, and the lungs were flushed with cold modified Euro-Collins solution, harvested, and stored inflated in ice slush. Left lung allotransplantation was performed, and recipients were made dependent o n the transplanted lung by occlusion of the contralateral bronchus and pulmonary artery. Recipient animals were ventilated with an FiO2 of 0.4 and followed for 8 hr. Total ischemic time was 7.9 hr for both groups. Pulmonary edema developed in all recipients of AG lungs; one recipient survived the 8-hr observation period with poor oxygenation. In contrast, three of six recipients of O2-ventilated lungs survived for 8-hr with excellent gas exchange. Specimens of donor lungs before and after transplant were evaluated histologically utilizing trypan blue exclusion as an indicator of cell viability. At the time of organ retrieval 4 hr after death, 6% of cells were nonviable in the O2-ventilated cadaver lungs. Circulation-arrested cadaver lungs ventilated with 100% O2 prior to organ retrieval have superior pulmonary function after transplant compared with lungs ventilated with AG. Ventilation of cadaver lungs with AG induces pulmonary injury in this model. retrieval of donor lungs from circulation-arrested cadavers has potential for increasing the pulmonary donor pool. 相似文献
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KL Gatford TP Fletcher IJ Clarke PC Owens KJ Quinn PE Walton PA Grant BJ Hosking AR Egan EN Ponnampalam 《Canadian Metallurgical Quarterly》1996,74(6):1314-1325
The effects of sex and age on patterns of circulating somatotropin (ST) concentration and plasma IGF-I, IGF-II, insulin, and IGF binding protein-3 (IGFBP-3) were studied in ram, wether, and ewe lambs (n = 7 or 8) sampled at mean ages of 81 (I1) and 158 d (I2). Between 81 and 158 d of age, rams grew more rapidly than wethers (P < .01), and wethers grew more rapidly than ewes (P < .01). The sex differences in growth were reflected in empty body weight at slaughter: rams > wethers > ewes (P < .05). Mean plasma ST concentrations, ST pulse amplitude, and integrated plasma ST concentrations were greater (P < .05) in rams than in ewes at I1 and I2. Characteristics of the ST plasma profile in wethers were generally intermediate between those of rams and ewes. The interpulse interval was greater in ewes than in wethers at I2. The IGF-I and IGFBP-3 concentrations were greater in rams than in ewes at both sampling times. Plasma IGF-II was greater in ewes than in rams at I2. Mean plasma ST was approximately two thirds less at I2 than at I1 regardless of sex. Mean plasma ST and IGF-I at both ages were positively correlated with growth. Mean plasma ST at I2 was negatively correlated with fatness at slaughter. Sex and age significantly affected patterns of circulating ST and concentrations of IGF-I and IGFBP-3 in prepubertal growing lambs, under conditions for which growth rates and composition were also sexually dimorphic. 相似文献
4.
P Fiset HL Lemmens TD Egan SL Shafer DR Stanski TE Egan 《Canadian Metallurgical Quarterly》1995,58(5):567-582
The purpose of this study was to model pharmacodynamically the reversal of midazolam sedation with flumazenil. Ten human volunteers underwent four different sessions. In session 1, individual midazolam pharmacokinetics and electroencephalographic pharmacodynamics were determined. In sessions 2 and 3, a computer-controlled infusion of midazolam with individual volunteer pharmacokinetic data was administered, targeting a plasma concentration corresponding to a light or deep level of sedation (20% or 80% of the maximal midazolam electroencephalographic effect) for a period of 210 minutes. After obtaining a stable electroencephalographic effect and constant midazolam plasma concentrations, a zero-order infusion of flumazenil was started until complete reversal of midazolam electroencephalographic effect was obtained. The flumazenil infusion was then stopped and the volunteer was allowed to resedate because of the constant midazolam drug effect. The electroencephalographic response was measured during a 180-minute period and analyzed by aperiodic analysis and fast-Fourier transforms. In session 4, a midazolam plasma concentration corresponding to a deep level of sedation was targeted for 210 minutes to examine for the possible development of acute tolerance. No flumazenil was given in session 4. For a light sedation level, with a mean midazolam plasma concentration of 160 +/- 64 ng/ml, the mean half-life of the equilibration rate constant of flumazenil reversal is 5.0 +/- 2.5 minutes, and the mean effect site concentration causing 50% of Emax is 13.7 +/- 5.8 ng/ml. For a deep level of sedation, with a mean midazolam plasma concentration of 551 +/- 196 ng/ml, the mean half-life of the equilibration rate constant is 3.9 +/- 1.5 minutes, and the mean effect site concentration causing 50% of Emax is 20.6 +/- 6.8 ng/ml. This study provides an estimate of the magnitude of the blood/central nervous system equilibration delay for flumazenil antagonism of midazolam sedation and further defines the usefulness of the electroencephalogram as a measure of midazolam pharmacodynamic effect. 相似文献
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The fracture of polyethylene has been studied extensively using conventional testing geometries such as three-point bending (TPB) and single-edge notch tension (SENT). These geometries are of limited utility for studying crack growth, because the crack speed is constantly changing and the crack front is in the centre of the specimen. Double torsion (DT) is a fracture geometry that suffers neither of these disadvantages, yet has only received limited attention in the literature. Its use has been limited to highly brittle materials such as glass, ceramics, thermosetting plastics and PMMA. In contrast to these materials, high-density polyethylene (HDPE) is an inherently ductile polymer. Before the advantages of DT can be exploited for testing HDPE, it is first necessary to demonstrate the validity of DT fracture measurements performed on such a ductile material. In this paper it is shown that at moderate rates of loading and at temperatures below 0C, valid double torsion fracture results can be obtained for an ethylene 1-butene copolymer. A novel technique for specimen preparation and a simple method for accurately monitoring crack growth are also described. 相似文献
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Inside Cover: Site‐Specific Immobilization of the Peptidoglycan Synthase PBP1B on a Surface Plasmon Resonance Chip Surface (ChemBioChem 23/2016) 下载免费PDF全文
8.
Ugarov MV Egan T Khabashesku DV Schultz JA Peng H Khabashesku VN Furutani H Prather KS Wang HW Jackson SN Woods AS 《Analytical chemistry》2004,76(22):6734-6742
When used in small molar ratios of matrix to analyte, derivatized fullerenes and single wall nanotubes are shown to be efficient matrices for matrix-assisted laser desorption/ionization (MALDI) mass spectrometry. The mixing of an acidic functionalized fullerene with a solution of bioanalyte, depositing a dried droplet, and irradiating with a pulsed nitrogen laser yields protonated or cationized molecular ions. Derivatized fullerenes could offer several advantages over conventional MALDI matrices: a high analyte ionization efficiency, a small molar ratios (less than 1) of matrix/analyte, and a broader optical absorption spectrum, which should obviate specific wavelength lasers for MALDI acquisitions. The major disadvantage to the use of fullerenes is the isobaric interference between matrix and analyte ions; however, it is overcome by using MALDI-ion mobility time-of-flight (IM-oTOF) mass spectrometry to preseparate carbon cluster ions from bioanalyte ions prior to TOF mass analysis. However, an alternative to the dried droplet preparation of fullerene MALDI samples is the aerosolization of matrix-analyte solutions (or slurries) followed by impacting the aerosol onto a stainless surface. We also demonstrate that the fullerene matrices can be used to acquire spectra from rat brain tissue. 相似文献
9.
Michael J. Fansler Janet L. Egan Haluk Bekiroglu 《Computers & Industrial Engineering》1978,2(1):27-30
Operation of the remote batch terminals connected to an IBM 370/158 computer system at a large mid-western firm is simulated using GPSS language. The performance of the computer system was simulated to determine the impact of changes to be imposed on the system. The model parameters of remote printer speed and core storage capacity were altered during the course of the simulation. These variables were selected for their flexibility in relationship to the total network and the relative ease of implementing such changes. It is found that implementing higher speed printers and increased computer core storage capacity can decrease printer and core storage utilizations and the average service rate drastically. 相似文献
10.
This paper addresses issues that relate to downscaling the height of centrifugal fans for application in low profile technologies, such as the cooling of portable power electronics. The parameters studied include flow rate, pressure rise, and power consumption characteristics. The former two of these are measured using a fan characterization rig and the latter by directly measuring the power supplied to the fan. These are studied for fan diameters ranging from 15 to 30 mm with numerous profile heights between 0.3 mm and 15 mm. It is found that all of the phenomena encountered are best described in terms of fan aspect ratio. The results show that the conventional scaling laws cannot be accurately applied when blade profile alone is scaled. Indeed, the only parameter reasonably well predicted was the pressure rise attainable, but that was only accurate for fan aspect ratios greater than 0.17. Below this, the pressure rise generated reduces logarithmically toward zero. The study also reveals that no advantage is gained by using fans of aspect ratio greater than 0.3, as the maximum flow rate attainable decreases slightly above this. Overall, the scaling phenomena reported herein provide invaluable information for the future design of efficient low-profile cooling solutions that are to incorporate such fans. 相似文献