An acidic ionic liquid-conventional alkali-catalyzed biodiesel production process

A study was undertaken to prepare biodiesel via two-step process using ionic liquid as first step catalyst due to the unsuitability of using the straight alkaline-catalyzed transesterification of high FFA presented in crude palm oil (CPO). In the first step, esterification of the FFA presented in the CPO was carried out using butylimidazolium hydrogen sulfate (BIMHSO₄), in which the acid value was reduced from 6.93 to 1.02mg KOH/g and then, KOH-catalyzed transesterification was applied. The conversion rate of FFA attained 85.3% when 4.8 wt% of BIMHSO₄ was applied to the reaction system containing methanol to CPO ratio of 12: 1 reacted at 170 °C for 150min. The final yield in 97.3% revealed that the process proposed in this study could lead to an excellent biodiesel meeting the ASTM requirements. Furthermore, this new two-step catalysis process could solve the old conventional catalysis process drawbacks.     

POLYBiNN: Binary Inference Engine for Neural Networks using Decision Trees

Journal of Signal Processing Systems - Convolutional Neural Networks (CNNs) and Deep Neural Networks (DNNs) have gained significant popularity in several classification and regression applications....     

Fibril density reduction in keratoconic corneas

This study aims to estimate the reduction in collagen fibril density within the central 6 mm radius of keratoconic corneas through the processing of microstructure and videokeratography data. Collagen fibril distribution maps and topography maps were obtained for seven keratoconic and six healthy corneas, and topographic features were assessed to detect and calculate the area of the cone in each keratoconic eye. The reduction in collagen fibril density within the cone area was estimated with reference to the same region in the characteristic collagen fibril maps of healthy corneas. Together with minimum thickness and mean central corneal refractive power, the cone area was correlated with the reduction in the cone collagen fibrils. For the corneas considered, the mean area of keratoconic cones was 3.30 ± 1.90 mm². Compared with healthy corneas, fibril density in the cones of keratoconic corneas was lower by as much as 35%, and the mean reduction was 17 ± 10%. A linear approximation was developed to relate the magnitude of reduction to the refractive power, minimum corneal thickness and cone area (R² = 0.95, p < 0.001). Outside the cone area, there was no significant difference between fibril arrangement in healthy and keratoconic corneas. The presented method can predict the mean fibril density in the keratoconic eye''s cone area. The technique can be applied in microstructure-based finite-element models of the eye to regulate its stiffness level and the stiffness distribution within the areas affected by keratoconus.     

Towards Quantitative and Standardized Serological and Neutralization Assays for COVID-19

Quantitative and robust serology assays are critical measurements underpinning global COVID-19 response to diagnostic, surveillance, and vaccine development. Here, we report a proof-of-concept approach for the development of quantitative, multiplexed flow cytometry-based serological and neutralization assays. The serology assays test the IgG and IgM against both the full-length spike antigens and the receptor binding domain (RBD) of the spike antigen. Benchmarking against an RBD-specific SARS-CoV IgG reference standard, the anti-SARS-CoV-2 RBD antibody titer was quantified in the range of 37.6 µg/mL to 31.0 ng/mL. The quantitative assays are highly specific with no correlative cross-reactivity with the spike proteins of MERS, SARS1, OC43 and HKU1 viruses. We further demonstrated good correlation between anti-RBD antibody titers and neutralizing antibody titers. The suite of serology and neutralization assays help to improve measurement confidence and are complementary and foundational for clinical and epidemiologic studies.     

Electrofusion of zona-free mouse embryonic cells in electrolytes and their development in vitro

The influence of increasing the physical electrofusion parameters, direct current (DC) pulse strength, pulse duration, pulse number, alternating current (AC) voltage and alignment time, in electrolytes on the rates of fusion, degeneration and development of zona-free mouse 2-cell embryos were examined. Furthermore, the effects of physiological saline and mannitol as fusion media and various mouse strains were also evaluated. Dulbecco's phosphate-buffered saline (PBS) supplemented with 10% fetal calf serum was used as the main fusion solution. A significant increase in the rate of fusion (P < 0.05) was obtained by increasing pulse strength from 30 to 300 V/mm. The embryos fused at the pulse strengths of 30 to 70 V/mm had significantly higher development rates to blastocysts compared with those fused at 100 to 300 V/mm (P < 0.05). There were no significant differences in the rates of fusion, degeneration and development to blastocysts when the pulse duration was increased from 30 to 90 microseconds. Although fusion rates were increased (P < 0.05) by increasing the pulse number up to 4, a significant decrease (P < 0.05) in development to blastocysts was observed when the pulse number was 5. Application of AC voltage prior to the DC pulse tended to increase the fusion rate (89.2-93.8%), compared with fusion with the DC pulse only (75.0%). Prolongation of alignment time from 5 to 15 sec had no effect on the fusion rate. Under the optimum conditions (2 pulses of DC of 70 V/mm, 70 microseconds pulse duration and AC of 5 V/mm for 5 sec), no significant difference was obtained in the fusion and development rates in different mouse strains, nor were fusion and development rates significantly different among PBS, physiological saline and mannitol solutions (P > 0.05).     

A multi-layer perceptron neural network model for predicting the hydrate equilibrium conditions in multi-component hydrocarbon systems

Neural Computing and Applications - This work presents a model based on multilayer perceptron neural network (MLPNN) for the prediction of hydrate equilibrium conditions in hydrocarbon systems. The...     

Covid-19’s Pandemic Relationship to Saudi Arabia’s Weather Using Statistical Analysis and GIS

The eruption of the novel Covid-19 has changed the socio-economic conditions of the world. The escalating number of infections and deaths seriously threatened human health when it became a pandemic from an epidemic. It developed into an alarming situation when the World Health Organization (WHO) declared a health emergency in MARCH 2020. The geographic settings and weather conditions are systematically linked to the spread of the epidemic. The concentration of population and weather attributes remains vital to study a pandemic such as Covid-19. The current work aims to explore the relationship of the population, weather conditions (humidity and temperature) with the reported novel Covid-19 cases in the Kingdom of Saudi Arabia (KSA). For the study, the data for the reported Covid-19 cases was secured from 11 March 2020, to 21 July 2020 (132 days) from the 13 provinces of KSA. The Governorate level data was used to estimate the population data. A Geographic information system (GIS) analysis was utilised to visualise the relationship. The results suggested that a significant correlation existed between the population and Covid-19 cases. For the weather conditions, the data for the 13 provinces of KSA for the same period was utilised to estimate the relationship between the weather conditions and Covid-19 cases. Spearman’s rank correlation results confirmed that the humidity was significantly linked with the reported cases of Covid-19 in Makkah, Aseer, Najran, and Al Baha provinces. The temperature had a significant relation with the reported Covid-19 cases in Al-Riyad, Makkah, Al-Madinah, Aseer, Najran, and Al-Baha. The inconsistency of the results highlighted the variant behavior of Covid-19 in different regions of the KSA. More exploration is required beyond the weather-related variables. Suggestions for future research and policy direction are offered at the end of the study.     

Effects of intercropping, Bradyrhizobium inoculation and chicken manure fertilisation on the chemical composition and physical characteristics of soybean seed

A field experiment was carried out to investigate the effect of two Bradyrhizobium strains (local and imported), chicken manure fertilisation (7 t/ha) and intercropping with sorghum on the chemical composition and physical characteristics of soybean seed. For both monocropping and intercropping systems, moisture content slightly increased for both systems and for all treatments, while ash, fibre and carbohydrate contents fluctuated for both systems and treatments. The protein content of the seeds was significantly (p ? 0.05) increased for all treatments. Tannin content was increased significantly (p ? 0.05) with a concomitant decrease in protein digestibility for both systems and for all treatments. The seed weight (100 seeds), hydration coefficient and cookability were increased for all treatments. Mineral composition of the seeds was increased and the increment varied with different treatments.     

An experimental analysis of a flaring process for tube ends using a novel spinning tool

The flaring of tube ends are typically performed using electromagnetic forming, or punch-and-die method, both of which cost time and effort. In this work, a new method of tube flaring using a metal spinning process is proposed. An experimental study was carried out to flare the end of aluminium tubes. A multifunction tool was designed based on the flaring ratio to widen the tube end. Several flared specimens were conducted at different working conditions. The effects of working conditions such as flaring ratio, axial feed rate (mm/rev), and rotating speed (rpm) on the flaring loads were investigated. An experimental analysis was carried out to illustrate the effect of the working conditions on the characteristics of the formed part. The analysis showed that these working conditions have a clear influence on wall thickness, surface hardness, and flaring loads.