7-(Dialkylamino)coumarin-4-yl]methyl-Caged Compounds as Ultrafast and Effective Long-Wavelength Phototriggers of 8-Bromo-Substituted Cyclic Nucleotides

[7-(Dimethylamino)coumarin-4-yl]methyl (DMACM) and [7-(diethylamino)coumarin-4-yl]methyl (DEACM) esters of 8-bromoadenosine 3',5'-cyclic monophosphate (8-Br-cAMP) and 8-bromoguanosine 3',5'-cyclic monophosphate (8-Br-cGMP) are described as novel caged compounds for 8-bromo-substituted cyclic nucleotides. Synthesis is accomplished by treatment of the free acids of the cyclic nucleotides with the corresponding 7(dialkylamino)-substituted 4(diazomethyl)coumarins. Irradiation of the DMACM- and DEACM-caged cyclic nucleotides with UV light stimulates the release of the cyclic nucleotides within roughly a nanosecond. The new caged compounds are resistant to hydrolysis in aqueous buffers and exhibit long-wavelength absorption properties with maxima at 400 nm, high extinction coefficients, and high quantum yields (0.15-0.31). Their favorable properties render these compounds the most efficient and rapid phototriggers of 8-bromo-substituted cyclic nucleotides known. The usefulness of the compounds for physiological studies under nondamaging light conditions was examined in HEK293 cells expressing the alpha subunit of the cyclic-nucleotide-gated (CNG) channel of cone photoreceptors (CNGA3) and of olfactory neurons (CNGA2) by using confocal laser scanning microscopy and the patch clamp technique.     

The Golden Section in Architectural Theory

The never-ending—but always young—discussion about the Golden Section in architecture never lacks a hint at Luca Pacioli and architectural theory. But what it always lacks is a thorough study of the Golden Section in architectural theory. This paper aims to present this analysis. Marcus Frings traces the Golden Section from the mathematical and rather theoretical character of Pacioli’s concept, the the treatises of Alberti, Serlio, and Palladio, to Adolf Zeising in the nineteenth century. and to theorist Matila Ghyka and the practitioners Ernst Neufert and Le Corbusier in the twentieth. In the latter’s writings and designs the Golden Section seems to play the role of a scholarly element which shows the master’s theoretical erudition, leading to contemporary architects such as Ricardo Bofill.     

The effect of difference oriented communication on the subjective validity of an in-group norm: Doc can treat the group.

The subjective group dynamics model predicts that in-group deviants who violate in-group norms that differentiate between the in-group and the out-group threaten the in-group's public image and its sense of validity. Previous research has shown that, to reduce this threat, group members attempt to symbolically marginalize in-group deviants through negative evaluation. In the current study (N = 107), the effect of another form of symbolic marginalization (difference oriented communication) is investigated. The findings support the subjective group dynamics model by showing that group members whose communications to deviants highlighted differences experienced a subsequent increase in subjective validity of in-group norms. (PsycINFO Database Record (c) 2010 APA, all rights reserved)     

Environmental requirements in thermochemical and biochemical conversion of biomass

Many biological and thermochemical processing options exist for the conversion of biomass to fuels.

Commercially, these options are assessed in terms of fuel product yield and quality. However, attention must also be paid to the environmental aspects of each technology so that any commercial plant can meet the increasingly stringent environmental legislation in the world today.

The environmental aspects of biological conversion (biogasification and bioliquefaction) and thermal conversion (high pressure liquefaction, flash pyrolysis, and gasification) are reviewed. Biological conversion processes are likely to generate waste streams which are more treatable than those from thermal conversion processes but the available data for thermal liquefaction are very limited. Close attention to waste minimisation is recommended and processing options that greatly reduce or eliminate waste streams have been identified. Product upgrading and it's effect on wastewater quality also requires attention. Emphasis in further research studies needs to be placed on providing authentic waste streams for environmental assessment.     

Strategy effects counteract distractor inhibition: Negative priming with constantly absent probe distractors.

It is an accepted, albeit puzzling finding that negative priming (NP) hinges on the presence of distractors in probe displays. In three experiments without probe distractors, the authors yielded evidence that response-biasing processes based on the contingency between prime and probe displays may have caused this finding. It is argued that it is of help in standard NP experiments to process the distractor in the prime display in order to prepare the response to the probe target. When this contingency was removed (Experiments 2 and 3), NP was reliably observed without probe distractors, whereas no NP emerged if the design contained the typical contingency (Experiment 1). For this reason, the data suggest that the absence of NP, which is usually observed under these conditions, may be due to a contingency-based component. (PsycINFO Database Record (c) 2011 APA, all rights reserved)     

A case for inhibition: Visual attention suppresses the processing of irrelevant objects.

The present study investigated the ability to inhibit the processing of an irrelevant visual object while processing a relevant one. Participants were presented with 2 overlapping shapes (e.g., circle and square) in different colors. The task was to name the color of the relevant object designated by shape. Congruent or incongruent color words appeared in the relevant object, in the irrelevant object, or in the background. Stroop effects indicated how strong the respective area of the display was processed. The results of 4 experiments showed that words in the relevant object produced larger Stroop effects than words in the background, indicating amplification of relevant objects. In addition, words in the irrelevant object consistently produced smaller Stroop effects than words in the background, indicating inhibition of irrelevant objects. Control experiments replicated these findings with brief display durations (250 ms) and ruled out perceptual factors as a possible explanation. In summary, results support the notion of an inhibitory mechanism of object-based attention, which can be applied in addition to the amplification of relevant objects. (PsycINFO Database Record (c) 2010 APA, all rights reserved)     

To be or not to be…included in an event file: Integration and retrieval of distractors in stimulus–response episodes is influenced by perceptual grouping.

The binding of stimulus and response features into stimulus–response (S-R) episodes or “event files” is a basic process for the efficient control of behavior. However, relevant information is usually accompanied by information that is irrelevant for the selection of action. Recent studies showed that even irrelevant information is bound into event files. In this study, we investigated the boundary conditions of distractor–response binding and subsequent distractor-based response retrieval processes. In particular, we tested whether the inclusion of distractor information into S-R episodes is modulated by whether the distractor and target stimulus are perceived as belonging to the same object or as belonging to different objects. We argue that distracting information is only bound into S-R episodes if it is perceived as belonging to the same object as the relevant information, whereas no binding occurs when the distracting information is perceived as belonging to a separate object. In 6 experiments, we found evidence for the modulation of distractor–response bindings according to perceptual grouping principles. (PsycINFO Database Record (c) 2011 APA, all rights reserved)