Relationships among lactate concentration, blood flow and histopathologic profiles in rat C6 glioma

Increased capacity for glycolytic metabolism is a well-known characteristic of neoplastic cells. Because lactic acid is the end product of glycolysis, in vivo MRS measurements of tumor lactate concentration ([lac]t) may provide valuable information about tumor metabolism, which will aid the development of therapies and the clinical diagnosis and treatment of tumors. In the present study, several hemodynamic and histologic parameters were evaluated with respect to their influence on [lac]t. Pronounced differences in [lac]t in two distinct populations of tumors suggested a putative perfusion threshold. Above this threshold, [lac]t was independent of hemodynamic and histologic factors including tumor blood flow (measured using MRS and the method of D2O washout), extent of necrosis and inflammatory cell infiltrate. Thus, for most tumors, [lac]t was not determined by any one single factor such as hypoxia, venous clearance, glucose supply, extent of necrosis or degree of inflammatory cell infiltrate. Rather, [lac]t may be equilibrated, at least in part, by an interplay of forces involving hemodynamics and substrate supply. In general, the data are consistent with the hypothesis that elevated lactate in most tumors is related to the high glycolytic activity of adequately perfused, viable neoplastic cells.     

Infant affect and affect regulation during the still-face paradigm with mothers and fathers: the role of infant characteristics and parental sensitivity

This laboratory study examined mothers' and fathers' sensitivity during face-to-face interactions with their infants as well as infants' affective and regulatory responses during mother-infant versus father-infant still face (SF). The degree to which infant gender and temperament as well as parental sensitivity predicted SF responses was also examined. Participants included 94 healthy, primarily White, middle-class 4-month-olds and their parents. Results indicated that mothers and fathers were equally sensitive toward their infants. Infants' affect and regulatory behaviors were also significantly stable across mother- and father-infant SF situations, although several differences in mean levels of regulation emerged. Finally, the extent to which exogenous and endogenous variables predicted infant SF responses differed as a function of which affect or regulatory variable was being examined and with which parent the infant was experiencing SF.     

Characterization of Starch from Maize Endosperm Mutants

Some maize endosperm mutants have branched-chain starch components with long exterior chains which make the blue-value procedure for estimating amylose content questionable. Gel filtration was used to estimate starch composition of normal, high-amylose, waxy and other maize endosperm mutants. Loosely-branched polymers appeared responsible for the apparent amylose content. In some mutants high and intermediate loosely branched polysaccharides were found in addition to amylose. The sweet corn background of some endosperm genotypes influenced the types of polymers produced compared to the dent backgrounds used by previous researchers.     

Lactate turnover in rat glioma measured by in vivo nuclear magnetic resonance spectroscopy

Elevated tissue lactate concentrations typically found in tumors can be measured by in vivo nuclear magnetic resonance (NMR) spectroscopy. In this study, lactate turnover in rat C6 glioma was determined from in vivo 1H NMR measurements of [3-13C]lactate buildup during steady-state hyperglycemia with [1-13C]glucose. With this tumor model, a narrow range of values was observed for the first-order rate constant that describes lactate efflux, k2 = 0.043 +/- 0.007 (n = 12) SD min-1. For individual animals, the standard error in k2 was small (< 18%), which indicated that the NMR data fit the kinetic model well. Lactate measurements before and after infusing [1-13C]glucose showed that the majority of the tumor lactate pool was metabolically active. Signals from 13C-labeled glutamate in tumors were at least 10-fold smaller than the [3-13C]lactate signal, whereas spectra of the contralateral hemispheres revealed the expected labeling of [4-13C]glutamate, as well as [2-13C] and [3-13C]glutamate, which indicates that label cycled through the tricarboxylic acid cycle in the brain tissue. Lack of significant 13C labeling of glutamate was consistent with low respiratory metabolism in this glioma. It is concluded that lactate in rat C6 glioma is actively turning over and that the kinetics of lactate efflux can be quantified noninvasively by 1H NMR detection of 13C label. This noninvasive NMR approach may offer a valuable tool to help evaluate tumor growth and metabolic responsiveness to therapies.     

我国百万千瓦级核岛主设备实现国产化

由中国一重承制的我国首台完全自主化制造的红沿河核电站1号机组核反应堆压力容器,2010年12月19日在一重大连制造基地完工发往辽宁红沿河,经检测其各项技术指标全部满足质量要求。这标志着我国百万千瓦级核岛主设备的制造经过独立研发、自主创新,已完全实现国产化,达到了国际先进水平,具备了为我国核电建设标准化、批量化、规模化发展提供成套装备的能力。     

Low-energy electron-enhanced etching of HgCdTe

Low-energy electron-enhanced etching (LE4) is applied to HgCdTe to eliminate ion-induced surface damage. First, LE4 results for patterned samples are illustrated. The LE4 mechanism is understood from a mechanistic study in terms of three etch variables: direct current (DC) bias, gas composition, and sample temperature. For this paper, the effects of DC bias (electron energy) and gas composition (CH₄ concentration) are summarized qualitatively, followed by quantitative evidence. Etch rate, the amount of polymer, surface stoichiometry, and surface roughness have specific relations with each etch variable under competition between pure LE4 and polymer deposition.     

Experimental study of effects of prior overload on fracture toughness of A533B steel

This paper presents the results of an extensive study carried out to examine the effects of prior overloading over the entire fracture transition regime for 50-mm thick A533B steel. The main variables examined are temperature, crack orientation with respect to the rolling direction, level of prior overload, the initial crack length, and the statistical variation of prior overload effects. It is found that the effect of prior overload on fracture toughness at lower temperatures is dependent on orientation, so that in the L-T orientation for short and medium cracks (0·2 and 0·5 a/W) there is a benefit throughout the transition regime of 50-mm thick A533B steel. In the T-L orientation no benefit is obtained for temperatures greater than the initiation of tearing temperatures. Above these temperatures the prior overload sequence lowers the fracture toughness. For L-T orientation long cracks (a/W = 0·7) it is found for temperatures lower than −140°C that prior overload apparently increases the toughness. At higher temperatures there is a loss of toughness even though failure is cleavage dominated up to −80°C.

On the lower shelf at −170°C in the L-T orientation the fracture toughness variability after preloading is found (based on a sample of 14 specimens) to exhibit a bimodal distribution. This distribution is similar to that exhibited by non-preloaded material.     

STRUCTURAL COLLAPSE, THE FAILURE ASSESSMENT DIAGRAM AND "OFF-THE-SHELF" OPERATION

Abstract— The criteria for determining whether ferritic material exhibits fully ductile behaviour are generally based on the fracture toughness vs temperature relationship determined from standard laboratory test pieces (e.g. Charpy V Impact tests or fracture toughness specimens). This relationship defines a ductile-brittle transition region. When fully ductile, microvoid coalescence behaviour is experienced, and fracture toughness is described as being on the “upper shelf”. At “off the shelf” temperatures brittle, cleavage fracture is experienced. On the lower shelf the material is entirely brittle, exhibiting 100% crystallinity on the fracture face. As the temperature increases, initiation of tearing by microvoid coalescence occurs and some stable tearing can occur prior to the cleavage event. Material toughness increases with temperature until the upper shelf condition is achieved. The characteristics of fracture toughness tests in terms of the toughness level exhibited and the extent of ductile tearing experienced have been used as a guide to whether the structural application (e.g. a pressure vessel) will behave in a brittle or a ductile manner. This paper reports on a feasibility study where various worked examples have been performed to examine the concept of using a “cut off” on the failure assessment diagram, determined from the conditions required to cause plastic collapse of a pressure vessel, as a criterion for defining effective “ductile” operation. Fracture assessment procedures (R6 revisions 2 and 3 and PD6493 levels 2 and 3) have been utilised to determine the influence on pressure vessel performance of the behaviour of fracture toughness test specimens. The procedure of plotting a structural collapse “cut off” on a failure assessment diagram enables the assessment of whether a particular flaw geometry would result in gross deformation of the structure at failure. The use of this procedure provides an unambiguous demarcation between “fracture dominated” and “collapse controlled” conditions. This procedure facilitates judgements on the level of toughness necessary to ensure ductile operation and whether a “tearing plus toughness” requirement is necessary. It is recommended that consideration be given to including structural collapse into fracture assessment procedures carried out using R6 revision 3 or PD 6493:1991 procedures in order to determine the conditions when enhanced toughness no longer influences structural performance (i.e. when effective “upper shelf” conditions are attained).