Acyl exchange between oleoyl-CoA and phosphatidylcholine in microsomes of developing soya bean cotyledons and its role in fatty acid desaturation

Microsomes of developing soya bean cotyledons transfer oleate from oleoyl-CoA to phosphatidylcholine (PC) by two different mechanisms: one in which oleate transfer is accompanied by the release of free CoA and another which results in the exchange of oleate from oleoyl-CoA for unsaturated 18-carbon fatty acids of PC. The acyl exchange can be demonstrated only when bovine serum albumin is present in the incubation medium. ATP-dependent acyl-CoA synthetase is not involved in the exchange process, which apparently does not require any cofactors. In light of this exchange process, the oleate desaturase system was reinvestigated in order to determine what the actual substrate for this system is. Upon incubation of microsomes with high concentrations of [¹⁴C] oleoyl-CoA, bovine serum albumin and NADH, it could be conclusively demonstrated that most oleic acid is desaturated while part of the PC molecule. The amounts of [¹⁴C] linoleoyl-CoA formed could be explained entirely by the acyl exchange. The physiological significance of the acyl exchange system is discussed. A new method for separation of acyl-CoA from other lipids and free CoA using reversed phase column chromatography also is described.     

Difference algebra and system identification

The framework of differential algebra, especially Ritt’s algorithm, has turned out to be a useful tool when analyzing the identifiability of certain nonlinear continuous-time model structures. This framework provides conceptually interesting means to analyze complex nonlinear model structures via the much simpler linear regression models. One difficulty when working with continuous-time signals is dealing with white noise in nonlinear systems. In this paper, difference algebraic techniques, which mimic the differential-algebraic techniques, are presented. Besides making it possible to analyze discrete-time model structures, this opens up the possibility of dealing with noise. Unfortunately, the corresponding discrete-time identifiability results are not as conclusive as in continuous time. In addition, an alternative elimination scheme to Ritt’s algorithm will be formalized and the resulting algorithm is analyzed when applied to a special form of the nfir model structure.     

Highly Porous Geopolymers Through Templating and Surface Interactions

The natural porosity of geopolymers and the simplicity of geopolymer synthesis make them a potential candidate for the formation of highly porous ceramics. Here, the synthesis of highly porous (≈70 vol% or more) metakaolin geopolymer is demonstrated using a novel emulsion preparation with one‐pot curing and hydrophobization of the interior pores with alkylalkoxysilanes. Using mercury intrusion porosimetry, tailoring of the characteristic percolation pore size is demonstrated over a range of ≈200 nm to 10 μm. Using powder X‐ray diffraction, reactivity was shown to be decreased versus typical geopolymers, but substantial geopolymerization still occurred and the samples formed cohesive monoliths. Optional calcination under inert conditions allowed for formation of a glassy ceramic with a notable SiC phase, as well as further increasing the porosity by removing the hydrophobic pore coatings.     

Single-step formation of a biorecognition layer for assaying histidine-tagged proteins

The purpose of this work was to develop a simple procedure for the creation of a specific biorecognition layer for histidine-tagged (His-tagged) molecules. Such a layer was prepared by the spontaneous fusion of vesicles containing readily available plain (DOPC) and iminodiacetic acid (DOGS-NTA) phospholipids on a silica surface resulting in the formation of an NTA-containing supported lipid bilayer. The frequency surface acoustic waveguide device which supports Love waves was used to follow the real-time formation of the biorecognition layer. The mole percent of the DOGS-NTA phospholipids in the supported bilayer was optimized by following the kinetics of the fusion for the different NTA-containing lipids. Fluorescently labeled lipids were used with observations of the fluorescence recovery after photobleaching to confirm the presence of lipid bilayers. After saturating all NTA-molecules with Ni(2+), the binding of a His-tagged protein fragment within the concentration range of 0.04 and 0.4 mM to a 5 mol % DOGS-NTA/DOPC was detected; binding curves were used to calculate the apparent association constant k(on) = 2.56 x 10(4) M(-)(1) s(-)(1), dissociation constant k(off) = 1.3 x 10(-)(3) s(-)(1), and equilibrium constant k(eq) = 1.97 x 10(7) M(-)(1). The described method could find significant applications as a generic technique for preparing biorecognition layers for His-tagged proteins. In addition, the acoustic waveguide device, which provides high sensitivity together with flexibility in terms of the substrate material used, is shown to be an attractive alternative to direct optical biosensors.     

Output dead-beat control for nonlinear systems with one zero at infinity

The problem of output dead-beat control, i.e. one-step zeroing of the output, is considered for nonlinear systems with discrete-time controllers. The nonlinear system is assumed to have one zero at infinity. Conditions for the existence of the dead-beat control for small sampling intervals are derived. The closed-loop system is shown to be stable in the sense that all states converge to the origin if the controlled system is minimum phase.     

Relationship between plastic strains and microstructural parameters of AMg6 alloy under conditions of active tension and creep

We study the relationship between plastic strains and the density of dislocations in AMg6 alloy under conditions of quasistatic and combined tension, creep, and dynamic creep. It is shown that, in the presence of a cyclic component of loading, an increase in the level of plastic strains is well correlated with the increase in the density of dislocations in the material. The dependences aimed at the evaluation of the density of dislocations (plastic strains) in the material in the presence of the cyclic component of loading are proposed.Translated from Problemy Prochnosti, No. 6, pp. 48–58, November–December, 2004     

Fem prediction of the influence of warm prestressing on fracture toughness of heat-resistant steel

We present a procedure of prediction of the influence of warm prestressing combined with cycling on the brittle strength of steel 15Kh2MFA. Using a finite-element method, the effect of the combined warm prestressing on the stress-strain state at a fatigue crack tip is studied in an elastic-plastic statement. Electron microscopic observations of fracture surfaces have revealed that fracture is initiated at some distance from the fatigue crack front. Based on the pattern of influence of the plastic prestrain level on the cleavage stress of steel 15Kh2MFA and the experimental CID value, a method is put forward for finite-element modeling of the stress-strain state at a crack tip during the specimen fracture. Using the results of the finite-element modeling, the relevant curves have been plotted and an approximating formula has been proposed to represent the influence of the combined warm prestress level on the fracture toughness of steel 15Kh2MFA.     

An analog front end for v.22bis modems

An analog front end for the 2400-b/s v.22bis modem has been implemented in a 3-μm CMOS process. A high level of integration in the front end results in a low-cost, high-performance modem system. A mix of analog switched-capacitor and digital circuits is used throughout the chip. Some of the major functional blocks are a modulator, tone generator, band-split filters, programmable receive gain stage, 8-bit ADC (analog-to-digital converters), bandgap voltage reference, and special signal detectors. Features are included to support a number of lower-speed, split-band modem standards. The chip occupies 59000 mils ² and dissipates 200 mW. System and circuit aspects of the design are discussed; measured performance of the IC and of the complete modem system are given