Yeast sequencing reports. Isolation and sequence analysis of a gene from the linear DNA plasmid pPAC1–2 of Pichia acaciae that shows similarity to a killer toxin gene of Kluyveromyces lactis

The toxin-encoding linear plasmid systems found in Pichia acaciae and Kluyveromyces lactis yeasts appear to be quite similar, both in function and structural organization. By Southern hybridization, a linear plasmid of P. acaciae, pPac1–2, was found to hybridize to the second open reading frame (ORF2) of K. lactis plasmid pGKL1, known to encode the α and β subunits of the K. lactis toxin. A 1·7 kbp segment of pPac1–2 DNA was cloned, sequenced and shown to contain four regions of strong homology to four similarly oriented regions of K. lactis ORF2. This 1·7 kbp fragment also contained an ORF of 1473 bp that could encode a protein of ～ 55·8 kDa. Like the α subunit gene of K. lactis ORF2, a very hydrophobic region occurs at the N-terminus, perhaps representing a signal sequence for transport out of the cell. Unlike K. lactis ORF2, however, the encoded polypeptide is much smaller and lacks a recognizable domain common to chitinases. The structure of a toxin that includes the translation product of this P. acaciae ORF would likely be quite different from that of the K. lactis toxin. Analysis of the upstream region of the P. acaciae ORF revealed an upstream conserved sequence identical to that found before ORFs 8 and 9 of pGKL2. A possible hairpin loop structure, as has been described for each of the four K. lactis pGKL1 ORFs, was found just upstream of the presumed start codon. The similarity of the promoter-like elements found in the linear plasmid genes of these diverse yeasts reinforces the idea of the existence of a unique, but highly conserved, expression system for these novel plasmids. The sequence has been deposited in the GenBank data library under Accession Number U02596.     

Comparison of mainline and alternate approaches to fusion energy

The tokamak and tandem mirror concepts are compared with alternate confinement concepts using the criteria established in DOE/ET-0047, An Evaluation of Alternate Magnetic Fusion Concepts 1977. The concepts are evaluated and rated in each of three broad categories: confidence in physics and technology, and reactor desirability. The STARFIRE and MARS reactors are used as a basis for comparing the mainline tokamak and tandem mirror concepts with the alternate concepts evaluated in DOE/ET-0047. Two recent alternate concepts, theohmically heated toroidal experiment (OHTE) and thecompact reversed field pinch reactor (CRFPR), are also evaluated. Results indicate that the physics of the mainline tokamaks and tandem mirrors is better understood than that of most alternate concepts. Both mainline concepts rank near the middle for technology requirements, and both rank near or at the bottom when compared with the reactor desirability of alternate concepts.     

Liquid / liquid ion-transfer processes at the dioctylphosphoric acid (N,N-didodecyl-N',N'-diethylphenylenediamine) / water (electrolyte) interface at graphite and mesoporous TiO2 substrates

Biphasic electrode systems are studied for the case of the oxidation of the water-insoluble liquid N,N-didodecyl-N',N'-diethylphenylenediamine (DDPD) neat and dissolved in bis(2-ethylhexyl) phosphate (HDOP) and immersed in aqueous electrolyte media. The oxidation process in the absence of HDOP is accompanied by transfer of the anion (perchlorate or phosphate) from the water into the organic phase. However, in the presence of HDOP, oxidation is accompanied by proton exchange instead. This electrochemically driven proton exchange process occurs over a wide pH range. Organic microdroplet deposits of DDPD in HDOP at basal plane pyrolytic graphite electrodes are studied by voltammetric techniques and compared in their behavior to organic microphase deposits in mesoporous TiO2 thin films. The mesoporous TiO2 thin film acts as a host for the organic liquid and provides an alternative biphasic electrode system compared to the random microdroplet/graphite system. Two types of mesoporous TiO2 thin-film electrodes, (i) a 300-400-nm film on ITO and (ii) a 300-400-nm film on ITO sputter-coated with a 20-nm porous gold layer, are investigated.     

Altered glycosylation of the env-sea oncoprotein inhibits intracellular transport and transformation

The transforming gene product of the S13 avian erythroblastosis virus, env-sea, is a member of the growth factor receptor class of tyrosine kinases. The env-sea precursor protein gp155env-sea is proteolytically processed into the mature cleavage products gp85env and gp70env-sea, which are subsequently terminally glycosylated and transported to the cell surface. Previous studies have shown that the abnormal glycosylation of gp155env-sea induced by the carbohydrate processing inhibitor castanospermine blocks the proteolytic cleavage of gp155env-sea and impairs its transforming ability. We have shown recently that an uncleaved but fully glycosylated sea-encoded protein retains the ability to transform chicken embryo fibroblasts, indicating that proteolytic processing is not essential for transformation by the env-sea tyrosine kinase. To address the question of how castanospermine blocks transformation by env-sea, differential sucrose gradient centrifugation was performed on env-sea-transformed cells treated with the inhibitor. This report shows that no surface forms of env-sea could be detected in inhibitor-treated cells, suggesting that castanospermine acts by blocking the transport of sea-encoded proteins to the cell surface.     

"Shouldn't we consider...?": Jury discussions of forbidden topics and effects on damage awards.

The Federal Rules of Evidence prohibit disclosure to civil jurors of information that is arguably related to their decision-making (e.g., that either party is insured). The basis for so-called "blindfolding" is that a jury might be biased by this information to alter its appraisal of the evidence to reach a desired verdict. The purpose of this study was to examine the extent to which mock juries in an automobile negligence case discuss several "silent factors" during deliberation (viz., insurance carried by the parties, the payment of attorneys' fees, and previous settlements between the plaintiff and other defendants) and the effects of such discussion on their compensatory damage award. We presented summaries of the evidence that varied in the severity of the plaintiff's injuries and the reprehensibility of the defendant's conduct. These variables influenced judgments of liability and damage awards. Analysis of the content of jury deliberations regarding damages showed that, although nearly all juries talked about silent factors, the size of their damage awards was unrelated to the frequency of these discussions and that such discussion accounted for only a very small portion of the variance in awards. (PsycINFO Database Record (c) 2010 APA, all rights reserved)     

Orthonormal Chemical Reactions and Chemical Relaxation Part 4. Reactions in Solution with Concomitant Entropy and Volume Changes

The method described previously [2, 3] for obtaining the relaxation times, normal coordinates and normal modes of relaxation of a chemical system is extended to the case in which heat transfer and volume changes occur at rates too slow to maintain isothermal and isobaric conditions. The four types of chemical relaxation treated previously (isothermal and adiabatic relaxation at constant pressure and at constant volume) form limiting cases of this problem whose solution thus provides a generalization and unification of the concept of orthonormal chemical reactions.     

X-ray diffraction and resistivity studies of titanium-molybdenum alloys

The phase transformation behavior of the metastable beta phase in hydrogen charged Ti-Mo alloys was investigated using electrical resistivity and X-ray diffraction techniques. Hydrogen charging was found to have little effect on athermal omega phase formation in a highly susceptible alloy (16 wt pct Mo) but suppressed athermal omega in alloys with compositions near the critical composition for the transition to diffuse (incommensurate) type omega (∼20 wt pct Mo). No evidence was found for a hydrogen induced omega phase in a concentrated alloy (30 wt pct Mo). The incipient stages of the beta + beta’ phase separation in Ti-30 wt pct Mo were detected after aging slightly below the beta transus.     

Fresnel-integral approximation

In diffraction analysis, an approximation to K_-(x)=1/(√π)e^ix(2)+iπ/4∫_x ^∞e^-it(2) dt, (0-(x), suitable for computation with a hand-held computer, such as the HP-288