A high proportion of novel mutations in BRCA1 with strong founder effects among Dutch and Belgian hereditary breast and ovarian cancer families

We have identified 79 mutations in BRCA1 in a set of 643 Dutch and 23 Belgian hereditary breast and ovarian cancer families collected either for research or for clinical diagnostic purposes. Twenty-eight distinct mutations have been observed, 18 of them not previously reported and 12 of them occurring more than once. Most conspicuously, a 2804delAA mutation has been found 19 times and has never been reported outside the Netherlands. A common haplotype spanning > or = 375 kb could be identified for each of the nine examined recurrent mutations, indicating the presence of multiple BRCA1 founder mutations in the Dutch population. The 2804delAA mutation has been estimated to have originated approximately 32 generations ago. No specific breast or ovarian cancer phenotype could be assigned to any of the common mutations, and the ovarian cancer incidence among 18 families with the 2804delAA mutation was heterogeneous.     

Efficient temporal compression of coherent nanosecond pulses in acompact SBS generator-amplifier setup

A pulse compressor based on stimulated Brillouin scattering (SBS) in liquids is experimentally and theoretically investigated. It allows for the compression of Fourier-transform limited nanosecond pulses of several hundreds of millijoules of energy with both high conversion efficiency and a high temporal compression factor. The two-cell generator-amplifier arrangement is of a compact design not requiring external attenuation of the generator cell input energy. Pulses from an injection-seeded, frequency-doubled Nd:YAG laser of 300-mJ energy were compressed by a factor variable between 6 and 21 at up to 75% reflectivity. Deviation from unity SBS reflectivity is predominantly determined by optical component losses. The generation of 270-ps pulses with high beam quality was achieved in liquid methanol. These powerful pulses of variable duration are difficult to produce with common laser systems and are highly suited for the generation of high-harmonics in gases     

Compact CMOS constant-gm rail-to-rail input stage with gm-control by an electronic zener diode

A family of compact CMOS rail-to-rail input stages with constant-g _m is presented. To attain a constant-g_m over the whole common-mode input range, an electronic zener diode is inserted between the tails of the complementary input pairs. This zener keeps the sum of the gate-source voltages of the input pairs, and therefore the g _m of the rail-to-rail input stage, constant. Two possible implementations of the zener have been realized and inserted in a rail-to-rail input stage. These input stages are implemented in two two-stage compact amplifiers. Both amplifiers have been realized in a 1 μm BiCMOS process. They have a unity-gain frequency of 2-MHz, for a capacitive load of 20 pF     

Pulse compression to the subphonon lifetime region by half-cyclegain in transient stimulated Brillouin scattering

A new approach to the transient pulse compression by stimulated Brillouin scattering (SBS) is presented. The theoretical analysis involving the time-dependent SBS gain in explicit form leads to a nonlinear system of partial differential equations, solved numerically by a generalization of the split-step method. It is shown theoretically and confirmed experimentally that the phonon lifetime is not always an appropriate parameter that determines the lower limit to the pulse duration in SBS compressors. A half-cycle gain regime is found for pulses shorter than the phonon lifetime. Hence, under proper conditions, pulses as short as half the acoustic period can be produced     

Development and application of an electron spin resonance spectrometry method for the determination of oxygen free radical formation by particulate matter

Exposure to increased levels of ambient particulate matter (PM) are associated with several health effects, including cardiopulmonary diseases. The formation of reactive oxygen species (ROS) is thought to play an important role in the induction of these health effects. To quantify the ROS generating capacityof PM,we developed an improved electron spin resonance (ESR) spectrometry-based method. ROS formation was measured directly on PM-containing filters, thereby avoiding the selective extraction of components and loss of material or reactivity, which is likely to occur during filter extraction. Also, ascorbic acid was added to stimulate ROS formation. This method was applied to PM10 samples originating from different sources. The radical generating capacity of PM10 from both gasoline and diesel engine exhaust was significantly higher as compared to that of PM10 from ambient or indoor air. Furthermore, in urban PM10 and PM2.5, ROS-generating capacity significantly correlated with concentrations of polycyclic aromatic hydrocarbon content and particular transition metals. This indicates thatthis improved ESR method may be a valuable tool for evaluating the relationship between ROS formation by PM and the adverse health effects associated with this type of air pollution.     

CMOS low-voltage operational amplifiers with constant-gm rail-to-rail input stage

Two 3.3-V operational amplifiers with constant-g _m rail-to-rail input stage and rail-to-rail output stage are presented. The constant transconductance (g _m ) ensures a constant unity-gain frequency within the whole commonmode input range. Two new methods to control theg _m are introduced. Both operational amplifiers use the same rail-to-rail output stage. The operational amplifiers have been integrated in a CMOS semicustom process with transistor lengths of 10µm. The common-mode input voltage swing extends beyond the positive supply rail by 400 mV and beyond the negative supply rail by 200 mV. The output voltage is able to reach within 130 mV of the supply rails. The output current of the operational amplifiers is 2 mA and the voltage gain is 85 dB. The unity-gain frquency is 165 kHz, which is mainly limited by the relatively long transistor lengths of 10µm. In another process with channel lengths of 2µm, simulation results showed that a unity-gain frequency of 4 MHz can easily be obtained.