Job satisfaction and job performance: A meta-analysis.

Coded 9 variables in a meta-analysis of 74 empirical studies of job satisfaction–job performance. Aggregated studies had an S sample size of 12,192 and 217 satisfaction–performance correlations. Findings show that (1) the best estimate of the true population correlation between satisfaction and performance was relatively low (.17); (2) much of the variability in results obtained in previously research was due to the use of small sample sizes, while unreliable measurement of the satisfaction and performance constructs has contributed relatively little to this observed variability in correlations; and (3) the 9 variables coded (composite vs unidimensional criteria, longitudinal vs cross-sectional measurement of performance relative to satisfaction, the nature of the performance measure, self-reports vs other sources, use of specific performance measures, subjectivity or objectivity of measures, specific-facet satisfaction vs global satisfaction, well-documented vs researcher-developed measurement, and white-collar vs blue-collar) were only modestly related to the magnitude of the satisfaction–performance correlation. (3 p ref) (PsycINFO Database Record (c) 2010 APA, all rights reserved)     

Full-Spectrum Targeted Mutagenesis in Plant and Animal Cells

Directed evolution is a powerful approach for protein engineering and functional studies. However, directed evolution outputs from bacterial and yeast systems do not always translate to higher organisms. In situ directed evolution in plant and animal cells has previously been limited by an inability to introduce targeted DNA sequence diversity. New hypermutation tools have emerged that can generate targeted mutations in plant and animal cells, by recruiting mutagenic proteins to defined DNA loci. Progress in this field, such as the development of CRISPR-derived hypermutators, now allows for all DNA nucleotides within user-defined regions to be altered through the recruitment of error-prone DNA polymerases or highly active DNA deaminases. The further engineering of these mutagenesis systems will potentially allow for all transition and transversion substitutions to be generated within user-defined genomic windows. Such targeted full-spectrum mutagenesis tools would provide a powerful platform for evolving antibodies, enzymes, structural proteins and RNAs with specific desired properties in relevant cellular contexts. These tools are expected to benefit many aspects of biological research and, ultimately, clinical applications.