Mutations in the N-terminal globular domain of the type X collagen gene (COL10A1) in patients with Schmid metaphyseal chondrodysplasia

STATEMENT OF PROBLEM: Improved dental stone has been widely used for producing dies for the fabrication of restorations with the lost-wax technique. Improved dental stone is used for several reasons, but it is selected most often because of its reasonable cost, ease of use, and ability to produce consistent results. PURPOSE: This study evaluated the ability of an epoxy resin die material and a type IV dental stone to dimensionally reproduce a custom-fabricated metal die. MATERIAL AND METHODS: Dies were fabricated and measurements were made from three reference lines. Measurements were repeated three times for the master die and for the specimen dies. A mean measurement and percent relative change for each dimension was calculated. RESULTS: A significant difference in the relative change for die height was found between the groups studied (p < 0.003). CONCLUSIONS: This epoxy die system will provide a degree of dimensional accuracy comparable to gypsum when used with addition silicone impression material.     

Potential bile acid metabolites. 24. An efficient synthesis of carboxyl-linked glucosides and their chemical properties

A facile and efficient synthesis of the carboxyl-linked glucosides of bile acids is described. Direct esterification of unprotected bile acids with 2,3,4,6-tetra-O-benzyl-d-glucopyranose in pyridine in the presence of 2-chloro-1,3,5-trinitrobenzene as a coupling agent afforded a mixture of the α- and β-anomers (ca. 1∶3) of the 1-O-acyl-d-glucoside benzyl ethers of bile acids, which was separated effectively on a C₁₈ reversedphase chromatography column (isolated yields of α- and β-anomers are 4–9% and 12–19%, respectively). Subsequent hydrogenolysis of the α- and β-acyl glucoside benzyl ethers on a 10% Pd−C catalyst in acetic acid/methanol/EtOAc (1∶2∶2, by vol) at 35°C under atmospheric pressure gave the corresponding free esters in good yields (79–89%). Chemical specificities such as facile hydrolysis and transesterification of the acyl glucosides in various solvents were also discussed.     

Refined mapping of a gene responsible for Fukuyama-type congenital muscular dystrophy: evidence for strong linkage disequilibrium

Fukuyama-type congenital muscular dystrophy (FCMD), the second most common form of childhood muscular dystrophy in Japan, is an autosomal recessive severe muscular dystrophy associated with an anomaly of the brain. After our initial mapping of the FCMD locus to chromosome 9q31-33, we further defined the locus within a region of approximately 5 cM between loci D9S127 and CA246, by homozygosity mapping in patients born to consanguineous marriages and by recombination analyses in other families. We also found evidence for strong linkage disequilibrium between FCMD and a polymorphic microsatellite marker, mfd220, which showed no recombination and a lod score of (Z) 17.49. A "111-bp" allele for the mfd220 locus was observed in 22 (34%) of 64 FCMD chromosomes, but it was present in only 1 of 120 normal chromosomes. This allelic association with FCMD was highly significant (chi 2 = 50.7; P < .0001). Hence, we suspect that the FCMD gene could lie within a few hundred kilobases of the mfd220 locus.     

Numerical analysis of two-dimensional compressible turbulent flows in a turbine stage

A numerical analysis based on the compressible Reynolds-averaged Navier-Stokes equation has been developed for the analysis of two-dimensional compressible turbulent flows in a turbine stage (nozzle and bucket). In the present flow analysis, governing equations are solved by the use of a time dependent explicit method and a two-equation model of turbulence is employed to estimate turbulence effects. To calculate nozzle and bucket flow fields simultaneously, a steady interaction between these flows is assumed. For spatial discretization of the governing equations, a control volume method combined with a body-fitted curvilinear coordinate system is developed to calculate the flows in arbitrarily shaped cascades. In order to assure the effectiveness of the present method, computations are carried out for a two-dimensional section at a blade midspan in a turbine stage. The method gives satisfactory results about boundary layers on blade surfaces, nozzle wake profiles and pitchwise averaged turbine design parameters at each blade exit.     

Sliding window protocol with selective-repeat ARQ: performance modeling and analysis

A sliding window mechanism is well known to be a convenient way to integrate cumulative acknowledgement and flow control functions in a simple manner. Hence, widely used reliable data-transfer protocols such as TCP-SACK and RLC utilize this sliding window mechanism in conjunction with a selective-repeat ARQ (automatic repeat request) function, namely, the selective-repeat sliding window protocol (SR-SWP). We demonstrate that SR-SWP yields a throughput restraint effect (TORE) in particular situations when packet losses occur. We propose an analytical performance model to represent the TORE in the case of heavy traffic. Using this model, we derive explicit forms of goodput and mean resequencing delay, which are fundamental qualities of service parameters. From numerical results, we demonstrate that the impact of TORE on the performance is not negligible for high packet-loss probabilities. Furthermore, we show that the SR-SWP model exhibits the lower goodput and the smaller mean resequencing delay than those obtained from the traditional SR-ARQ protocol model.     

Synthesis of bile acid 24-acyl glucuronides

The role of a surgeon is a complex one. A variety of skills and abilities in a different array of performance tasks is required before he can be competent in his job. As such, there is no simple rule of thumb that can be used to assess a surgeon's performance. As performance assessment and appraisal become increasingly important both for manpower decisions and personal development, it is important that we recognise the different roles a surgeon plays. Only then are we able to subdivide a surgeon's job demands into small manageable portions for analysis. This paper examines the multi-faceted aspects of a surgeon's job, and how each facet should be individually assessed and appraised.     

A new finite element technique for the analysis of steady viscous flow problems

A new finite element technique for two-dimensional viscous incompressible fluid flow problems is presented in this paper. The vorticity transport equation is integrated in a small control volume, which results in the conservation law of vorticity. The finite element technique is applied to this equation together with the continuity equation, where simple linear triangular elements with three nodes are used for the formulation. Resulting sets of algebraic equations are solved by the use of a kind of relaxation method. Numerical results for viscous flow past a cavity show good agreement with experimental results.     

Collaborative work to determine the optimal administration period and parameters to detect drug effects on male rat fertility--study on estradiol benzoate effects

In order to examine the optimal administration period and parameters for male fertility assessment, male rats were subcutaneously administered 0.2, 2 or 20 micrograms/kg of estradiol benzoate (E2B), a known testicular toxicant, for 4 weeks or 9 weeks before mating. After 4 weeks administration, suppression of body weight gain and food consumption, decreases in prostate and seminal vesicle weights, atrophy of Leydig cells, and mature spermatid retention at stages IX, X and XI were observed in the 2 and 20 micrograms/kg groups. In the 20, micrograms/kg group, decreases in epididymides weight and copulation index were also found but the number of sperm and sperm motility were not affected. In the 0.2 micrograms/kg group, no changes were noted in any parameters. After 9 weeks administration, decreases in testis weight and the number and motility of sperm were observed in the 20, micrograms/kg group, in addition to the changes found after 4 weeks administration. These results suggest that detailed histopathological evaluation and determination of accessory sex organ weights are sensitive for evaluating the effects of E2B on male fertility. Results with the 4-weeks treatment were comparable to those with the 9-weeks treatment in terms of these parameters.     

Effects of Size and Distribution of Spheroidized Cementite on Void Initiation in the Punched Surface of Medium-Carbon Steel

Metallurgical and Materials Transactions A - In this study, we investigated the effects of the size and distribution of spheroidized cementite on the characteristics of a punched surface as well as...