The use of monoclonal antibodies for detecting the influenza virus

The possibilities of using influenza A (Leningrad) 385/80 (H3N2) virus matrix protein-specific FITC-labeled D8 monoclonal antibodies in immunofluorescence assays were investigated. The virus antigen accumulation was detected in chorioallantoic cells of chick embryos. Exhibiting the type-specific properties, the fluorescent antibodies stain the perinuclear space, cytoplasmic membrane, and granular structures in the cytoplasm of infected cells. The haemagglutination test tires in the corresponding specimens were at least 1:16.     

Wounds of the heart

The frequency of wounds of the heart as the cause of lethal outcomes on the battle-field practically has not changed during the recent 50 years and is equal to 80%. At the same time, the danger of such complications as infectious endocarditis has increased. The author's experience with surgical treatment of gunshot wounds of the heart makes 85 cases. Symptoms of intracardiac infection were detected in 74% of the wounded, 52 of them having the septic form. Operations were performed under artificial circulation. They included the extraction of foreign bodies, complex sanitation of the heart chambers and recovery of intracardiac hemodynamics. There were no lethal outcomes in the nearest postoperative period. Success of the treatment depends on terms of delivery of the patient to the hospital, quickness of surgical intervention and adequate intensive therapy.     

The debatable aspects of the adhesive syndrome

The commissural intestinal obstruction and commissural disease are two variants of the commissural syndrome characterized by a complete or partial disturbance of the intestinal transit. The data of 390 clinical observations have shown that choice of methods of treatment must be based on the topical diagnosis of the syndrome such as the establishment of the place of obstruction and assessment of the degree of the intestinal transit disturbance. In the both cases a new roentgenological technique of investigation can be used with contrast labels. The new approaches to medical tactics allowed the time of diagnosing the commissural syndrome to become shorter and to considerably improve the direct results of the operative procedures.     

The clinical aspects of the aftermath of the accident (based on data from the Military Medical Academy)

The results of medical examinations of the persons taking part in elimination of consequences of Chernobyl's disaster obtained by the specialists of Military-medical academy allowed to verify the conception of damage action of radiation and nonradiation complex of injury factors, to estimate well-information of stale and new biological indicators of radiation dose, to plan the ways of rescuers rehabilitation during stationary, polyclinic and sanatorium stages of treatment.     

Subglottic hemangioma as the cause of laryngostenosis in young children

The amount of plasma cells synthesizing different class immunoglobulins in the gallbladder wall in 9 practically healthy people and in 19 patients with different forms of cholecystitis was studied by a complex of histological, histochemical and immunofluorescent methods. It was established that catarrhal cholecystitis was accompanied by activated production of immunoglobulins of all classes by plasmocytes of the gallbladder wall. In patients with destructive forms of cholecystitis the level of secretory immunoglobulin A was substantially decreased as well as the amount of plasmocytes synthesizing IgA. The amount of immunocytes producing IgM and IgG became disproportionally greater, there appeared immune IgM complexes and IgG and IgG in the wall of blood vessels and perivascular stroma. The author considers that local immune reactions play an important role in protection of the gallbladder mucosa and pathomorphogenesis of cholecystitis.     

A comparative assessment of the results of surgical and conservative treatments in parkinsonism

Late outcomes of surgical and medical treatment of parkinsonism were studied in 582 (including 321 medical and 261 surgical) patients. The follow-up ranged from 6 to 30 years. Three hundred and fifty eight surgeries were performed. In immediate postoperative period, the following results were observed: significant (64%), moderate (20.3%) improvements, no changes (8%), deterioration (3.1%). Late outcomes were as follows: significant (34.9%), moderate (20.3%) improvements, no changes (28.7%), deterioration (16.1%). In surgical patients the complication rate was 14.8%, mortality 0.8%. Analysis of outcomes of surgical and medical treatment showed a statistically significant predominance of good outcomes in surgical patients (34%) as compared with medical ones (14%).