Crystal transformation from the α- to the β-form upon tensile drawing of poly(l-lactic acid)

A film of poly(l-lactic acid) (PLLA) consisting of highly oriented α crystals was uniaxially drawn by tensile force. The effects of the draw ratio (DR), draw temperature (T_d), and draw stress on the crystal/crystal transformation from the α- to the β-form crystals were studied. At the initial stage of drawing, the highly oriented α crystals of the starting film transformed into a broader orientation distribution, and significant crystal disorder was introduced. Upon further drawing, the α crystals steadily transformed into β crystals with increasing the DR. For the drawing at a constant T_d, the crystal transformation proceeded more efficiently at a higher draw rate and, hence, at a higher draw stress. Furthermore, for the drawing at a constant draw rate, the transformation proceeded with DR most efficiently for the tensile draw at a T_d around 140 °C, although the draw stress increased with decreasing the T_d. The present result combined with the previous finding in the drawing of PLLA by solid-state extrusion [Macromolecules 36 (2003) 3601] suggests that there is a T_d of around 140 °C at which the crystal transformation proceeds most efficiently with DR, suggesting that there are two factors that have opposite effects on the efficiency of the crystal transformation with increasing the T_d. However, as a result of the combined effects of the T_d and DR on the crystal transformation and the ductility increase with the T_d, an oriented film consisting predominantly of β crystals was obtained by tensile drawing at a T_d in the range of 140-170 °C to the highest DR achieved at each T_d.     

An algorithm for similar utterance section extraction for managing spoken documents

This paper proposes a new, efficient algorithm for extracting similar sections between two time sequence data sets. The algorithm, called Relay Continuous Dynamic Programming (Relay CDP), realizes fast matching between arbitrary sections in the reference pattern and the input pattern and enables the extraction of similar sections in a frame synchronous manner. In addition, Relay CDP is extended to two types of applications that handle spoken documents. The first application is the extraction of repeated utterances in a presentation or a news speech because repeated utterances are assumed to be important parts of the speech. These repeated utterances can be regarded as labels for information retrieval. The second application is flexible spoken document retrieval. A phonetic model is introduced to cope with the speech of different speakers. The new algorithm allows a user to query by natural utterance and searches spoken documents for any partial matches to the query utterance. We present herein a detailed explanation of Relay CDP and the experimental results for the extraction of similar sections and report results for two applications using Relay CDP. Yoshiaki Itoh has been an associate professor in the Faculty of Software and Information Science at Iwate Prefectural University, Iwate, Japan, since 2001. He received the B.E. degree, M.E. degree, and Dr. Eng. from Tokyo University, Tokyo, in 1987, 1989, and 1999, respectively. From 1989 to 2001 he was a researcher and a staff member of Kawasaki Steel Corporation, Tokyo and Okayama. From 1992 to 1994 he transferred as a researcher to Real World Computing Partnership, Tsukuba, Japan. Dr. Itoh's research interests include spoken document processing without recognition, audio and video retrieval, and real-time human communication systems. He is a member of ISCA, Acoustical Society of Japan, Institute of Electronics, Information and Communication Engineers, Information Processing Society of Japan, and Japan Society of Artificial Intelligence. Kazuyo Tanaka has been a professor at the University of Tsukuba, Tsukuba, Japan, since 2002. He received the B.E. degree from Yokohama National University, Yokohama, Japan, in 1970, and the Dr. Eng. degree from Tohoku University, Sendai, Japan, in 1984. From 1971 to 2002 he was research officer of Electrotechnical Laboratory (ETL), Tsukuba, Japan, and the National Institute of Advanced Science and Technology (AIST), Tsukuba, Japan, where he was working on speech analysis, synthesis, recognition, and understanding, and also served as chief of the speech processing section. His current interests include digital signal processing, spoken document processing, and human information processing. He is a member of IEEE, ISCA, Acoustical Society of Japan, Institute of Electronics, Information and Communication Engineers, and Japan Society of Artificial Intelligence. Shi-Wook Lee received the B.E. degree and M.E. degree from Yeungnam University, Korea and Ph.D. degree from the University of Tokyo in 1995, 1997, and 2001, respectively. Since 2001 he has been working in the Research Group of Speech and Auditory Signal Processing, the National Institute of Advanced Science and Technology (AIST), Tsukuba, Japan, as a postdoctoral fellow. His research interests include spoken document processing, speech recognition, and understanding.     

Solving constraint-satisfaction problems by a genetic algorithm adopting viral infection

Several approximate algorithms have been reported to solve large constraint-satisfaction problems (CSPs) within a practical time. While those papers discuss techniques to escape from local optima, this paper describes a method that actively performs global searches. The present method improves the rate of search of genetic algorithms by using viral infection instead of mutation. Partial solutions of a CSP are considered to be viruses, and a population of viruses is created, as well as a population of candidate solutions. The search for a solution is conducted by crossover and infection. Infection substitutes the gene of a virus for the locus decided by the virus. Experimental results using randomly generated CSPs prove that the proposed method is faster that usual genetic algorithms at finding a solution when the constraint density of a CSP is low.     

Photo-induced properties of anodic oxide films on Ti6Al4V

Photocatalytic activity and wettability of the anodic oxide layer on Ti6Al4V prepared by anodization in a sulfuric acid electrolyte are explored. The oxide is composed mainly of TiO₂ with V₂O₅, VO₂ and Al₂O₃. The crystal structure of the TiO₂ varies from anatase to rutile with the sulfuric acid concentration in the electrolyte. Anatase exhibits better photocatalytic activity compared with rutile, which is different from those on Ti and Ti-Nb-Sn alloy. Contact angles of the oxides decrease with ultraviolet light illumination, and hydrophilicity is observed in the rutile oxide. Both photocatalytic activity and hydrophilicity are inferior to the corresponding Ti and Ti-Nb-Sn alloy, which is explained by the presence of Al₂O₃ in the anodic oxide.     

Thermodynamic analysis for the controllability of elements in the recycling process of metals

This study presents the results of chemical thermodynamic analysis on the distribution of elements in the smelting process of metallic materials to examine the controllability of impurities in the pyrometallurgical technique. The results of the present work can give an answer against the frequently given question; "Which impurity element can be removable in metallurgical process?" or "How far can the impurity level be controlled?". The proposed method was applied to estimate the distribution of 29 elements for a copper converter and 26 elements for a steel-making process and shows the distribution tendency of elements among the gas, slag, and metal phases as well as clarifying which metals can be recovered or removed from secondary resources in metallurgical processes. The effects of temperature, oxygen partial pressure, and slag composition on the distribution ratio of elements were also evaluated, and the removal limit or controllability of impurity in these two processes was presented. This study results in thermodynamic features of various elements in the pyrometallurgical process and also shows, even by varying process parameters such as temperature and oxygen partial pressure, no drastic improvement of removal efficiency should be expected, except for lead and tin in copper.     

Improvement of productivity of active horseradish peroxidase in Escherichia coli by coexpression of Dsb proteins

Coexpression of two classes of folding accessory proteins, molecular chaperones and foldases, can be expected to improve the productivity of soluble and active recombinant proteins. In this study, horseradish peroxidase (HRP), which has four disulfide bonds, was selected as a model enzyme and overexpressed in Escherichia coli. The effects of coexpression of a series of folding accessory proteins (DnaK, DnaJ, GrpE, GroEL/ES, trigger factor (TF), DsbA, DsbB, DsbC, DsbD, and thioredoxin (Trx)) on the productivity of active HRP in E. coli were examined. Active HRP was produced by very mild induction with 1 μM isopropyl-β- -thiogalactopyranoside (IPTG) at 37°C, whereas the amount of active HRP produced by the induction with 1 mM IPTG was negligibly small. Active HRP production was increased significantly by coexpression of DsbA-DsbB (DsbAB) or DsbC-DsbD (DsbCD), while coexpression of molecular chaperones did not improve active HRP production. The growth of E. coli cells was inhibited significantly by the induction with 1 mM IPTG in a HRP single expression system. In contrast, when HRP was coexpressed with DsbCD, the growth inhibition of E. coli was not observed. Therefore, coexpression of Dsb proteins improves both the cell growth and the productivity of HRP.     

Effects of an electric blanket on sleep stages and body temperature in young men

The aim of this study was to investigate any effects of electric blanket on sleep stages and body temperature. Nine male subjects slept under two conditions: using the electric blanket (HB); and not using the electric blanket (C). The ambient condition was controlled at 3 degrees C relative humidity 50-80%. Electroencephalography, electrooculography (EOG) and electromyography, rectal temperature, skin temperature and microclimate temperature and humidity were recorded continuously through the night. Body weight was measured before and after sleep. The amount of stage 1 and number of stage 1 and rapid eye movement sleep decreased in HB compared to C. No significant difference was observed in other sleep stages. Rectal temperature was higher in HB compared to C. The thigh, leg and foot skin temperature was higher in HB than C. The microclimate temperature of the foot area was higher in HB compared to C. No significant difference was observed in whole body sweat loss between the conditions. These results suggest that use of an electric blanket under low ambient temperature may decrease cold stress to support sleep stability and thermoregulation during sleep.     

α-tocopherol affects the urinary and biliary excretion of 2,7,8-trimethyl-2(2′-carboxyethyl)-6-hydroxychroman, γ-tocopherol metabolite, in rats

In this study, we investigated a change in the excretory content of 2,7,8-trimethyl-2(2′-carboxyethyl)-6-hydroxychroman (γ-CEHC), a γ-tocopherol (γ-Toc) metabolite, in rat urine and bile by using a new high-performance liquid chromatography-elelectrochemical detection (HPLC-ECD) method. In this determination, CEHC [α- and γ-CEHC, where α-CEHC-2,5,7,8-tetramethyl-2(2′-carboxyethyl)-6-hydroxychioman] in the biological specimens were treated with 3 N methanolic HCl to hydrolyze conjugates and to promote esterification. The methylated samples were extracted by n-hexane/water (1∶2). The analyses of the methyl esters of α-CEHC and γ-CEHC were performed by an HPLC-ECD using an ODS-3 column at 35°C. The mobile phase was acetonitrile/water (45∶55, vol/vol) containing 50 mM sodium perchlorate. After rat urine and bile samples, respectively, were methylated as described above, methylated biliary metabolites were identified by liquid chromatography mass spectrometry as methyl esters of γ-CEHC. Furthermore, we examined the differences in the excretion of γ-CEHC between rat urine and bile after an oral administration of γ-Toc or α- +γ-Toc by the above HPLC method. In the γ-Toc group, each vitamin E-deficient rat was given 0.5 mL of a stripped corn oil preparation containing 10 mg of γ-Toc. In the α- +γ-Toc group, the rat was given 10 mg of α-Toc and 10 mg of γ-Toc. The content of γ-CEHC in rat urine from the α- +γ-Toc group was increased more in comparison to the γ-Toc group at 18–36 h after oral administration. Moreover, the content of γ-CEHC in rat bile in the α- +γ-Toc group was increased more in comparison to the γ-Toc group at 6–18 h after oral administration. Therefore, we have suggested that γ-CEHC was shifted mainly to urinary excretion after γ-CEHC had been excreted into the bile. Furthermore, we assume that α-Toc may affect the metabolism of γ-Toc to γ-CEHC in the body.     

Dietary Lipids Impacts on Healthy Ageing

Healthy ageing is gaining attention in the lipid nutrition field. As in vivo biomarkers of healthy ageing, we have evaluated the survival, learning/memory performance, and physical potencies in rodents fed a diet supplemented with high-linoleic acid (LNA, ω6) safflower oil or high-α-linolenic acid (ALA, ω3) perilla oil for long periods. The results suggested that perilla oil with a low ω6/ω3 ratio is beneficial for healthy ageing. In order to address this issue further, we determined the survival of stroke-prone SHR (SHRSP) rats fed a conventional rodent diet supplemented with 10% fat or oil. Survival was longer with ω3-rich oils compared with ω6-rich oils. However, some kinds of vegetable oils and hydrogenated oils shortened the survival of SHRSP rats to an unusual degree (ca. 40% compared with that of ω6-rich oil) that could not be accounted for by the fatty acid and phytosterol composition of the oils. The observed decrease in platelet counts was associated with pathological changes in the kidney and other organs. Dihydro-vitamin K1 is proposed as a likely candidate as a stroke-stimulating factor in hydrogenated oils. Thus, factors other than fatty acids (ω6/ω3 balance) and phytosterols must be taken into account when fats and oils are evaluated in relation to healthy ageing.     

C-terminus engineering of soybean proglycinin: improvement of emulsifying properties

Introduction of the extension region of beta-conglycinin alpha' subunit at the C-terminus of proglycinin A1aB1b results in the improvement of its emulsifying properties. To understand the basic for such improvement, we introduced the alpha' and alpha extension regions to the A2B1a C-terminus, and the alpha extension and A5A4B3 hypervariable regions, and an oligopeptide composed of 20 negatively or positively charged residues to the A1aB1b C-terminus, creating A2B1aalpha', A2B1aalpha, and A1aB1balpha, A1aB1bA4IV, A1aB1bNeg and A1aB1bPos, respectively. All the modified versions were produced in Escherichia coli. Their molecular size, thermal stability, surface hydrophobicity, solubility and emulsifying ability were studied. Analyses of molecular size and thermal stability suggested that all the modified versions formed the proper conformation similar to that of the wild type (WT). Solubility was intrinsic to each mutant. At ionic strength 0.5, the emulsifying abilities of all mutants were better than that of the WT except A1aB1bPos and A1aB1bNeg, and at ionic strength 0.08, all mutants especially A1aB1bPos exhibited better emulsifying ability than did the WT. The order of stability of the emulsion at both ionic strengths (0.08 and 0.5) was A1aB1balpha >or= A2B1aalpha > A1aB1balpha' >or= A2B1aalpha' > A1aB1bPos > A1aB1bA4IV >or= A1aB1bNeg > A1aB1b, A2B1a. These results indicate that the emulsion stability of proglycinin mutants depends on length and hydropathy profile of the polypeptides added to the C-terminus of proglycinin.