Oral administration of rapamycin and cyclosporine differentially alter intestinal function in rabbits

The immunosuppressive drugs rapamycin (Rap) and cyclosporine A (CsA) are used clinically to modify or abolish immune-mediated functions. This study examined the effect of orally administered regimens of Rap, CsA, and a combination of Rap/CsA on intestinal function in male New Zealand white rabbits. Animals received oral doses of CsA (15 mg/kg/body weight/day), low-dose (LD) and high-dose (HD) Rap (0.25 or 1 mg/kg/body wt/day, respectively), or Rap/CsA (0.25 and 5 mg/kg/body wt/day, or 0.5 and 5 mg/kg/body wt/day, respectively) for 20 days. We measured in vitro uptake of nutrients and permeability, and morphometric measurements in the jejunum and ileum were made. Animals receiving HD-Rap or HD-Rap/CsA had decreased food intake, body weight, and intestinal weight, when compared with LD-Rap, LD-Rap/CsA, CsA, or controls. The maximal transport rate (Vmax) for the active jejunal uptake of D-glucose was increased in HD-Rap and CsA, but not in the HD-Rap/CsA-treated animals. The jejunal Vmax of D-glucose in the LD-Rap- or -Rap/CsA-treated animals was no different from controls. In the HD-Rap- and HD-Rap/ CsA-treated animals, jejunal rates of uptake of stearic, linoleic, and linolenic acids were reduced when compared with controls. Jejunal and ileal permeability (as assessed by the passive uptake of L-glucose, tissue conductance, and mucosal-to-serosal flux of [3H]inulin) was increased in animals treated with HD-Rap or HD-Rap/CsA, when compared with CsA or controls. These parameters of permeability were no different at lower doses of Rap or Rap/CsA. The jejunal and ileal villous surface area was increased in CsA, but decreased in HD-Rap or HD-Rap/CsA animals. Thus, HD-Rap given alone or in combination with CsA reduced body weight gain, in part due to reduced food intake and malabsorption of lipids, which was due at least in part to reduced intestinal surface area. The relevance of these findings to patients undergoing chronic immunosuppressive drug therapy needs to be established.     

Fish oil prevents change in arachidonic acid and cholesterol content in rat caused by dietary cholesterol

Rats were fed diets high in either saturated fat (beef tallow) or α-linolenic acid (linseed oil) or eicosapentaenoic and docosahexaenoic acids (fish oil) with or without 2% cholesterol supplementation. Consumption of linseed oil and fish oil diets for 28 days lowered arachidonic acid content of plasma, liver and heart phospholipids. Addition of 2% cholesterol to diets containing beef tallow or linseed oil lowered 20∶4ω6 levels but failed to reduce 20∶4ω6 levels when fed in combination with fish oil. Feeding ω3 fatty acids lowered plasma cholesterol levels. Addition of 2% cholesterol to the beef tallow or linseed oil diet increased plasma cholesterol concentrations but not when fish oil was fed. Feeding the fish oil diet reduced the cholesterol content of liver, whereas feeding the linseed oil diet did not. Dietary cholesterol supplementation elevated the cholesterol concentration in liver in the order: linseed oil > beef tallow > fish oil (8.6-, 5.5-, 2.6-fold, respectively). Feeding fish oil and cholesterol apparently reduced 20∶4ω6 levels in plasma and tissue lipids. Fish oil accentuates the 20∶4ω6 lowering effect of dietary cholesterol and appears to prevent accumulation of cholesterol in plasma and tissue lipids under a high dietary load of cholesterol.     

Characteristics of college cyberbullies

Cyberbullying is a newer variation of bullying that utilizes technology to harass. This study investigated the psychological symptomology, suicidal behaviors, aggressive tendencies, and illegal behaviors of college cyberbullies. Sixty cyberbullies and 19 cyberbully/victims (participants who cyberbullied and were victims of cyberbullying) scored higher in psychological symptoms of depression, hostility, interpersonal sensitivity, paranoia, phobic anxiety, and psychoticism than participants who were uninvolved with cyberbullying. The cyberbullies also endorsed more suicidal behaviors and scored higher in aggression. The cyberbully/victims engaged in more violent and drug crimes than cyberbullies and controls. These findings indicate perpetrators of cyberbullying have more psychological distress, aggressive tendencies, and engage in more illegal behaviors than those who do not cyberbully. This is the first study to show the distress cyberbullies are experiencing.     

利用准则实现校准图像质量评测

利用软件工具定量分析数字成像系统的性能可以优化整体图像质量 从手机相机到便携式摄像机,数字成像技术在人们的生活中无处不住。尽管消费者的选择越来越多,但现在仍没有标准化的客观评价量度来定量分析这些设备的整体性能。目前已有数种由国际标准组织（ISO）制定的标准可以对性能的几个不同方面予以量化,     

Attachment style and relationship satisfaction: Test of a self-disclosure explanation.

[Correction Notice: An erratum for this article was reported in Vol 30(2) of Canadian Journal of Behavioural Science/Revue canadienne des sciences du comportement (see record 2009-19933-001). Some figures were inadvertently omitted in two tables. The correct tables are provided in the erratum.] Explored a self-disclosure explanation for why those with a secure attachment style report greater relationship satisfaction than those with insecure attachment styles. Ss were 99 17–36 yr old students who were in ongoing relationships and who had different attachment styles. Ss were compared in terms of the self-disclosures they directed toward their partner vs a stranger, using questionnaire and behavioural measures. Behavioural self-disclosure was assessed by having Ss give talks on different topics, while anticipating that either their current partner or an opposite-sex stranger would later hear it. Results show that Ss with a secure attachment style disclosed more intimately to their partners than to strangers; whereas those with insecure attachment styles did not. Moreover, secures disclosed more personal facts to their partners than to strangers and were also perceived as more comfortable while self-disclosing to partners than to strangers. Finally, support for the hypothesis that self-disclosure mediates the relation between attachment style and relationship satisfaction was found on one component of self-disclosure, facilitative disclosure, a component which included both reported self-disclosure to one's partner and self-rated ability to elicit disclosure from others. (PsycINFO Database Record (c) 2010 APA, all rights reserved)     

"Attachment style and relationship satisfaction: Test of a self-disclosure explanation": Erratum.

Reports an error in "Attachment style and relationship satisfaction: Test of a self-disclosure explanation" by J. Patrick R. Keelan, Karen K. Dion and Kenneth L. Dion (Canadian Journal of Behavioural Science/Revue canadienne des sciences du comportement, 1998[Jan], Vol 30[1], 24-35). Some figures were inadvertently omitted in two tables. The correct tables are provided in the erratum. (The following abstract of the original article appeared in record 1998-01141-003.) Explored a self-disclosure explanation for why those with a secure attachment style report greater relationship satisfaction than those with insecure attachment styles. Ss were 99 17–36 yr old students who were in ongoing relationships and who had different attachment styles. Ss were compared in terms of the self-disclosures they directed toward their partner vs a stranger, using questionnaire and behavioural measures. Behavioural self-disclosure was assessed by having Ss give talks on different topics, while anticipating that either their current partner or an opposite-sex stranger would later hear it. Results show that Ss with a secure attachment style disclosed more intimately to their partners than to strangers; whereas those with insecure attachment styles did not. Moreover, secures disclosed more personal facts to their partners than to strangers and were also perceived as more comfortable while self-disclosing to partners than to strangers. Finally, support for the hypothesis that self-disclosure mediates the relation between attachment style and relationship satisfaction was found on one component of self-disclosure, facilitative disclosure, a component which included both reported self-disclosure to one's partner and self-rated ability to elicit disclosure from others. (PsycINFO Database Record (c) 2010 APA, all rights reserved)     

The Simultaneous Developing and Fixing of Photographic Emulsions*

An improved developing-fixing bath incorporating Phenidone is described. Variables which must be controlled for best results from any one emulsion are discussed.     

Aspergillus terreus infective endocarditis on a porcine heterograft valve

We present the first reported case of Aspergillus endocarditis occurring on a porcine heterograft prosthesis and discuss the literature regarding both Aspergillus endocarditis and endocarditis on a porcine valve. A 65-year-old man underwent aortic valve replacement with a porcine heterograft. Several weeks later he exhibited signs of infection and then of valve failure. Upon removal, the porcine valve substance was found to be grossly infiltrated and destroyed by the Aspergillus organism. The porcine valve has an excellent reputation of being resistant to infection and relatively easy to sterilize by medical management alone. Several surveys attest to the low incidence of endocarditis. Because of our experience with the destruction caused by Aspergillus, however, and because antimicrobials generally fail to eradicate this organism, we urge prompt operation to remove the heterograft valve involved with infection by this organism. The valve diseased with Aspergillus should be replaced with a mechanical prosthesis. In the aortic position, extra-anatomic replacement should be considered to facilitate eradication of periannular infection postoperatively.     

Influence of dietary fat composition on intestinal absorption in the rat

Omega-3 fatty acids influence the function of the intestinal brush border membrane. For example, the omega-3 fatty acid eicosapentaenoic acid (20∶5ω3) has an antiabsorptive effect on jejunal uptake of glucose. This study was undertaken to determine whether the effect of feeding α-linolenic acid (18∶3ω3) or EPA plus docosahexaenoic acid (22∶6ω3) on intestinal absorption of nutrients was influenced by the major source of dietary lipid, hydrogenated beef tallow or safflower oil. Thein vitro intestinal uptake of glucose, fatty acids and cholesterol was examined in rats fed isocaloric diets for 2 weeks: beef tallow, beef tallow + linolenic acid, beef tallow + eicosapentaenoic acid/docosahexaenoic acid, safflower oil, safflower oil + linolenic acid, or safflower oil + eicosapentaenic acid/docosahexaenoic acid. Eicosapentaenoic acid/docosahexaenoic acid reduced jejunal uptake of 10 and 20 mM glucose only when fed with beef tallow, and not when fed with safflower oil. Linolenic acid had no effect on glucose uptake, regardless of whether it was fed with beef tallow or safflower oil. The jejunal uptake a long-chain fatty acids (18∶0, 18∶2ω6, 18∶3ω3, 20∶4ω6, 20∶5ω3 and 22∶6ω3) and cholesterol was lower in salfflower oil than with beef tallow. When eicosapentaenoic acid/docosahexaenoic acid was given with beef tallow (but not with safflower oil), there was lower uptake of 18∶0, 20∶5ω3 and cholesterol. The demonstration of the inhibitory effect of linolenic acid or eicosapentaenoic acid/docosahexaenoic acid on cholesterol uptake required the feeding of a saturated fatty acid diet (beef tallow). These changes in uptake were not explained by differences in the animals’ food intake, body weight gain or intestinal weight. Feeding safflower oil was associated with an approximately 25% increase in the jejunal and ileal mucosal surface area, but this increase was prevented by combining linolenic acid or eicosapentaenoic acid/docosahexaenoic acid with safflower oil. Different inhibitory patterns were observed when mixtures of fatty acids were present together in the incubation medium, rather than in the diet: for example, when 18∶0 was in the incubation medium with 20∶4ω6, the uptake of 20∶4ω6 was reduced, whereas the uptake was unaffected by 18∶2ω6 or 20∶5ω3. Thus, (1) the inhibitory effect of eicosapentaenoic acid/docosahexaenoic acid on jejunal uptake of glucose, fatty acids and cholesterol was influenced by the major dietary lipid, saturated (beef tallow) or polyunsaturated fatty acid (safflower oil); and (2) different omega-3 fatty acids (linolenic acid versus eicosapentaenoic acid/docosahexaenoic acid) have a variable influence on the intestinal absorption of nutrients.