Diurnal and spatial variation of xylem dielectric constant in Norway Spruce (Picea abies [L.] Karst.) as related to microclimate, xylem sap flow, and xylem chemistry

Spatial and temporal variations in vegetation dielectric properties strongly influence the microwave backscatter characteristics of forested landscapes. This paper examines the relationship between xylem tissue dielectric constant, xylem sap flux density, and xylem sap chemical composition as measured in the stems of two Norway Spruce (Picea abies [L.] Karst.) trees in the Fichtelgebirge region of Northern Bavaria, Germany. Dielectric constant and xylem sap flux were monitored continuously from June through October 1995, at several heights along the tree trunks. At the end of the measurement series, each tree was harvested, and its xylem sap extracted and analyzed to determine the concentrations of amino acids and cations. Results show that the sap flux density was correlated with vapor pressure deficit (VPD) at all heights in the stem. In contrast, the xylem tissue dielectric constant is influenced by VPD but can exhibit a significant temporal lag relative to changes in VPD. This lag varies with position along the tree trunk. The temporal variability of the dielectric constant is compared with both trees at several positions along the tree trunks. Results of xylem sap chemical analysis are presented. We show that spatial and temporal variability in the xylem tissue dielectric constant is influenced not only by water content, but by variations in xylem sap chemistry as well. This has important implications for microwave remote sensing of forested landscapes, as useful information may be acquired regarding stand physiology and water relations and where variations in dielectric properties within individual trees and across geographic areas can be significant error sources for forest inventory mapping.     

Progress in the development of polymer cholesteric liquid crystal flakes for display applications

Polymer cholesteric liquid crystal (PCLC) flake technology is being developed as an alternative display technology for flexible, reflective particle displays. The motion of PCLC flakes suspended in a host fluid can be controlled with an electric field, creating a way to electrically control the flakes' ability to brightly reflect light that is circularly polarized. The PCLC flake/host fluid dispersion has been successfully microencapsulated both in a polymer matrix and in gelatin micro-capsules. Microencapsulation will not only expand the applications scope of the technology, but also may aid in addressing some potential problem areas that are inherent to many forms of particle display technology. A second important development in PCLC flake technology involves the manufacture of shaped flakes based on soft lithography techniques. The size and shape of a flake impact its reorientation, and uniformly shaped flakes respond in a similar manner. The unique reflective properties of PCLC flakes also provide possible applications in areas such as optics and photonics, switchable ‘smart windows’ or conformal coatings, and information displays such as ‘electronic paper.’     

The OKT3 Antibody Response Study: a multicentre study of human anti-mouse antibody (HAMA) production following OKT3 use in solid organ transplantation

Human anti-murine antibody titres following patient exposure to the monoclonal antibody Orthoclone OKT3 (muromonab-CD3) are determined by laboratories using diverse analytical methods which are not standardized and whose concordance is not established. A multicentre study group therefore compared testing for IgG anti-OKT3 antibody among seven laboratories. A set of 270 sera was obtained from 30 heart, 30 kidney and 30 liver transplant recipients with no previous exposure to OKT3 who were receiving OKT3 for induction immunosuppression. Sera were collected from each patient prior to and at 24 +/- 2 days and 31 +/- 2 days following initial OKT3 exposure. Identical aliquots of all 270 sera were tested for IgG anti-OKT3 antibody by each laboratory. In addition, the limit of detection of each laboratory's method was estimated by titration of an affinity-purified IgG anti-OKT3 reference material of known concentration. Anti-OKT3 antibody formation differed greatly among the three organ groups. Cardiac patients demonstrated the least sensitization and almost exclusively lower titres, while kidney recipients had more frequent and higher titre antibody formation. Liver recipients yielded the highest sensitization rate and the most frequent high titre sera. Importantly, the seven laboratories differed widely in the number of pretreatment sera reported as positive (ranging from 0% to 41% among laboratories), the number of post-OKT3 sera reported as positive (17-63%), the number of post-OKT3 samples with titre > or = 1000 (2-31%), and the number of patients sensitized 19-69%). Concordance among laboratories was highly variable, with interlaboratory agreement ranging from 38% to 83% on the sample titres assigned to 180 post-OKT3 sera. Many of the discordant results were consistent with differences in the limit of detection of the analytical methods, which ranged from 0.19 microgram/ml to > or = 15 micrograms/ml, a nearly 100-fold difference among laboratories. This study demonstrated the presence of both good concordance and significant discordance among laboratories in determining human anti-mouse antibody titres, and demonstrated that common titre categories (100, 1000, 10,000) were not equivalent among laboratories. The level of concordance among methods should be considered when comparing anti-OKT3 antibody results from different centres and their correlation with clinical events. Universal comparative testing, patterned after proficiency testing programmes, is needed to assess differences among laboratories and to bring uniformity and a sound interpretative basis to this field of testing.     

Improved processing for oil well cement evaluation-a with theoretical and laboratory data

A processing technique to measure cement impedance from ultrasonic pulse-echo waveforms, called normalized single mode (NSM) processing, is described. The technique is based on a physical model that represents the transducer in terms of its continuous plane wave kernel. Narrowband filtering to process a single resonant mode and all processing parameters are normalized to the modal frequency. The performance of NSM and a digital simulation of the present analog commercial processing, W2/W1, is compared for several environmental effects: casing thickness, eccentering, and added Gaussian noise. The computation is based on waveforms from a mathematical model and laboratory data. Results on model data show that NSM processing significantly reduces the impedance errors due to thickness variations, eccentering, and noise. Results on laboratory data indicate that the techniques yield comparable results for smooth machined casings, but that NSM processing significantly reduces measurement error and variance in a sample of the actual oil field casing.     

Satellite radar remote sensing of seasonal growing seasons for boreal and subalpine evergreen forests

We evaluated whether satellite radar remote sensing of landscape seasonal freeze-thaw cycles provides an effective measure of active growing season timing and duration for boreal and subalpine evergreen forests. Landscape daily radar backscatter measurements from the SeaWinds scatterometer on-board the QuikSCAT satellite were evaluated across a regional network of North American coniferous forest sites for 2000 and 2001. Radar remote sensing measurements of the initiation and length of the growing season corresponded closely with both site measurements and ecosystem process model (BIOME-BGC) simulations of these parameters because of the sensitivity of the K_u-band scatterometer to snow cover freeze-thaw dynamics and associated linkages between growing season initiation and the timing of seasonal snowmelt. In contrast, remote sensing estimates of the timing of growing season termination were either weakly or not significantly associated with site measurements and model simulation results, due to the relative importance of light availability and other environmental controls on stand phenology in the fall. Regional patterns of estimated annual net primary production (NPP) and component photosynthetic and autotrophic respiration rates for the evergreen forest sites also corresponded favorably with remote sensing estimates of the seasonal timing of spring thaw and associated growing season length, indicating the importance of these parameters in determining spatial and temporal patterns of NPP and the potential utility of satellite radar remote sensing for regional monitoring of the terrestrial biosphere.     

Radar remote sensing of the spring thaw transition across a boreal landscape

The seasonal transition of the boreal forest between frozen and non-frozen conditions affects a number of ecosystem processes that cycle between winter dormant and summer active states. The relatively short K_u-band wavelength (2.14 cm) of the space-borne NASA scatterometer (NSCAT) is sensitive to changes in dielectric properties, associated with large-scale changes in the relative abundance and phase (frozen or thawed) of canopy and surface water. We used a temporal change detection analysis of NSCAT daily radar backscatter measurements to characterize the 1997 seasonal spring thaw transition period across the 10⁶ km² BOREAS study region of central Canada. In the spring, air temperature transitions from frozen to non-frozen conditions and surface observations of seasonal snow cover depletion were generally coincident with decreases in radar backscatter of more than 2.9 dB, regardless of regional landcover characteristics. We used a temporal classification of NSCAT daily differences from 5-day smoothed backscatter values to derive three simple indices describing the initiation, primary event and completion of the spring thaw transition period. Several factors had a negative impact on the relative accuracy of NSCAT-based results, including periodic gaps in NSCAT daily time-series information and a large (i.e., >2 cm day⁻¹) spring rainfall event. However, these results were generally successful in capturing the seasonal transition of the region from frozen to non-frozen conditions, based on comparisons with regional weather station network information. These results illustrate the potential for improved assessment of springtime phenology and associated ecosystem dynamics across high latitude regions, where field based and optical remote-sensing methods are substantially degraded by frequent cloud cover, low solar illumination and sparse surface weather station networks.     

RESTORING SALMON HABITAT FOR A CHANGING CLIMATE

An important question for salmon restoration efforts in the western USA is ‘How should habitat restoration plans be altered to accommodate climate change effects on stream flow and temperature?’ We developed a decision support process for adapting salmon recovery plans that incorporates (1) local habitat factors limiting salmon recovery, (2) scenarios of climate change effects on stream flow and temperature, (3) the ability of restoration actions to ameliorate climate change effects, and (4) the ability of restoration actions to increase habitat diversity and salmon population resilience. To facilitate the use of this decision support framework, we mapped scenarios of future stream flow and temperature in the Pacific Northwest region and reviewed literature on habitat restoration actions to determine whether they ameliorate a climate change effect or increase life history diversity and salmon resilience. Under the climate change scenarios considered here, summer low flows decrease by 35–75% west of the Cascade Mountains, maximum monthly flows increase by 10–60% across most of the region, and stream temperatures increase between 2 and 6°C by 2070–2099. On the basis of our literature review, we found that restoring floodplain connectivity, restoring stream flow regimes, and re‐aggrading incised channels are most likely to ameliorate stream flow and temperature changes and increase habitat diversity and population resilience. By contrast, most restoration actions focused on in‐stream rehabilitation are unlikely to ameliorate climate change effects. Finally, we illustrate how the decision support process can be used to evaluate whether climate change should alter the types or priority of restoration actions in a salmon habitat restoration plan. Copyright © 2012 John Wiley & Sons, Ltd.     

ESTIMATION OF JUVENILE SALMON HABITAT IN PACIFIC RIM RIVERS USING MULTISCALAR REMOTE SENSING AND GEOSPATIAL ANALYSIS

We conducted a regional classification and analysis of riverine floodplain physical features that represent key attributes of salmon rearing habitats. Riverine habitat classifications, including floodplain area and river channel complexity, were derived at moderate (30 m) spatial resolution using multispectral Landsat imagery and global terrain data (90 m) encompassing over 3 400 000 km² and most North Pacific Rim (NPR) salmon rivers. Similar classifications were derived using finer scale (i.e. ≤ 2.4‐m resolution) remote sensing data over a smaller set of 31 regionally representative flood plains. A suite of physical habitat metrics (e.g. channel sinuosity, nodes, floodplain width) were derived from each dataset and used to assess the congruence between similar habitat features at the different spatial scales and to evaluate the utility of moderate scale geospatial data for determining abundance of selected juvenile salmon habitats relative to fine scale remote sensing measurements. The resulting habitat metrics corresponded favorably (p < 0.0001) between the moderate scale and the fine scale floodplain classifications; a subset of these metrics (channel nodes and maximum floodplain width) also were strong indicators (R² > 0.5, p < 0.0001) of floodplain habitats defined from the finer scale analysis. These relationships were used to estimate the abundance and distribution of three critical shallow water floodplain habitats for juvenile salmon (parafluvial and orthofluvial springs, and shallow shore) across the entire NPR domain. The resulting database provides a potential tool to evaluate and prioritize salmon conservation efforts both within individual river systems and across major catchments on the basis of physical habitat distribution and abundance. Copyright © 2011 John Wiley & Sons, Ltd.     

Spatially Detailed Quantification of Metal Loading for Decision Making: Metal Mass Loading to American Fork and Mary Ellen Gulch,Utah

Effective remediation requires an understanding of the relative contributions of metals from all sources in a catchment, and that understanding must be based on a spatially detailed quantification of metal loading. A traditional approach to quantifying metal loading has been to measure discharge and chemistry at a catchment outlet. This approach can quantify annual loading and the temporal changes in load, but does not provide the needed spatial detail to evaluate specific sources, which is needed to support remediation decisions. A catchment or mass-loading approach provides spatial detail by combining tracer-injection and synoptic-sampling methods to quantify loading. Examples of studies in American Fork, Utah, and its tributary Mary Ellen Gulch illustrate this different approach. The mass-loading study in American Fork treated Mary Ellen Gulch as a single inflow. From that point of view, Mary Ellen Gulch was one of the greatest sources of Fe, Mn, Zn, and colloidal Pb loads to American Fork. But when Mary Ellen Gulch was evaluated in a separate catchment study, the detailed locations of metal loading were identified, and the extent of metal attenuation upstream from the mouth of Mary Ellen Gulch was quantified. The net, instantaneous load measured at the mouth of Mary Ellen Gulch for remediation planning would greatly underestimate the contributions of principal sources within the catchment. Extending the detailed sampling downstream from Mary Ellen Gulch indicated the possibility of diffuse groundwater inflow from Mary Ellen Gulch to American Fork. Comparing loads for Mary Ellen Gulch in the two studies indicates that metal loads could be substantially underestimated for planning purposes without the detailed catchment approach for the low-flow conditions in these studies. A mass-loading approach provides both the needed quantification of metal loading and the spatial detail to guide remediation decisions that would be the most effective in the catchments.