High-sensitivity mid-infrared heterodyne spectrometer with a tunable diode laser as a local oscillator

A new mid-IR heterodyne spectrometer, which is intended to be applied for atmospheric and astrophysical studies, is presented. The spectrometer uses a frequency-stabilized tunable diode laser as a local oscillator. Owing to the low output power of available single-mode diode lasers, a newly developed confocal-ring resonator, the diplexer, is used to superimpose the source signal efficiently with that of the local oscillator. Additionally, the diplexer serves as an optical filter that establishes controlled optical feedback between the laser diode and the detector, which allows stable laser operation with linewidths of the order of 1 MHz. The heterodyne signal from the HgCdTe detector is analyzed by means of a 1.4-GHz acousto-optical spectrometer. With this setup we find system temperatures as low as 4400 K (double sideband), that is, approximately a factor of 6 of the quantum limit.     

Bücherschau

Ohne Zusammenfassung VDI     

Everyday activity patterns and sensory functioning in old age.

In the present study the authors investigated the relationship between visual and auditory acuity and everyday activity functioning. Participants were 516 older adults (70–103 years; equal numbers of men and women) who were members of the age-stratified Berlin Aging Study. Two categories of everyday activity functioning, perceived competence with basic activities of daily living (BaCo; basic competence) and amount of participation in discretionary social and leisure tasks (ExCo; expanded competence), were examined. The results revealed that sensory acuity, particularly vision, was a significant predictor of both BaCo and ExCo (rs ranging from .32 to .47). Indeed, hearing and vision could explain most of the age-related variance in everyday activities. At the same time, in the context of a broader model, evidence for the differential prediction of BaCo and ExCo was found, although there was also evidence for strong general age-related predictive variance that was common to both measures. Discussion focuses on the role of sensory acuity constructs as mediators of age-related variance in psychological and behavioral outcomes and the potential causal implications of this mediation. (PsycINFO Database Record (c) 2010 APA, all rights reserved)     

Structural studies of binding site tryptophan mutants in the high-affinity streptavidin-biotin complex

Previous thermodynamic and computational studies have pointed to the important energetic role of aromatic contacts in generating the exceptional binding free energy of streptavidin-biotin association. We report here the crystallographic characterization of single site tryptophan mutants in investigating structural consequences of alterations in these aromatic contacts. Four tryptophan residues, Trp79, Trp92, Trp108 and Trp120, play an important role in the hydrophobic binding contributions, which along with a hydrogen bonding network and a flexible binding loop give rise to tight ligand binding (Ka approximately 10(13) M-1). The crystal structures of ligand-free and biotin-bound mutants, W79F, W108F, W120F and W120A, in the resolution range from 1.9 to 2.3 A were determined. Nine data sets for these four different mutants were collected, and structural models were refined to R-values ranging from 0.15 to 0.20. The major question addressed here is how these mutations influence the streptavidin binding site and in particular how they affect the binding mode of biotin in the complex. The overall folding of streptavidin was not significantly altered in any of the tryptophan mutants. With one exception, only minor deviations in the unbound structures were observed. In one crystal form of unbound W79F, there is a coupled shift in the side-chains of Phe29 and Tyr43 toward the mutation site, although in a different crystal form these shifts are not observed. In the bound structures, the orientation of biotin in the binding pocket was not significantly altered in the mutant complex. Compared with the wild-type streptavidin-biotin complex, there were no additional crystallographic water molecules observed for any of the mutants in the binding pocket. These structural studies thus suggest that the thermodynamic alterations can be attributed to the local alterations in binding residue composition, rather than a rearrangement of binding site architectures.     

Adverse Life Events in Late Life: Their Manifestation and Management in Daily Life.

The purpose of this study was to investigate the relationships among major life events, chronic events, daily instrumental activities, and well-being. Fifty individuals between 73 and 93 years of age reported major life events, minor hassles and uplifts, and daily activities during 5 measurement periods. In addition, positive affect was assessed in situ 5 times per day on 6 consecutive days on the basis of a random time-sampling scheme. The effects of major life events on positive affect were transmitted through minor events. However, there was no evidence that the relationship between minor events and positive affect was moderated by the occurrence of a major event. The relationship between life events and positive affect, however, was moderated by change in instrumental activities. (PsycINFO Database Record (c) 2010 APA, all rights reserved)     

THE REMOVAL OF THIN LIQUID FILMS FROM PERIODICALLY GROOVED SURFACES BY AN IMPINGING JET

A thin uniform film of viscous liquid, that initially covers a plane rigid surface, is removed under the shearing action of an impinging jet of water. Data are presented for the mean thickness of residual film as a function of time, for both smooth and grooved surfaces. An empirical correlation of the data, which exhibit a reduction in cleaning rate due to surface roughness, based on a simple theoretical model for the case of smooth surfaces, is presented. The correlation is discussed in the light of numerical simulations of shear-driven flow over surfaces which have periodic roughness.     

Energetic roles of hydrogen bonds at the ureido oxygen binding pocket in the streptavidin-biotin complex

The high-affinity streptavidin-biotin complex is characterized by an extensive hydrogen-bonding network. A study of hydrogen-bonding energetics at the ureido oxygen of biotin has been conducted with site-directed mutations at Asn 23, Ser 27, and Tyr 43. A new competitive biotin binding assay was developed to provide direct equilibrium measurements of the alterations in Kd. S27A, Y43F, Y43A, N23A, and N23E mutants display DeltaDeltaG degrees at 37 degrees C relative to wild-type streptavidin of 2.9, 1.2, 2.6, 3.5, and 2.6 kcal/mol, respectively. The equilibrium-binding enthalpies for all of the mutants were measured by isothermal titration calorimetry, and the Y43A and N23A mutants display large decreases in the equilibrium binding enthalpy at 25 degrees C of 8.9 and 6.9 kcal/mol, respectively. The S27A and N23E mutants displayed small decreases in binding enthalpy of 1.6 and 0.9 kcal/mol relative to wild-type, while the Y43F mutant displayed a -2.6 kcal/mol increase in the binding enthalpy at 25 degrees C. At 37 degrees C, the Y43A and N23A mutants display decreases of 7.8 and 7.9 kcal/mol, respectively, while the S27A, N23E, and Y43F mutants displayed decreases of 4.9, 3.7, and 1.2 kcal/mol relative to wild-type. Kinetic analyses were also conducted to probe the contributions of the hydrogen bonds to the activation barrier. Wild-type streptavidin at 37 degrees C displays a koff of (4.1 +/- 0.3) x 10(-5) s-1, and the conservative Y43F, S27A, and N23A mutants displayed increases in koff to (20 +/- 1) x 10(-5) s-1, (660 +/- 40) x 10(-5) s-1, and (1030 +/- 220) x 10(-)5 s-1, respectively. The Y43A and N23E mutants displayed 93-fold and 188-fold increases in koff, respectively. Activation energies and enthalpies for each of the mutants were determined by transition-state analysis of the dissociation rate temperature dependence. All of the mutants except Y43F display large reductions in the activation enthalpy. The Y43F mutant has a more positive activation enthalpy, and thus a more favorable activation entropy that underlies the overall reduction in the activation barrier. For the most conservative mutant at each ureido oxygen hydrogen-bonding position, bound-state alterations account for most of the energetic changes in a single transition-state model, suggesting that the ureido oxygen hydrogen-bonding interactions are broken in the dissociation transition state.