An in vitro model of traumatic neuronal injury: loading rate-dependent changes in acute cytosolic calcium and lactate dehydrogenase release

We developed a new in vitro model of neuronal injury using NT2-N cells to examine the effects of hydrodynamic loading rate on intraneuronal calcium dynamics and lactate dehydrogenase (LDH) release. Our apparatus consisted of a parallel disk viscometer which induced fluid shear stress with well-defined magnitudes and loading rates to cultured cells. We found that the deformation response of the cells was dependent on the severity of the insult, with increased cellular strains generated for higher shear stresses at a constant loading rate. Peak intracellular free calcium concentration correlated with strain, suggesting that mechanical deformation may regulate calcium response. Slowly applied fluid shear stress elicited no response, whereas high loading rates resulted in peak calcium increases 2.9 to 3.6 times baseline values as injury severity was increased. LDH release measured within 5 min after the insult correlated with loading rate. In addition, LDH release continued to increase out to 24 h following high loading rate conditions, demonstrating that the application of fluid shear stress led to prolonged cell damage. The acute response in NT2-N cells subjected to an insult with the CSID is dependent on the loading rate, and these results suggest that initial membrane deformation may trigger subsequent events.     

Increased messenger RNA from protein synthesis inhibited human fibroblasts

Numerous reports have demonstrated that specific protein synthesis in response to specific inducers is markedly stimulated by a simultaneous brief exposure to protein synthesis inhibitors such as cycloheximide. This phenomenon is known as "superinduction" and is most often attributed to the accumulation of cytoplasmic messenger RNA during the inhibition period. Messenger RNA, as defined by rapid labeling, oligo (dt)-cellulose binding, and cell free protein synthesis stimulation was measured in cycloheximide treated human fibroblasts. In spite of a consistent 40% decrease in total polysomal 3H-uridine labeled RNA, a 1.5- to 2-fold increase in extractable mRNA was observed. These data provide direct evidence that protein synthesis inhibition stimulates the appearance of cytoplasmic mRNA and/or completely blocks its degradation and, are consistent with the hypothesis that mRNA accumulation partly underlies the superinduction phenomena.     

500kV温南变电所接地网安全性问题的探讨

文章从接地阻抗、地电位升、接触电压和跨步电压的角度综合分析探讨了地处高土壤电阻率地区的500kV温南变电所接地网安全性问题,并提出了相应的安全防范措施,如通往变电所外的金属管加绝缘段隔离、通信电缆全部采用光缆、验算接触电压和跨步电压、降低电缆的感应电压以及避雷针采用集中接地等,最终使地网接地阻抗可放宽到0·5Ω仍能确保变电所安全运行和人身安全。     

图像分割方法的研究现状与展望

图像分割是图像处理中的一项关键技术，也是一经典难题，发展至今仍没有找到一个通用的方法，也没有制定出判断分割算法好坏的标准．对近几年来出现的图像分割方法作了较为全面的综述，探讨了图像分割技术的发展方向，对从事图像处理研究的科研人员具有一定的启发作用．     

蒸汽发生器传热管面内流体弹性失稳分析

流体弹性失稳是蒸汽发生器内最严重的传热管流致振动机理,一旦发生就会使传热管发生大幅振动并快速失效。流体弹性失稳可能在U形传热管束的面内及面外两个方向发生,为研究面内及面外流体弹性失稳发生的先后顺序,通过将蒸汽发生器U形传热管防振条支撑假设为单向简支,即仅在传热管面外方向对U形管进行约束,建立了完整的U形管模型;计算了弯曲半径及防振条支撑数量对U形管面内外固有频率的影响,基于成熟的流体弹性失稳经验模型,得到了面内流体弹性失稳先于面外方向发生的条件。结果表明,对弯曲半径在0.5 m-1.75 m范围内的U形传热管,当其弯管段支撑点超过4 h,面内流体弹性失稳将先于面外方向发生。     

支持关键词搜索的空间连接查询研究

近年来,许多实际应用不仅需要支持空间连接查询而且需要具备关键词搜索功能,以帮助用户查找那些既满足空间连接条件又包含指定关键词的空间对象组合.正是在这种需求的驱动之下,定义了一种具备关键词搜索功能的空间连接查询(Spatial Join with Keyword Search,缩写SJKS),并提出了一种基于IR2-Tree的SJKS查询处理算法(IR2-TreeSJKS算法),旨在实现关键词搜索与空间连接查询的高效结合.实验表明,本算法可有效支持具有关键词搜索功能的空间连接查询处理.     

一种基于Verilog HDL的OBS汇聚模块的实现方案

对光突发交换(OBS)网络的边缘节点进行了研究,给出了一种新型的OBS边缘节点汇聚模块的实现方案.与以往基于软件仿真的方法不同,该方案以FPGA芯片EP2C20Q240C8为平台,采用Verilog HDL语言编写实现.时序分析结果显示:该汇聚模块能够实现两种基本的汇聚算法(FAP和FBL),同时占用芯片资源和时延情况分析结果也能满足OBS网络的传输需求.